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含锌蛋白结构的电荷分布及蛋白质片段的结构优化
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作者 杨忠志 金宝鑫 赫兰兰 《辽宁师范大学学报(自然科学版)》 CAS 2016年第3期362-367,共6页
在PDB数据库中选取一系列的含锌蛋白模型分子并采用B3LYP/6-31+G*方法对氢原子进行部分优化,以Mulliken电荷为基准,利用线性回归和最小二乘法进行电荷分布计算,拟合出一套适用于含锌蛋白分子电荷计算的ABEEMσπ模型电荷参数.通过测试... 在PDB数据库中选取一系列的含锌蛋白模型分子并采用B3LYP/6-31+G*方法对氢原子进行部分优化,以Mulliken电荷为基准,利用线性回归和最小二乘法进行电荷分布计算,拟合出一套适用于含锌蛋白分子电荷计算的ABEEMσπ模型电荷参数.通过测试分子的检验,说明参数具有很好的可转移性.将拟合好的电荷参数融入ABEEMσπ浮动电荷极化力场,对3个蛋白质片段进行了力场优化并与PDB结构进行对比,得到相应键长和键角的偏差并统计了均方根偏差.键长均方根偏差最大值为0.005 6nm,键角均方根偏差最大值为3.75°.从优化结果来看,ABEEMσπ浮动电荷极化力场的参数是合理的,可以应用到更大的含锌蛋白体系中. 展开更多
关键词 含锌蛋白 模型分子 电荷分布 ABEEMσπ浮动电荷极化力场 可转移性
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同步辐射X荧光法测定电泳分离后蛋白条带内的锌 被引量:5
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作者 高愈希 丰伟悦 +4 位作者 李柏 章佩群 何伟 黄宇营 柴之芳 《核技术》 CAS CSCD 北大核心 2004年第3期165-168,共4页
本文初步建立了一种基于电泳分离和同步辐射X荧光(SRXRF)分析技术的生物样品内微量元素的种态分析方法。用同步辐射X荧光法直接分析了人肝细胞胞质溶胶等电聚焦(IEF)分离后蛋白条带内的金属锌含量。结果表明:肝细胞胞质溶胶内主要有三... 本文初步建立了一种基于电泳分离和同步辐射X荧光(SRXRF)分析技术的生物样品内微量元素的种态分析方法。用同步辐射X荧光法直接分析了人肝细胞胞质溶胶等电聚焦(IEF)分离后蛋白条带内的金属锌含量。结果表明:肝细胞胞质溶胶内主要有三个含锌条带,等电点(pI)分别为4.69、5.93、9.84。含锌量分别占总检出锌的33.08%、17.05%、26.54%。其中等电点为4.69的蛋白可能为金属硫蛋白。另外在等电点为6.60—9.30的范围内有若干含锌蛋白,共占总检出锌的23.33%。 展开更多
关键词 形态分析 含锌蛋白 同步辐射X荧光 等电聚焦 人肝细胞胞质溶胶
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2-苄基-3-硝基丙酸甲酯的水解条件 被引量:1
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作者 孟令强 王思宏 +2 位作者 葛东升 李运欣 田哲熙 《延边大学学报(自然科学版)》 CAS 2009年第1期55-57,共3页
探讨了含锌蛋白酶抑制剂——2-苄基-3-硝基丙酸的生成条件,即2-苄基-3-硝基丙酸甲酯的水解条件和在不同条件下副产物以及产物形成的可能机理.结果表明,以碘化锂作催化剂水解2-苄基-3-硝基丙酸甲酯能以较高的收率得到2-苄基-3-硝基丙酸.
关键词 含锌蛋白 抑制剂 2-苄基-3-硝基丙酸 碘化锂
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Effect of Copper, Zinc and Boron on Green Leaf Retention and Grain Yield of Winter and Spring Cereals
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作者 Syed Shah Sarah Hookway +3 位作者 Andrew Richards Carl Flint Sarah Wilkinson John Mark Fletcher 《Journal of Agricultural Science and Technology(B)》 2015年第6期365-375,共11页
Crop nutrition has a significant effect on disease incidence, resistance or tolerance of various crops. There is currently a lack of reliable recent UK-based research about the effect of copper, zinc and boron on dise... Crop nutrition has a significant effect on disease incidence, resistance or tolerance of various crops. There is currently a lack of reliable recent UK-based research about the effect of copper, zinc and boron on disease incidence, green leaf area (GLA), green leaf retention (GLR) and grain yield of winter wheat, spring wheat and spring barley. Data analysis showed that these trace elements had positive effects on GLR. These positive effects may have been due to the role of copper, zinc and boron in the production of defence related compounds (phenolics and lignin), which may have reduced the disease incidence resulting in prolonged GLR. Grain yield was significantly enhanced by the application of these trace elements on the crop grown on high pH calcareous soil, which can be partly attributed to enhanced GLR. Also trace elements have a positive role in reproductive growth, flowering and male fertility. On average, zinc was found to be the most consistent trace element in terms of enhancing GLR and grain yield. Across all trials, it was noted that for every 10% increase in GLA from trace elements, grain yields increased by 4.2% in 2012-2013, by 4.4% in 2013-2014 in winter wheat and by 3.9% in spring wheat in 2014. These are remarkably consistent and indicate that increasing GLA by 10% by early dough stage was associated with a yield improvement of about 4%. These trace elements also had a positive effect on grain protein content (GPC). This research concluded that the trace elements had positive effects in enhancing GLA and yield. It can be speculated that with the use of these trace elements, there may be more scope for using less robust or reduced rates of fungicides to control foliar diseases, which may help to maximize farm profits. 展开更多
关键词 Trace elements COPPER ZINC BORON CEREALS GLR grain yield.
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