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盐胁迫下小麦耐盐突变体后代差异cDNA的分离和鉴定 被引量:15
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作者 陈桂平 黄占景 +1 位作者 马闻师 沈银柱 《中国农业科学》 CAS CSCD 北大核心 2003年第9期996-999,共4页
采用cDNA AFLP(cDNA amplifiedfragmentlengthpolymorphism)技术分析了遗传背景相近的小麦后代突变体中 ,耐盐性较强的RH870 6 4 9(saltresistant,SR)和盐敏感的H870 6 34(saltsensitive ,SS)在盐胁迫和非胁迫情况下基因表达的差异。... 采用cDNA AFLP(cDNA amplifiedfragmentlengthpolymorphism)技术分析了遗传背景相近的小麦后代突变体中 ,耐盐性较强的RH870 6 4 9(saltresistant,SR)和盐敏感的H870 6 34(saltsensitive ,SS)在盐胁迫和非胁迫情况下基因表达的差异。结果表明 ,其中 88.1%的条带在 4个样品中是一致的 ,只有 11.9%的条带在 4个样品中表现出差异。选取其中的 6 8个差异条带进行克隆 ,测序 35个 ,经Internet进行Blast比较 ,发现 11个片段 (31.4 % )与已知基因具有较高的同源性 ,主要涉及与离子转运有关的蛋白、与信号转导有关的蛋白以及与氧化胁迫有关的蛋白 ;另外2 4个片段 (6 8.6 % )与已知基因同源性较低或未发现同源性 ,可能是一些未知基因。 展开更多
关键词 盐胁迫 小麦 耐盐突变体 后代差异 CDNA 分离鉴定 克隆 基因测序
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SPECIAL ISSUE: MIGRANT WORKERS IN THE COURSE OF URBANIZATION An investigation into intergenerational differences between two generations of migrant workers 被引量:14
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作者 Wang Xingzhou 《Social Sciences in China》 2008年第3期136-156,共21页
Among Chinese migrant workers, there are obvious intergenerational differences between those who were born before and after the dividing line of 1980. The latter possess more human capital and have plans for their car... Among Chinese migrant workers, there are obvious intergenerational differences between those who were born before and after the dividing line of 1980. The latter possess more human capital and have plans for their careers. In seeking work they tend to rely on government and the market, and they are more concerned with their long-term development. Their job stability is often quite low. They are more subject to infringement on their rights or more prone to believe that their rights have been infringed, but have a better knowledge of labor law and are bolder in the defense of their rights. Their individual consumption level is higher than that of the pre-1980 cohort, and they have a higher proportion of Intemet users. This generation is more likely to spend their spare time in recreation and entertainment. Their evaluation of and identification with society is relatively high, but they have a weakening connection with their hometowns and are therefore more willing to give up their contracted land and switch their household registration to the cities where they work. This generation of migrant workers poses a challenge not only to enterprises' ideas on management, but also to the dual urbanrural social structure. Having obtained the rights to migrate to and work in the cities, migrant workers should further obtain citizenship rights on a completely equal footing with urban residents. 展开更多
关键词 migrant workers POST-80S intergenerational difference CITIZENSHIP
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