CREPT and p15RS are two recently identified homologous proteins that regulate cell proliferation in an opposite way and are closely related to human cancer development. Both CREPT and pl5RS consist of an N-terminal RP...CREPT and p15RS are two recently identified homologous proteins that regulate cell proliferation in an opposite way and are closely related to human cancer development. Both CREPT and pl5RS consist of an N-terminal RPR domain and a C-terminal domain with high sequence homology. The transcription enhancement by CREPT is attributed to its interaction with RNA polymerase II （Pol II）. Here we provide biochemical and structural evidence to support and extend this molecular mechanism. Through fluorescence polarization analysis, we show that the RPR domains of CREPT and pl5RS （CREPT-RPR and pI5RS-RPR） bind to different Pol II C-terminal domain （CTD） phosphoisoforms with similar affinity and specificity. We also determined the crystal structure of pl5RS-RPR. Sequence and structural comparisons with RPR domain of Rttl03, a homolog of CREPT and p l5RS in yeast, reveal structural basis for the similar binding profile of CREPT-RPR and p 15RS-RPR with Pol II CTD. We also determined the crystal structure of the C-terminal domain of CREPT （CREPT-CTD）, which is a long rod-like dimer and each monomer adopts a coiled-coil structure. We propose that dimerization through the C-terminal domain enhances the binding strength between CREPT or pl5RS with Pol II by increasing binding avidity. Our results collectively reveal the respective roles of N-terminal RPR domain and C-terminal domain of CREPT and pl5RS in recognizing RNA Pol II.展开更多
This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or a...This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or an end car, and Vehicle TP03, the third car counting from TC01, are measured for the train running at speeds from 260 km/h to 385 km/h, along two types of track including a slab track and a ballast track and either on the ground surface or in a tunnel. Data analyses are performed for sound pressure overall levels, frequency, area contributions, and possible generation mechanisms, showing how they are affected by train speed, running direction, track type, and tunnel. The results show that, whether TC01 is used as head car or end car, the interior noise characteristics in the VIP cabin are mostly related to aerodynamic noise. Differences in interior noise between tracks become smaller as the train speed increases. The effect of a tunnel on the interior noise is more important for the middle coach than that for the head coach. This study can provide a basis for noise control of high-speed trains.展开更多
基金supported by Ministry of Science and Technology(2010CB912402 and 2011CB910502)Ministry of Health(2012ZX1000-1009) and the Fok Ying Tung Education Foundation to Wang XinQuan+1 种基金the National Natural Science Foundation of China(81230044,81372167 and 31071225)the Tsinghua Science Foundation(20121080018)to Chang ZhiJie
文摘CREPT and p15RS are two recently identified homologous proteins that regulate cell proliferation in an opposite way and are closely related to human cancer development. Both CREPT and pl5RS consist of an N-terminal RPR domain and a C-terminal domain with high sequence homology. The transcription enhancement by CREPT is attributed to its interaction with RNA polymerase II （Pol II）. Here we provide biochemical and structural evidence to support and extend this molecular mechanism. Through fluorescence polarization analysis, we show that the RPR domains of CREPT and pl5RS （CREPT-RPR and pI5RS-RPR） bind to different Pol II C-terminal domain （CTD） phosphoisoforms with similar affinity and specificity. We also determined the crystal structure of pl5RS-RPR. Sequence and structural comparisons with RPR domain of Rttl03, a homolog of CREPT and p l5RS in yeast, reveal structural basis for the similar binding profile of CREPT-RPR and p 15RS-RPR with Pol II CTD. We also determined the crystal structure of the C-terminal domain of CREPT （CREPT-CTD）, which is a long rod-like dimer and each monomer adopts a coiled-coil structure. We propose that dimerization through the C-terminal domain enhances the binding strength between CREPT or pl5RS with Pol II by increasing binding avidity. Our results collectively reveal the respective roles of N-terminal RPR domain and C-terminal domain of CREPT and pl5RS in recognizing RNA Pol II.
基金Project supported by the National Natural Science Foundation of China （Nos. 51475390 and U 1434201）, the National Key Technology R＆D Program of China （Nos. 2016YFB1200506-08 and 2016YFB1200503-02）, and the Scientific Research Foundation of State Key Laboratory of Traction Power （No. 2015TPL_T08）, China
文摘This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or an end car, and Vehicle TP03, the third car counting from TC01, are measured for the train running at speeds from 260 km/h to 385 km/h, along two types of track including a slab track and a ballast track and either on the ground surface or in a tunnel. Data analyses are performed for sound pressure overall levels, frequency, area contributions, and possible generation mechanisms, showing how they are affected by train speed, running direction, track type, and tunnel. The results show that, whether TC01 is used as head car or end car, the interior noise characteristics in the VIP cabin are mostly related to aerodynamic noise. Differences in interior noise between tracks become smaller as the train speed increases. The effect of a tunnel on the interior noise is more important for the middle coach than that for the head coach. This study can provide a basis for noise control of high-speed trains.
