Objective: To study the proliferation, migration and metaplasm of residual rabbit lens epithelial cells (LECs) after extracapsular cataract extraction(ECCE)based on the rabbit capsular bag model in vitro. Methods:...Objective: To study the proliferation, migration and metaplasm of residual rabbit lens epithelial cells (LECs) after extracapsular cataract extraction(ECCE)based on the rabbit capsular bag model in vitro. Methods: Sham cataract surgery, including anterior capsulorhexis, nucleus hydroexpression and aspiration of lens fibers, was performed on 20 rabbit lens. The capsular bags were isolated and pinned to sterile non-toxic silicone rings on petri dishes. The capsular bags were incubated with Eagle's minimum essential medium (DMEM) supplemented with 10% fetal calf serum (FCS) and monitored for 3 weeks by phase-contrast microscopy, after which light microscopy was performed on them.Results: After a latent period of 2-3 d, outgrowth was observed across the posterior capsule. Growth proceeded rapidly so that the posterior capsule was totally covered by a confluent monolayer of cell at 6-8 day. Capsular wrinkles became increasingly apparent as time progressed, causing a marked rise in light scatter. An increase in capsular tension also came.Conclusion: This model exhibits many of the in vito characteristics of the lens capsule after extracapsular surgery and may prove useful in further elucidating the cellular mechanisms of posterior capsule opacification and developing strategies for inhibiting cell growth with this system.展开更多
In this letter a Monte Carlo(MC) algorithm is used to simulate the propagation of polarized light in double-layer turbid media and the 2-dimentional backscattered Stokes vectors and Mueller matrices are obtained.Relat...In this letter a Monte Carlo(MC) algorithm is used to simulate the propagation of polarized light in double-layer turbid media and the 2-dimentional backscattered Stokes vectors and Mueller matrices are obtained.Relationships between backscattered Mueller matrix and optical properties,such as scattering,absorption and layered structure,are discussed in detail.Integrating the 2-dimentional Mueller matrix elements along radial and azimuthal directions,we obtain a reverse trend with respect to the optical parameters for upper and lower layers,which suggests possibilities for discriminating subtle optical properties in a double-layer structure using backscattered polarization patterns such as Mueller matrix.展开更多
文摘Objective: To study the proliferation, migration and metaplasm of residual rabbit lens epithelial cells (LECs) after extracapsular cataract extraction(ECCE)based on the rabbit capsular bag model in vitro. Methods: Sham cataract surgery, including anterior capsulorhexis, nucleus hydroexpression and aspiration of lens fibers, was performed on 20 rabbit lens. The capsular bags were isolated and pinned to sterile non-toxic silicone rings on petri dishes. The capsular bags were incubated with Eagle's minimum essential medium (DMEM) supplemented with 10% fetal calf serum (FCS) and monitored for 3 weeks by phase-contrast microscopy, after which light microscopy was performed on them.Results: After a latent period of 2-3 d, outgrowth was observed across the posterior capsule. Growth proceeded rapidly so that the posterior capsule was totally covered by a confluent monolayer of cell at 6-8 day. Capsular wrinkles became increasingly apparent as time progressed, causing a marked rise in light scatter. An increase in capsular tension also came.Conclusion: This model exhibits many of the in vito characteristics of the lens capsule after extracapsular surgery and may prove useful in further elucidating the cellular mechanisms of posterior capsule opacification and developing strategies for inhibiting cell growth with this system.
基金supported by the Governmental Education Bureau of Fujian Province(No.JA10068)the 2009 Project for Scientific and Technical Development of Xiamen (No.3502Z20099007)
文摘In this letter a Monte Carlo(MC) algorithm is used to simulate the propagation of polarized light in double-layer turbid media and the 2-dimentional backscattered Stokes vectors and Mueller matrices are obtained.Relationships between backscattered Mueller matrix and optical properties,such as scattering,absorption and layered structure,are discussed in detail.Integrating the 2-dimentional Mueller matrix elements along radial and azimuthal directions,we obtain a reverse trend with respect to the optical parameters for upper and lower layers,which suggests possibilities for discriminating subtle optical properties in a double-layer structure using backscattered polarization patterns such as Mueller matrix.
