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转染人TBX18基因的大鼠心脏成纤维细胞中HCN4蛋白表达、If及对心肌细胞搏动频率的影响观察
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作者 权大君 曹政 +1 位作者 杨媚 李莎 《山东医药》 CAS 北大核心 2016年第47期21-24,共4页
目的:观察转染人TBX18基因的大鼠心脏成纤维细胞HCN4蛋白表达、超极化激活内向离子电流(If)及对心肌细胞搏动频率的影响。方法取新生SD大鼠,断头处死后取心脏,分离培养成纤维细胞及心肌细胞。构建人TBX18基因的腺病毒载体Ad-GFP-TB... 目的:观察转染人TBX18基因的大鼠心脏成纤维细胞HCN4蛋白表达、超极化激活内向离子电流(If)及对心肌细胞搏动频率的影响。方法取新生SD大鼠,断头处死后取心脏,分离培养成纤维细胞及心肌细胞。构建人TBX18基因的腺病毒载体Ad-GFP-TBX18,包装并纯化病毒滴度至1&#215;1010 TU/mL。取传代2~5代时对数生长期新生大鼠成纤维细胞分为A、B、C组,培养24 h后A、B组分别转染Ad-GFP-TBX18、腺病毒载体pHBAd-MCMV-GFP(阴性对照),C组不做任何处理。采用RT-PCR法检测各组细胞TBX18 mRNA。采用Western blotting法检测各组超极化激活环核苷酸门控阳离子通道4(HCN4)蛋白。采用电压钳记录各组细胞超极化激活内向离子电流(If)。将各组细胞与新生大鼠心肌细胞共培养,观察新生大鼠心肌细胞搏动频率。结果 A、B、C组TBX18 mRNA相对表达量分别为98.32±6.21、1.03±0.05、1.02±0.02。A组TBX18 mRNA相对表达量高于B、C组(P均<0.05);B、C 组TBX18 mRNA相对表达量差异无统计学意义。A、B、C组HCN4蛋白相对表达量分别为0.49±1.21、0.12±0.02、0.09±0.01。A组HCN4蛋白相对表达量高于B、C组(P均<0.05);B、C组HCN4蛋白相对表达量差异无统计学意义。A组约有30%的细胞可以检测到If,而B、C组中没有细胞检测到If。同一时点A组新生大鼠心肌细胞搏动频率高于B、C组(P均<0.05);B、C组新生大鼠心肌细胞搏动频率差异无统计学意义。结论转染人TBX18基因的大鼠心脏成纤维细胞高表达HCN4蛋白,存在If电流,并能促进共培养新生大鼠心肌细胞的搏动。 展开更多
关键词 TBX18基因 基因转染 成纤维细胞 心肌细胞 超级化激活环核苷酸门控阳离子通道4 超极化激活内 向离子电流 心脏起博 动物实验
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A Mathematical Model for Pulsating Flow of Ionic Fluid under an Axial Electric Field: EMF Effect on Blood Flow
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作者 Mona A. EI-Naggar Medhat A. Messiery 《Journal of Physical Science and Application》 2012年第4期61-70,共10页
The present work introduces a mathematical model for ionic fluid that flows under the effect of both pulsating pressure and axial electromagnetic field. The fluid is treated as a Newtonian fluid applying Navier-Stokes... The present work introduces a mathematical model for ionic fluid that flows under the effect of both pulsating pressure and axial electromagnetic field. The fluid is treated as a Newtonian fluid applying Navier-Stokes equation. The fluid is considered as a neutral mixture of positive and negative ions. The effect of axial electric field is investigated to determine velocity profiles. Hydroelectric equation of the flow is deduced under dc and ac external electric field. Hence the effect of applied frequency (0-1 GHz) and amplitude (10-350 V/m) is illustrated. The ultimate goal is to approach the problem of EMF field interaction with blood flow. The applied pressure waveform is represented as such to simulate the systolic-diastolic behavior. Simulation was carried out using Maple software using blood plasma parameters; hence velocity profiles under various conditions are reported. 展开更多
关键词 Newtonian fluid navier-stokes ionic fluid blood flow electric field hydroelectric flow simulation velocity profiles EMF bioeffects.
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The relations between density of FACs in the Plasma Sheet Boundary Layers and Kp index 被引量:8
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作者 CHENG ZhengWei SHI JianKui +2 位作者 ZHANG TieLong DUNLOP Malcolm LIU ZhenXing 《Science China(Technological Sciences)》 SCIE EI CAS 2011年第11期2987-2992,共6页
We have studied 172 field-aligned currents (FACs) cases observed by the ClusterlI satellites when they crossed the plasma sheet boundary layer (PSBL) in the magnetotail from July to October 2001. We mainly analyze... We have studied 172 field-aligned currents (FACs) cases observed by the ClusterlI satellites when they crossed the plasma sheet boundary layer (PSBL) in the magnetotail from July to October 2001. We mainly analyzed the relationship between the characteristic of FACs at the PSBL in magnetotail and the Kp index. The main results indicated the followings: 1) In the different geomagnetic activity levels, the relative occurrence of FACs in PSBL increased monotonically with geomagnetic activity. 2) The density of FACs in PSBL increased monotonically with Kp index. In the storm main phase, the density of FACs increased dramatically, the maximum FACs approximately equaled 19.05 nA m-2 while Kp equaled 5.3) The variation of FACs density in PSBL was consistent with the variation of the Kp index. However, when AE〈800 nT, FACs density in PSBL increased with increasing AE, and when AE〉800 nT, it decreased with increasing AE. Therefore, our results suggested that the FACs density in PSBL had a closer correlation with Kp index. 展开更多
关键词 field-aligned currents plasma sheet boundary layer Kp index AE index
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