期刊文献+
共找到9篇文章
< 1 >
每页显示 20 50 100
由不同细胞核背景引起的小麦细胞质雄性不育系(CMS)叶绿体蛋白质组的变化(简报) 被引量:3
1
作者 张维佳 王艳 +6 位作者 李雪梅 范宝莉 王振英 彭永康 解超杰 刘志勇 孙其信 《分子细胞生物学报》 CSCD 北大核心 2007年第1期84-90,共7页
叶绿体和线粒体是高等植物细胞内2种重要的细胞器。由于细胞质雄性不育(CMS)被认为是一种由线粒体基因编码的性状,因此,近10多年来,国内外研究者对线粒体基因组结构与功能、由线粒体基因编码的与CMS相关蛋白的研究积累了大量的资... 叶绿体和线粒体是高等植物细胞内2种重要的细胞器。由于细胞质雄性不育(CMS)被认为是一种由线粒体基因编码的性状,因此,近10多年来,国内外研究者对线粒体基因组结构与功能、由线粒体基因编码的与CMS相关蛋白的研究积累了大量的资料。与线粒体相比。叶绿体与CMS关系的研究相对滞后。虽然一些研究者在核不育水稻中。观察到41kDa、45kDa、61kDa、P61、RPL1等叶绿体特异蛋白质,但对生产上有应用价值的细胞质、细胞核互作型雄性不育系中,仅对叶片全蛋白作过分析,而与CMS特性相关叶绿体特异蛋白的研究。迄今国内外未见报道。本研究以2套细胞质相同、细胞核不同的T型小麦雄性不育系、保持系为材料,利用2D—PAGE技术,对苗期、分蘖期、拔节期和孕穗期叶绿体蛋白质组进行比较分析。探讨不同细胞核背景下,CMS及相应保持系不同发育时期叶绿体蛋白质组变化;CMS特性与叶绿体蛋白质组的关系以及不同细胞核背景对叶绿体蛋白质组的影响等问题,本文报道了这方面的实验结果。 展开更多
关键词 同质异核 2D—PAGE 叶绿体蛋白质组 细胞质雄性不育 小麦
下载PDF
玉米雄性不育系的质、核对农艺性状的影响 被引量:4
2
作者 曹墨菊 荣廷昭 《四川农业大学学报》 CSCD 1997年第4期440-444,共5页
选用一组同核异质、四组同质异核不育系及保持系分别与测验系组成不完全双列杂交。对测交F1的吐丝期、株高、穗粒重等农艺性状进行了考察分析。结果表明:①同核异质组呈现出不同程度的胞质效应;同质异核组不同核背景之间表现出的差... 选用一组同核异质、四组同质异核不育系及保持系分别与测验系组成不完全双列杂交。对测交F1的吐丝期、株高、穗粒重等农艺性状进行了考察分析。结果表明:①同核异质组呈现出不同程度的胞质效应;同质异核组不同核背景之间表现出的差异较为明显。大多数性状的核基因组变异大于胞质基因组变异。②不育系在对农艺性状的影响上存在有质核互作效应。 展开更多
关键词 玉米 细胞质雄性不育 同质异核
下载PDF
多胞质、多核背景下玉米细胞质雄性不育系及其保持系的同工酶分析 被引量:3
3
作者 曹墨菊 荣廷昭 《四川农业大学学报》 CSCD 1998年第4期407-413,共7页
对玉米同核异质和同质异核两套不育系及其相应保持系单核至双核期的花药,采用薄层垂直平板聚丙烯酰胺凝胶不连续电泳及光密度扫描方法进行酯酶和过氧化物酶同工酶分析。结果表明:这两种酶的同工酶在同一核背景下的不胞质之间和同一胞... 对玉米同核异质和同质异核两套不育系及其相应保持系单核至双核期的花药,采用薄层垂直平板聚丙烯酰胺凝胶不连续电泳及光密度扫描方法进行酯酶和过氧化物酶同工酶分析。结果表明:这两种酶的同工酶在同一核背景下的不胞质之间和同一胞质下的不同核背景之间均表现出不同程度的差异,各类不育系与其相应保持系之间也表现出一定程度的差异。结合试验结果文中还对质核互作雄性不育产生的机理进行了讨论。 展开更多
关键词 玉米 同质异核 细胞质雄性不育 同工酶
下载PDF
玉米雄性不育细胞质特异DNA片段的发现及差异展示
4
作者 曹墨菊 荣廷昭 朱英国 《遗传》 CAS CSCD 北大核心 2005年第5期747-752,共6页
利用3对线粒体引物对玉米同核异质和同质异核不育系的基因组总DNA进行PCR扩增;对检测到多态性的引物,再分别对供试材料小孢子发育至四分体、单核期和双核期的花药总RNA进行差异显示分析。结果表明:以基因组总DNA为模板,引物P1-P2在所有... 利用3对线粒体引物对玉米同核异质和同质异核不育系的基因组总DNA进行PCR扩增;对检测到多态性的引物,再分别对供试材料小孢子发育至四分体、单核期和双核期的花药总RNA进行差异显示分析。结果表明:以基因组总DNA为模板,引物P1-P2在所有供试不育材料都有一相同的特异扩增带,而在保持系中均无扩增;引物P3-P4在所有供试材料中均无扩增;引物P5-P6仅在保持系黄早四中有扩增,而在其他供试材料中无扩增。这一结果说明以P1-P2为引物所检测到的特异扩增带为所有供试不育细胞质所特有,且不受供试材料不同核背景的影响。对于在不育材料基因组总DNA中具有特异扩增的引物P1-P2,进一步以cDNA为模板进行PCR扩增(RT-PCR),所有不育材料在小孢子发育的3个时期均有一相同的特异扩增带,而保持系在小孢子发育的相应时期均无扩增,说明以P1-P2为引物所检测到的转录本的大小和数目,在同核异质及同质异核不育材料间均表现一致,且不受小孢子发育时期的影响。这说明以P1-P2为引物所检测到的不育材料DNA水平的共同结构特点在小孢子发育中具有转录上的一致性,因此可以认为供试不育细胞质DNA水平的这一特异序列结构与雄性不育性状的表现有关。 展开更多
关键词 玉米 细胞质雄性不育 同质异核 PCR扩增 展示
下载PDF
玉米雄性不育系M3号和M4号的选育 被引量:1
5
作者 李正祥 孙云 +3 位作者 詹虹 龚建国 孙谦 李洪亮 《宁夏农林科技》 2015年第5期1-2,共2页
在玉米雄性不育系M1号、M2号选育的基础上,根据雄性不育系的遗传规律,介绍了M3号和M4号的选育过程,并对这2个新的不育系进行了简单的遗传组成分析和综合性状的测定。
关键词 不育系 细胞交换 遗传组成 同质异核不育系
下载PDF
Mixed Symmetry Isomeric States in Nuclei 被引量:3
6
作者 LONGGui-Lu ZHAOEn-Guang 《Communications in Theoretical Physics》 SCIE CAS CSCD 2002年第1期75-82,共8页
Mixed symmetry states are studied in the framework of the neutron-proton interacting boson model. It is found that some of the mixed symmetry states with moderate high spins change very fast with respect to the Majora... Mixed symmetry states are studied in the framework of the neutron-proton interacting boson model. It is found that some of the mixed symmetry states with moderate high spins change very fast with respect to the Majorana interaction. Under certain conditions, they become the yrast state or yrare state. These states are difficult to decay and become very stable. This study suggests that a possible new mode of isomers may exist due to the special nature in their proton and neutron degrees of freedom. 展开更多
关键词 mixed symmetry states isomeric states neutron-proton interacting boson model
下载PDF
Anomalously Large Deformation in Some Medium and Heavy Nuclei
7
作者 CHEN Ding-Han REN Zhong-Zhou TAI Fei 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第4X期687-696,共10页
We investigate the properties of the Ce isotopes with neutron number N =60 - 90 and the properties of the heavy nuclei near 242Am within the framework of deformed relativistic mean-field (RMF) theory. A systematic c... We investigate the properties of the Ce isotopes with neutron number N =60 - 90 and the properties of the heavy nuclei near 242Am within the framework of deformed relativistic mean-field (RMF) theory. A systematic comparison between theoretical results and experimental data is made. The calculated binding energies, two-neutron separation energies, and two-proton separation energies are in good agreement with experimental ones. The variation trend of experimental quadrupole deformation parameters on the Ce isotopes can be approximately reproduced by the RMF model. It is found that there exists an abnormally large deformation in the ground state of proton-rich Ce isotopes. This phenomenon can be the general behavior of proton-rich nuclei on the neighboring isotopic chains such as Nd and Sin isotopes. For the heavy nuclei near ^242 Am the properties of the ground state and superdeformed isomeric state can be approximately reproduced by the RMF model. The mechanism of the appearance of anomalously large deformation or superdeformation is analyzed and its influence on nuclear properties is discussed. Parther experiments to study the anomalously large deformation in some proton-rich nuclei are suggested. 展开更多
关键词 SUPERDEFORMATION proton-rich Ce nuclei isomeric state of ^242Am constraint relativistic meanfield calculation
下载PDF
Carbon nanotube@silicon carbide coaxial heterojunction nanotubes as metal-free photocatalysts for enhanced hydrogen evolution 被引量:2
8
作者 Xunfu Zhou Qiongzhi Gao +1 位作者 Siyuan Yang Yueping Fang 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2020年第1期62-71,共10页
Considerable research efforts have been devoted to developing novel photocatalysts with increased performances by hybridizing inorganic nanomaterials with carbon nanotubes.In this work,one-dimensional coaxial core-she... Considerable research efforts have been devoted to developing novel photocatalysts with increased performances by hybridizing inorganic nanomaterials with carbon nanotubes.In this work,one-dimensional coaxial core-shell carbon nanotubes@SiC nanotubes were successfully synthesized via in situ growth of SiC coatings on carbon nanotubes by a vapor-solid reaction between silicon vapor and carbon nanotubes.High-resolution transmission electron microscope images show that SiC and carbon nanotubes link to form a robust heterojunction with intrinsic atomic contact,which results in efficient separation of the photogenerated electron-hole pairs on SiC and electron transfer from SiC to carbon nanotubes.Compared with those of similar materials such as pure SiC nanocrystals and SiC nanotubes,the metal-free carbon nanotubes@SiC exhibits an enhanced photocatalytic activity for hydrogen evolution,which is attributed to the enhanced light absorption and the efficient interfacial charge transfer/separation brought about by their one-dimensional coaxial nanoheterostructures.Moreover,the photocatalytic stability of the metal-free carbon nanotubes@SiC was tested for over 20 h without any obvious decay. 展开更多
关键词 Silicon carbide Coaxial core-shell nanotubes Nanoheterostructures Charge separation Hydrogen evolution
下载PDF
Oblate Superdeformed High-K States in Superheavy Nuclei
9
作者 刘红亮 《Communications in Theoretical Physics》 SCIE CAS CSCD 2013年第11期577-580,共4页
Configuration-constrained calculations of potential-energy surfaces for ^292 122 show the occurrence of multiquasiparticle high-K isomeric state at oblate superdeformation. Such state could play a unique role in super... Configuration-constrained calculations of potential-energy surfaces for ^292 122 show the occurrence of multiquasiparticle high-K isomeric state at oblate superdeformation. Such state could play a unique role in superheavy nuclei, with possible long life time from enhanced difficulty in fission due to additional barrier at oblate deformation, retardation in a decay due to unpaired nucleons, and hindrance in γ-ray transition due to K forbiddenness. 展开更多
关键词 oblate deformation SUPERDEFORMATION K isomer superheavy nucleus
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部