吴茱萸中1个新的苯丙素苷类化合物

目的研究吴茱萸Euodia rutaecarpa的化学成分。方法采用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱等多种色谱手段进行分离纯化,利用波谱学方法鉴定化合物的结构。结果从吴茱萸75%乙醇提取物中分离得到8个化合物,分别鉴定为芥子醇9-O-阿魏酰基-4-O-β-D-葡萄糖苷(1)、3-O-阿魏酰基奎尼酸甲酯(2)、咖啡酸(3)、阿魏酸(4)、对羟基桂皮酸(5)、4-甲氧基苯甲醇(6)、3,4-二羟基苯甲酸(7)、7-羟基香豆素(8)。结论化合物1为1个新的苯丙素苷类化合物,命名为新吴茱萸苷。化合物2、3、6、7为首次从吴茱萸属植物中分离得到。

UFLC-PDA同时快速测定藏药翼首草中5种化学成分的含量

目的：建立同时快速测定藏药翼首草药材中绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷A含量的方法。方法：采用超快速液相色谱仪、Agilent Poroshell 120 SB-C18色谱柱（100 mm＆#215;4.6 mm，2.7μm），柱温30益，流速1.0 mL·min-1，进样量4μL，流动相为：乙腈-0.2%磷酸水溶液，梯度洗脱，检测波长237 nm、325 nm。结果：绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷 A分别在8.72-218.0、1.52-38.0、2.44-61.0、29.36-734.0、3.00-75.0μg·mL-1范围内（r〉.9996，n =6）呈良好线性关系，平均加样回收率分别为99.46%、99.41%、100.14%、98.89%、99.42%，RSD 分别为0.69%、0.66%、0.60%、1.21%、0.64%（n=9）。结论：该方法简便、准确、重复性好，可同时快速测定藏药翼首草中5种化学成分的含量。

Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD

A reversed-phase high-performance liquid chromatography （RP-HPLC） method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction, namely dehydroevodiamine, isorhanmetin-7-O- rutinoside, 10-hydroxyrutaecarpine, diosmetin-7-O-13-D-glucopyranoside, evodiamine, rutaecarpine, 1-metbyl-2-n-nonyl-4（1H） quinolone, evocarpine, and dihydroevocarpine. The RP-HPLC assay was performed on a reversed-phase Cl8 column with a gradient elution. The mobile phases consisted of methanol and water containing 1% （v/v） acetic acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm. All calibration curves showed good linearity （r2〉0.9941） within test ranges. The results of intra-day precision, inter-day precision, and accuracy were satisfactory. The overall recoveries were in the range of 90.13%-102.48%. The method was successfully applied to quantify nine bioactive compounds in six samples. Baseline resolution for all nine major compounds was achieved. This method could be used as a suitable quality control assay for Wuzhuyu decoction.