报道鸭盲肠杯叶吸虫的生活史，证实纹沼螺 Paraf ossarulus striatulus 和泥鳅 Misgurnus auguillicaudatus 是该吸虫的第一、二中间宿主。虫卵在20～28℃和适当光照条件下21 d 孵出毛蚴；毛蚴侵入纹沼螺后发育为胞蚴和尾蚴，并从62 d ...报道鸭盲肠杯叶吸虫的生活史，证实纹沼螺 Paraf ossarulus striatulus 和泥鳅 Misgurnus auguillicaudatus 是该吸虫的第一、二中间宿主。虫卵在20～28℃和适当光照条件下21 d 孵出毛蚴；毛蚴侵入纹沼螺后发育为胞蚴和尾蚴，并从62 d 开始自螺体向外逸出尾蚴；尾蚴侵入泥鳅体表和肌肉内经10 d 结成囊蚴；家鸭口服囊蚴后3 d ，囊蚴在鸭盲肠内发育为成虫并向外排卵。整个发育周期持续96 d 。展开更多
A 40-day gnotobiotic microcosm experiment was carried out to quantify the effect of hastenal-feeding nematode on plant growth and nutrient absorption. The results showed that inoculation of bacterial-feeding nematode ...A 40-day gnotobiotic microcosm experiment was carried out to quantify the effect of hastenal-feeding nematode on plant growth and nutrient absorption. The results showed that inoculation of bacterial-feeding nematode Protorhabditis sp. stimulated the growth of wheat (Triticum aestivum) and the uptake of N. By the end of the 40-day incubation wheat biomass and N uptake in the treatment with nematode and bacteria (Pseudomonas sp.) increased by 6.5% and 5.9%, respectively, compared with bacteria alone treatment. The presence of nematode mainly accelerated the growth of aboveground of wheat, while it slightly inhibited the root development. There was little difference in plant tissue N concentration between treatments. P concentration and uptake of wheat, however, were generally reduced by nematode. It appears that the enhancement of plant growth and nitrogen uptake is attributed to the enhancement of nitrogen mineralization induced by nematode feeding on bacteria, and the reduction of phosphorous uptake is the result of weak root status and competition by bacteria immobilization.展开更多
Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from...Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .展开更多
Studies on the prevalence and pathology of Fasciola specie were carried out in nine abattoirs in Ilorin, capital city of Kwara State, Nigeria. The research was conducted during the period August, 2006 - May, 2007. Of ...Studies on the prevalence and pathology of Fasciola specie were carried out in nine abattoirs in Ilorin, capital city of Kwara State, Nigeria. The research was conducted during the period August, 2006 - May, 2007. Of the 63,649 cattles examined at the various abattoirs which are lpata, Sango, Oloje, Baboko, Oke-oyi, Iporin, Panada, Oke-ose and Marafa, 1,434 representing 25.2% were infected with Fasciola species while 114 representing 6.5% were condemned. Iporin slaughter slab had the highest prevalence of 3.6%, followed by panada with 3.4% and Ipata having the least prevalence of 1.6%. Similarly analysis of data on monthly basis showed that March had the highest prevalence of 2.8%, followed by April with 2.6% and December with the least prevalence of 1.8%. Overall infestation was 22.7% while overall condemnation was 6.5%. There was a significant difference （X^2 = 23.7, P 〈 0.05） in the monthly infestation rate. Also, there was no significant difference （X^2 = 10.7, P 〉 0.05） between the infection rate and condemnation among the various abattoirs. The factors responsible for the prevalence of Fasciola specie and pathology of infection are highlighted.展开更多
文摘报道鸭盲肠杯叶吸虫的生活史，证实纹沼螺 Paraf ossarulus striatulus 和泥鳅 Misgurnus auguillicaudatus 是该吸虫的第一、二中间宿主。虫卵在20～28℃和适当光照条件下21 d 孵出毛蚴；毛蚴侵入纹沼螺后发育为胞蚴和尾蚴，并从62 d 开始自螺体向外逸出尾蚴；尾蚴侵入泥鳅体表和肌肉内经10 d 结成囊蚴；家鸭口服囊蚴后3 d ，囊蚴在鸭盲肠内发育为成虫并向外排卵。整个发育周期持续96 d 。
基金Project (No. 39570134 and 39970419) supported by the National Natural Science Foundation of China.
文摘A 40-day gnotobiotic microcosm experiment was carried out to quantify the effect of hastenal-feeding nematode on plant growth and nutrient absorption. The results showed that inoculation of bacterial-feeding nematode Protorhabditis sp. stimulated the growth of wheat (Triticum aestivum) and the uptake of N. By the end of the 40-day incubation wheat biomass and N uptake in the treatment with nematode and bacteria (Pseudomonas sp.) increased by 6.5% and 5.9%, respectively, compared with bacteria alone treatment. The presence of nematode mainly accelerated the growth of aboveground of wheat, while it slightly inhibited the root development. There was little difference in plant tissue N concentration between treatments. P concentration and uptake of wheat, however, were generally reduced by nematode. It appears that the enhancement of plant growth and nitrogen uptake is attributed to the enhancement of nitrogen mineralization induced by nematode feeding on bacteria, and the reduction of phosphorous uptake is the result of weak root status and competition by bacteria immobilization.
文摘Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .
文摘Studies on the prevalence and pathology of Fasciola specie were carried out in nine abattoirs in Ilorin, capital city of Kwara State, Nigeria. The research was conducted during the period August, 2006 - May, 2007. Of the 63,649 cattles examined at the various abattoirs which are lpata, Sango, Oloje, Baboko, Oke-oyi, Iporin, Panada, Oke-ose and Marafa, 1,434 representing 25.2% were infected with Fasciola species while 114 representing 6.5% were condemned. Iporin slaughter slab had the highest prevalence of 3.6%, followed by panada with 3.4% and Ipata having the least prevalence of 1.6%. Similarly analysis of data on monthly basis showed that March had the highest prevalence of 2.8%, followed by April with 2.6% and December with the least prevalence of 1.8%. Overall infestation was 22.7% while overall condemnation was 6.5%. There was a significant difference （X^2 = 23.7, P 〈 0.05） in the monthly infestation rate. Also, there was no significant difference （X^2 = 10.7, P 〉 0.05） between the infection rate and condemnation among the various abattoirs. The factors responsible for the prevalence of Fasciola specie and pathology of infection are highlighted.
