吸光分析中理论回归线质控应用初探

在盐酸副玫瑰苯胺比色法测定SO_2过程中,由分析方法确定显色物质的摩尔吸光系数(ε_(546)=4.77×10~4l·mol^1)和标准系列浓度,算出对应吸光值,用统计相关法求出浓度-吸光度回归线(理论线),以此为标准对实测标准曲线进行拟合检验,再用各实测标准曲线的系例浓度推算出吸光值与相应理论线吸光值进行比较,将各检验t值标在以浓度为横轴,t值为纵轴的质控图上。我们所做4条校正曲线,有2线拟合度差异不显著(P>0.50),t值均在警告限内,另外2线拟合度差异显著(P<0.01),t值随浓度增加逐次超出控制限。

紫外可见吸光光度分析

1、前言1.1 什么是吸光光度分析 吸光光度分析,是把样品中所要定量的成分转换成吸光物质(分子,离子),然后测定光被吸收以后的强度,以此来确定样品中该成分的浓度. 大概是历史上的原因,吸光光度分析的名称很多.有光吸收分析。

罗丹明类染料在分析化学中的应用进展

综述了 90年代以来 ,罗丹明类染料在吸光光度分析、荧光分析、动力学分析等方面的应用。利用罗丹明染料进行测定的物质达 50多种 ,且灵敏度很高。

有色金属矿石及其金属的分析进展——庆祝昆明冶金研究院分析室成立50周年

叙述了昆明冶金研究院分析室自1953年至2003年在滴定分析、吸光光度分析、极谱分析、原子吸收和原子发射光谱分析以及ICP-AES等分析方面取得的主要成绩。

光度分析中工作曲线偏离朗伯-比尔定律的原因

本文详细讨论了吸光光度分析中,引起工作曲线偏离朗伯—比尔定律的原因.分析得出在实验过程中,实验条件的选择、仪器性能及实验操作技术等均会引起实验误差.为提高分析准确度,本文提出了相应的解决办法.

新试剂N-邻甲苯基-N′-(氨基对苯磺酸钠)硫脲的合成及与金(Ⅲ)发色反应的研究和应用

详细介绍了新试剂N-邻甲苯基-N′-(氨基对苯磺酸钠)硫脲(OM PT)的合成。经过红外、紫外、核磁共振和元素分析等方法测试,确定了其组成和结构。并研究了试剂与金(Ⅲ)的发色反应,建立了光度法测定微量金(Ⅲ)的新方法。在pH4.2～5.6 的HAc～NaAc 缓冲体系中,该试剂和金(Ⅲ)形成一种稳定的组成比为 1∶2 的水溶性配合物,配合物的最大吸收峰位于 312.8nm 处,表观摩尔吸光系数为 1.74×105L·m ol- 1 ·cm - 1。Au3+ 量在 0.20～8.40μg/25m L 服从比尔定律,相关系数r= 0.9998。将该法应用于金矿石中微量金的测定,获得满意的结果。

Purification and Characterization of Flammulin,a Basic Protein with Anti-tumor Activities from Flammulina velutipes

Aim To purify and characterize flammulin, a basic protein with anti-tumoractivities. Methods Ammonium sulfate, ethanol fractionation and column chromatography were used forseparation and purification. Electrophoretic analysis, amino acid analysis, and MS of flammulin werecarried out. Results Flammulin was purified to electrophoretic homogeneity and crystallized. With amolecular mass of 19891.13 Da, pI 8.9, λ_(max) = 276 - 278 nm, λ_(min) = 250 nm, flammulin wascharacterized by its lack of methionine. Fingerprint mapping of flammulin was determined by MALDI-MSfollowing in-gel protease digestion; no close matches were identified. Conclusion Flammulin waspurified to electrophoretic homogeneity, and its characteristics are discussed for the first time.

Primary Speciation Analysis on 6 Kinds of Microelements in Glycyrrhiza uralensis Fisch.

[Objective]The aim was to analyze the primary speciation of 6 microelements in Glycyrrhiza uralensis Fisch. and provide theoretical basis for explaining pharmacodynamic principle of liquorice and discussing quality control of liquorice planting. [Method]The 6 elements Cu,Zn,Ca,Fe,Mg and Mn in roots of G.uralensis were extracted based on traditional decoction method and were separated into water-soluble state and suspension state by micro porous filtering film. The elements in water-soluble state were detected by flame atomic adsorption spectrophotometry （FAAS）. [Result]The results showed that extractive rates of the elements were in the range of 1.71%-60.06%,and immerse-residue ratio in 0.018 3-1.682 0; the results also indicated that the immerse-residue ratio of Zn was biggest （1.68）,Zn played an important medical role and might be considered as the best characteristic element in G.uralensis; the recoveries of the elements were ranged from 95.72% to 103.15% and relative standard deviations （RSD） were less than 2.38%. [Conclusion]Because of its high accuracy,FAAS method is feasible for analyzing primary speciation of microelements in G.uralensis.

塑料铅笔擦

1、适用范围本发明对用于擦试铅笔字迹的塑料擦(以下简称“铅笔擦”)作出规定.注(1)是指JIS S 6006标准规定的铅笔以及JIS S 6005标准规定的活动铅笔用芯,或同等以上的产品.备考 本文引用标准如下:JIS K 0050化学分析方法通则JIS K 0121 原子吸光分析通则JIS P 3201 书写用纸JIS P 6005 铅笔、色芯铅笔及活动铅笔用芯JIS S 6006 铅笔及色芯铅笔2、品质2.1外观 铅笔擦外形应匀整,无裂纹、气泡.混入异物及其它使用上的障碍,无污染,以及外观上显著损坏等缺点.

Science Letters:Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.

Effect of sodium pyrophosphate on the flotation separation of chalcopyrite from galena

The effect of sodium pyrophosphate （SPH） on the separation of chalcopyrite from galena was examined through flotation, adsorption, electrokinetic studies and infrared spectral analysis. Differential flotation tests indicate that satisfactory separation can be achieved within the pH range from 2.5 to 6 using SPH to depress the galena, but not the chalcopyrite when O-isopropyl-N-ethyl thionocarbamate （IPETC） is used as the collector. The electrophoretic mohilities of both the minerals dramatically become negatively charged following SPH adsorption in the pH range from 2.5 to 12, The infrared spectral analysis suggests that chemical adsorption occurs on galena surface treated by SPH, indicating that a chelate complex has formed. At weakly acidic pH values, the adsorption density of IPETC onto galena is significantly reduced in the presence of SPH. However, the amount of IPETC adsorbed onto chalcopyrite almost remains at the same level. Since the observed adsorption density of IEPTC onto chalcopyrite is quite high compared to galena, the observed flotation results are explained. A possible mechanism for the interaction between the two sulphide minerals and SPH is discussed.

Heavy metal content in coral reef sediments from Red Sea of Yemen and its significance on marine environment

In order to determine and assess the concentrations of trace elements in coral reefs sediments from Red Sea of Yemen, sediment samples were collected, treated and analyzed for cadmium, chromium, cobalt, copper, manganese, nickel, lead, iron, zinc and vanadium by the atomic absorption spectrometric analysis. The result is that cadmium, cobalt and lead concentrations were high and other elements are low or the same as natural background. It is concluded that the high cadmium, cobalt and lead levels in coral reefs sediments will have negative effects on marine life of the sites, so further researches are needed to characterize the sources fate, biogeochemical processes and impacts of these trace elements on coral reefs and marine of the region.

Improvement of scattering correction for in situ coastal and inland water absorption measurement using exponential fitting approach

The absorption coefficient of water is an important bio-optical parameter for water optics and water color remote sensing. However, scattering correction is essential to obtain accurate absorption coefficient values in situ using the nine-wavelength absorption and attenuation meter AC9. Establishing the correction always fails in Case 2 water when the correction assumes zero absorption in the near-infrared(NIR) region and underestimates the absorption coefficient in the red region, which affect processes such as semi-analytical remote sensing inversion. In this study, the scattering contribution was evaluated by an exponential fitting approach using AC9 measurements at seven wavelengths(412, 440, 488, 510, 532, 555, and 715 nm) and by applying scattering correction. The correction was applied to representative in situ data of moderately turbid coastal water, highly turbid coastal water, eutrophic inland water, and turbid inland water. The results suggest that the absorption levels in the red and NIR regions are significantly higher than those obtained using standard scattering error correction procedures. Knowledge of the deviation between this method and the commonly used scattering correction methods will facilitate the evaluation of the effect on satellite remote sensing of water constituents and general optical research using different scatteringcorrection methods.

Environmantal Risk Phytoaccumulation of Arsenic in Spinacea Olaracea L, and Lactuca Sativa L

Pollution of the environment, particularly the soil, appears to be one of the major contemporary issues. In addition, many studies point to the involvement of ecological risk of heavy metals into the food chain through edible plants like Spinaceae oleraceae L. and Lactuca sativa L.. This paper presents the results of the ＂ex-situ＂ application of phytoacumulation on the soils of contaminated terrain using the plant species of spinach （Spinacea oleracea L.） and lettuce （Lactuca sativa L.）. Sequestration of arsen from the soil by these plant species was observed through phytoaccumulation factor--PF and limit values （mg/kg）. The main task and objective of this research was to establish the level of accumulation of toxic element As from the soil by plants and to calculate the PF factor of transfer. The experiment was set up in the control conditions where the soil from eight contaminated locations was placed in the experimental containers. The AAS method was used to analyze heavy metal in plant material and soil. PF factor values ranged from 0.1 to 1.0 which indicates that the spinach and lettuce plants rechargeable moderate compared to arsenic.

Vibrational Spectra and Adsorption of Trisiloxane Superspreading Surfactant at Air/Water Interface Studied with Sum Frequency Generation Vibrational Spectroscopy

The C-H stretch vibrational spectra of the trisiloxane superspreading surfactant Silwet L-77 （（CH3）3Si- O-Si（CH3）（C3H6）（OCH2CH2）7-8OCH3）-O-Si（CH3）3） at the air/water interface are measured with the surface Sum Frequency Generation Vibrational Spectroscopy （SFG-VS）. The spectra are dominated with the features from the -Si-CH3 groups around 2905 cm^-1 （symmetric stretch or SS mode） and 2957 ^-1 （mostly the asymmetric stretch or AS mode）, and with the weak but apparent contribution from the -O-CH2- groups around 2880 ^-1 （symmetric stretch or SS mode）. Comparison of the polarization dependent SFG spectra below and above the critical aggregate or micelle concentration （CAC） indicates that the molecular orientation of the C-H related molecular groups remained unchanged at different surface densities of the Silwet L-77 surfactant. The SFG-VS adsorption isotherm suggested that there was no sign of Silwet L-77 bilayer structure formation at the air/water interface. The Gibbs adsorption free energy of the Silwet surfactant to the air/water interface is -42.2±0.8kcal/mol, indicating the unusually strong adsorption ability of the Silwet L-77 superspreading surfactant.

Cross-Validation of a Mobile N20 Flux Prototype （IPNOA） Using Micrometeorological and Chamber Methods

Within a LIFE＋ project IPNOA （improved flux prototype for n2o emission from agriculture）, LIFE11 ENV/IT/302 is a mobile prototype was developed to evaluate at field scale N20 emissions using a fast chamber technique. Main challenge was to develop a mobile system capable of moving on various field surfaces, equipped with very reliable N20 gas analyser （Los Gatos Research Inc.）, electrically autonomous （with batteries） and enough robust to face up to field conditions. In this paper, we report the major features of this prototype studied during two field campaigns. The N20 flux IPNOA prototype was compared with other methodological implementations： first, during an INGOS （integrated non-CO2 greenhouse gas observing systems） campaign on a grazed grassland at Easter Bush （Scotland） by Eddy correlation method, and then after on an arable crop at Grignon （France） using automatic and manual chambers fitted with QC-TILDAS （Quantum Cascade Tunable Infrared Laser Differential Absorption Spectrometer, Aerodyne Research Inc.）, with the 46C model of thermo-instrument analyser or with a GC （gas chromatography） analysis.

Study on the Interaction Between Tangerine Peel and Beer by Absorption Spectrum

This paper presents a new method to study the interaction between tangerine peel and beer by absorption spectrum.The author explores the change laws and the differences of the absorbance on some wave band from the absorption spectrums of tangerine peel and the mixture of tangerine peel and beer. The results show that there is an obvious difference around 323 nm though the absorbance values of the two samples are almost similar in the most bands. The absorbance value of the mixture is obviously greater than that of tangerine peel at 276 nm in the spectrums, and there is a shift of the peak position in the absorption spectrum of the mixture, which shows the differences of the absorbance values and the peak positions of the two samples. Through comparing the characteristics of the two samples' absorption spectrums, a new method for researching new pharmacological action of tangerine peel is presented in this paper. The result indicates that the technology of spectrum analysis will play an important role in the research and development of Chinese herbal pharmacology and new pharmacology.

Hygienic Quality of Fish Depending of Cadmium （Cd） and Lead （Pb） Content in Bottom Sediments of Water Reservoirs Kolinany

Analysis of bottom sediments in water reservoir Kolifiany from the aspect of Cd and Pb contents, the determination of observed heavy metal contents in different parts of carp body （meat muscle, liver and kidney） and the evaluation of hygienic status and suitability of fish meat for the human consumption were performed. For analysis, each tissue sample was dissolved in a solution of nitric acid p.a. （HNO3：HzO = 2：1）; sediment sample in solution of acids （HF-HNO3-HCIO4） and analyzed for presence of Cd, Pb by the atomic absorption spectrophotometer （AAS） Varian Duo 240FS and 240Z. Our results confirmed hygienic harmless of bottom sediments in water reservoir Kolifiany. The Cd, Pb contents in sediments represent no risk within their input into the fish organisms. The Cd content in fish meat is lower than maximum available amount given by legislative norms, and the contents of Pb in fish meat too. The results of Cd, Pb content determination in carp body confirmed that fish muscles belong to suitable foodstuff for the human consumption.