To improve the similarity measurement between users, a similarity measurement approach incorporating clusters of intrinsic user groups( SMCUG) is proposed considering the social information of users. The approach co...To improve the similarity measurement between users, a similarity measurement approach incorporating clusters of intrinsic user groups( SMCUG) is proposed considering the social information of users. The approach constructs the taxonomy trees for each categorical attribute of users. Based on the taxonomy trees, the distance between numerical and categorical attributes is computed in a unified framework via a proper weight. Then, using the proposed distance method, the nave k-means cluster method is modified to compute the intrinsic user groups. Finally, the user group information is incorporated to improve the performance of traditional similarity measurement. A series of experiments are performed on a real world dataset, M ovie Lens. Results demonstrate that the proposed approach considerably outperforms the traditional approaches in the prediction accuracy in collaborative filtering.展开更多
Tissue expression and stock variation of isozymes of stone flounder (Kareius bicoloratus) were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The result...Tissue expression and stock variation of isozymes of stone flounder (Kareius bicoloratus) were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The results indicated that all the isozymes examined were obviously tissue-specific. The expressions of SOD*, GDH*, G3PDH-2* and ADH-2* were detected only in liver, SDH-1*, MDH-1* and ADH-1* only in muscle, and LDH-B* and LDH-C* only in eyes. In comparison, MDH-2*, GPI-3* and SDH-2* were detected in all tissues examined. Other loci examined were detected in a variety of tissues. Muscle and liver were selected to detect the isozyme variation of the two geographic stocks of Qingdao and Weihai, Shandong Province, China. The percentages of polymorphic loci (P0.99) were 29.17% and 25.00%, the observed heterozygosities (H0) were 0.028 ±0.014 and 0.040 ± 0.019, and the expected heterozygosities (He) were 0.039±0.017 and 0.052±0.022 in Qingdao and Wethai stock, respectively. The coefficient of gene differentiation (Fst) and genetic distance (D) between the two stocks was 0.012 and 0.0011, respectively, indicating that the genetic differentiation is low between them. Compared with other species of Pleuronectiformes, both the percentage of polymorphic loci and the mean heterozygosity ofK. Bicoloratus were at a middle level.展开更多
Purpose of this work was to study changes in the ion balance under salt stress in roots and leaves of seedlings of T. aestivum L. and its wild congeners with different genomic composition. Laboratory assessment of sal...Purpose of this work was to study changes in the ion balance under salt stress in roots and leaves of seedlings of T. aestivum L. and its wild congeners with different genomic composition. Laboratory assessment of salt tolerance in seedlings was carried out and contents of ions K^+, Na^+ and Ca^2+ in roots and leaves were defined. Considerable change of ionic balance in roots and leaves of studied forms of wheat against salt stress is shown. It is noticed that the parity K^+/Na^+ decreases in process of strengthening of salt stress. Authentic negative correlation between a relative gain of a biomass of leaves and roots in the stress conditions and accumulation of ions Na^+ and between a relative gain of a biomass of leaves in the stress conditions and a parity in them of ions K^+/Na^+ is revealed. The research results also show that the stability characteristics of indicators of changes in ionic composition or balance in the leaves of young plants are more informative than in the roots and allow to reveal the form, characterized by relatively high of K^+/Na^+ ratio and the relatively low accumulation of Ca2^+ as the most resistant to salt stress.展开更多
Ecdysis is a comm on phe nomenon that happe ns throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii.It is vital to better understand the correlation between cannibalism and biochemical com...Ecdysis is a comm on phe nomenon that happe ns throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii.It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process.The objective of the present study was to determine the amino acid profile released by M.rosenbergii during the ecdysis process that promotes cannibalism.To accomplish this,changes in amino acid levels(total amino acid(TAA)and free amino acid(FAA))of tissue muscle,exoskeleton,and sample water of culture medium from the moulting(E-stage)and non-moulting(C-stage)prawns were analysed using highperformanee liquid chromatography(HPLC).Comparison study revealed that among the TAA compounds,proline and sarcosine of tissues from moulting prawn were found at the highest levels.The level of FAA from water that contains moulting prawns(E-stage)was dominated by tryptophan and proline.Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M.rosenbergii during ecdysis.The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism,and also for future dietary man ipulati on to improve feeding efficie ncies and feeding man agement,which in directly impacts productivity and profitability.展开更多
The oxygen isotope ratios (δ18O) preserved in marine sediments have been widely used to reconstruct past ocean temperatures. However, there remain significant uncertainties associated with this method, owing to ass...The oxygen isotope ratios (δ18O) preserved in marine sediments have been widely used to reconstruct past ocean temperatures. However, there remain significant uncertainties associated with this method, owing to assumptions about the δ18O of ancient seawater which affects the temperature inferred from sediment δ18O records. In this study, oxygen isotope compositions of phosphate in teeth and bones from five different modem cetacean species, including sperm whale, pygmy sperm whale, short-finned pilot whale, killer whale, and Cuvier's beaked whale, and three fossil whales were determined. The data were used to assess whether the oxygen isotope ratios of biogenic phosphate (δ18Op) from cetaceans are a reliable proxy for the oxygen isotopic composition of ocean water (δ18Ow). The δ18Op values of modem cetaceans range from 15.5 ‰ to 21.3 ‰, averaging (19.6 ‰ ±0.8 ‰) (n = 136).Using a greatly expanded global cetacean δ18Op dataset, the following regression equation is derived for cetaceans: δ18Ow = 0.95317 (4-0.03293) δ18Op- 17.971 (+0.605), r = 0.97253. The new equation, when applied to fossil teeth and bones, yielded reasonable estimates of ancient seawater δ18Ow values. Intra-tooth isotopic variations were observed within individual teeth. Among the selected species, the killer whale (O. orca) has largest intra-tooth δ18Op preference and migratory the lowest δ18Op values and the variation, reflecting its habitat behavior. The results show that oxygen isotope analysis of phosphate in cetacean teeth and dense ear bones provides a useful tool for reconstructing the oxygen isotopic composition of seawater and for examining environmental preferences (including migratory behavior) of both modem and ancient whales.展开更多
基金The National High Technology Research and Development Program of China(863 Program)(No.2013AA013503)the National Natural Science Foundation of China(No.61472080+3 种基金6137020661300200)the Consulting Project of Chinese Academy of Engineering(No.2015-XY-04)the Foundation of Collaborative Innovation Center of Novel Software Technology and Industrialization
文摘To improve the similarity measurement between users, a similarity measurement approach incorporating clusters of intrinsic user groups( SMCUG) is proposed considering the social information of users. The approach constructs the taxonomy trees for each categorical attribute of users. Based on the taxonomy trees, the distance between numerical and categorical attributes is computed in a unified framework via a proper weight. Then, using the proposed distance method, the nave k-means cluster method is modified to compute the intrinsic user groups. Finally, the user group information is incorporated to improve the performance of traditional similarity measurement. A series of experiments are performed on a real world dataset, M ovie Lens. Results demonstrate that the proposed approach considerably outperforms the traditional approaches in the prediction accuracy in collaborative filtering.
文摘Tissue expression and stock variation of isozymes of stone flounder (Kareius bicoloratus) were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The results indicated that all the isozymes examined were obviously tissue-specific. The expressions of SOD*, GDH*, G3PDH-2* and ADH-2* were detected only in liver, SDH-1*, MDH-1* and ADH-1* only in muscle, and LDH-B* and LDH-C* only in eyes. In comparison, MDH-2*, GPI-3* and SDH-2* were detected in all tissues examined. Other loci examined were detected in a variety of tissues. Muscle and liver were selected to detect the isozyme variation of the two geographic stocks of Qingdao and Weihai, Shandong Province, China. The percentages of polymorphic loci (P0.99) were 29.17% and 25.00%, the observed heterozygosities (H0) were 0.028 ±0.014 and 0.040 ± 0.019, and the expected heterozygosities (He) were 0.039±0.017 and 0.052±0.022 in Qingdao and Wethai stock, respectively. The coefficient of gene differentiation (Fst) and genetic distance (D) between the two stocks was 0.012 and 0.0011, respectively, indicating that the genetic differentiation is low between them. Compared with other species of Pleuronectiformes, both the percentage of polymorphic loci and the mean heterozygosity ofK. Bicoloratus were at a middle level.
文摘Purpose of this work was to study changes in the ion balance under salt stress in roots and leaves of seedlings of T. aestivum L. and its wild congeners with different genomic composition. Laboratory assessment of salt tolerance in seedlings was carried out and contents of ions K^+, Na^+ and Ca^2+ in roots and leaves were defined. Considerable change of ionic balance in roots and leaves of studied forms of wheat against salt stress is shown. It is noticed that the parity K^+/Na^+ decreases in process of strengthening of salt stress. Authentic negative correlation between a relative gain of a biomass of leaves and roots in the stress conditions and accumulation of ions Na^+ and between a relative gain of a biomass of leaves in the stress conditions and a parity in them of ions K^+/Na^+ is revealed. The research results also show that the stability characteristics of indicators of changes in ionic composition or balance in the leaves of young plants are more informative than in the roots and allow to reveal the form, characterized by relatively high of K^+/Na^+ ratio and the relatively low accumulation of Ca2^+ as the most resistant to salt stress.
基金Fundamental Research Grant Scheme from the Ministry of Higher Education,Malaysia(No.59324)。
文摘Ecdysis is a comm on phe nomenon that happe ns throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii.It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process.The objective of the present study was to determine the amino acid profile released by M.rosenbergii during the ecdysis process that promotes cannibalism.To accomplish this,changes in amino acid levels(total amino acid(TAA)and free amino acid(FAA))of tissue muscle,exoskeleton,and sample water of culture medium from the moulting(E-stage)and non-moulting(C-stage)prawns were analysed using highperformanee liquid chromatography(HPLC).Comparison study revealed that among the TAA compounds,proline and sarcosine of tissues from moulting prawn were found at the highest levels.The level of FAA from water that contains moulting prawns(E-stage)was dominated by tryptophan and proline.Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M.rosenbergii during ecdysis.The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism,and also for future dietary man ipulati on to improve feeding efficie ncies and feeding man agement,which in directly impacts productivity and profitability.
基金supported by US National Science Foundation Cooperative Agreement No.DMR-1157490 and the State of Floridathe State of Florida.The Stable Isotope Lab was established with grants from the US National Science Foundation(EAR-0824628EAR-0517806 and EAR-0236357)
文摘The oxygen isotope ratios (δ18O) preserved in marine sediments have been widely used to reconstruct past ocean temperatures. However, there remain significant uncertainties associated with this method, owing to assumptions about the δ18O of ancient seawater which affects the temperature inferred from sediment δ18O records. In this study, oxygen isotope compositions of phosphate in teeth and bones from five different modem cetacean species, including sperm whale, pygmy sperm whale, short-finned pilot whale, killer whale, and Cuvier's beaked whale, and three fossil whales were determined. The data were used to assess whether the oxygen isotope ratios of biogenic phosphate (δ18Op) from cetaceans are a reliable proxy for the oxygen isotopic composition of ocean water (δ18Ow). The δ18Op values of modem cetaceans range from 15.5 ‰ to 21.3 ‰, averaging (19.6 ‰ ±0.8 ‰) (n = 136).Using a greatly expanded global cetacean δ18Op dataset, the following regression equation is derived for cetaceans: δ18Ow = 0.95317 (4-0.03293) δ18Op- 17.971 (+0.605), r = 0.97253. The new equation, when applied to fossil teeth and bones, yielded reasonable estimates of ancient seawater δ18Ow values. Intra-tooth isotopic variations were observed within individual teeth. Among the selected species, the killer whale (O. orca) has largest intra-tooth δ18Op preference and migratory the lowest δ18Op values and the variation, reflecting its habitat behavior. The results show that oxygen isotope analysis of phosphate in cetacean teeth and dense ear bones provides a useful tool for reconstructing the oxygen isotopic composition of seawater and for examining environmental preferences (including migratory behavior) of both modem and ancient whales.