Objective To investigate if immunological factors associated with rheumatoid arthritis(RA) affect the result of human immunodeficiency virus(HIV) screening by electrochemiluminescence immunoassay(ECLIA) and enzyme-lin...Objective To investigate if immunological factors associated with rheumatoid arthritis(RA) affect the result of human immunodeficiency virus(HIV) screening by electrochemiluminescence immunoassay(ECLIA) and enzyme-linked immunosorbent assay(ELISA). Methods 100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors(RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA. Results The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method(P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA(P<0.01). Conclusion Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.展开更多
Chromium is a toxic heavy metal that is widely used in industries such as metallurgical industries, and tannery industries. This study investigates the use of an alternative adsorption technology, biosorption, using d...Chromium is a toxic heavy metal that is widely used in industries such as metallurgical industries, and tannery industries. This study investigates the use of an alternative adsorption technology, biosorption, using dried biomass of water spinach （Ipomoea aquatica） in removing chromium from aqueous solutions. Dried biomass was prepared through washing, drying, cutting and alkali treatment. K,Cr207 solutions at an initial concentration of 200~ 14.72 mg/L were added to biomass weight of 200.0~ 1.1 mg at varying pH values of 1.00, 2.00, 3.00~0.02, 4.00~0.58 and 5.00~0.44. The maximum total chromium ion uptake was at pH=2, wherein the biomass adsorbed was 9.56~0.64 ppm Cr/mg biomass. K2Cr207 solutions at concentrations of 10.00~0.32, 50.00~0.95, 100.00~0.76, 200.00~4.82 and 300.00~4.69 mg/L were added to flasks with a biomass weight of 200.00~0.75 mg and pH of 2.00~0.25. The behavior of chromium ion biosorption unto the dried water spinach best fits the Langmuir isotherm with a coefficient of determination （R2） value of 0.9993. The calculated maximum adsorption （%） is 0.13889 kg/kg. The adsorption equilibrium constant （K） is 1705.83 mg/L. The continuous experiment showed that the kinetic behavior of the biomass in a packed bed column followed the following equation with a coefficient of determination （R2） value of 0.7039： C/C0=0.266e^-0.004t展开更多
以Van Der Waals力作用半径为边界条件,构建了煤大分子骨架模型及煤对CH4吸附的量子动力学模型;采用量子化学DFT方法,在6-311G++基组上对煤大分子骨架模型及煤对CH4分子吸附模型结构进行了优化,得到了多种吸附平衡态的几何构型以及吸附...以Van Der Waals力作用半径为边界条件,构建了煤大分子骨架模型及煤对CH4吸附的量子动力学模型;采用量子化学DFT方法,在6-311G++基组上对煤大分子骨架模型及煤对CH4分子吸附模型结构进行了优化,得到了多种吸附平衡态的几何构型以及吸附的稳定平衡结构;通过煤大分子骨架模型的Mulliken Atomic Charges分析,研究了煤分子对CH4分子的吸附中心,吸附位能曲线,以及CH4分子键长键角的变化。展开更多
基金Supported by Shanghai Municipal Natural Science Foundation(11ZR1427000)
文摘Objective To investigate if immunological factors associated with rheumatoid arthritis(RA) affect the result of human immunodeficiency virus(HIV) screening by electrochemiluminescence immunoassay(ECLIA) and enzyme-linked immunosorbent assay(ELISA). Methods 100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors(RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA. Results The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method(P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA(P<0.01). Conclusion Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.
文摘Chromium is a toxic heavy metal that is widely used in industries such as metallurgical industries, and tannery industries. This study investigates the use of an alternative adsorption technology, biosorption, using dried biomass of water spinach （Ipomoea aquatica） in removing chromium from aqueous solutions. Dried biomass was prepared through washing, drying, cutting and alkali treatment. K,Cr207 solutions at an initial concentration of 200~ 14.72 mg/L were added to biomass weight of 200.0~ 1.1 mg at varying pH values of 1.00, 2.00, 3.00~0.02, 4.00~0.58 and 5.00~0.44. The maximum total chromium ion uptake was at pH=2, wherein the biomass adsorbed was 9.56~0.64 ppm Cr/mg biomass. K2Cr207 solutions at concentrations of 10.00~0.32, 50.00~0.95, 100.00~0.76, 200.00~4.82 and 300.00~4.69 mg/L were added to flasks with a biomass weight of 200.00~0.75 mg and pH of 2.00~0.25. The behavior of chromium ion biosorption unto the dried water spinach best fits the Langmuir isotherm with a coefficient of determination （R2） value of 0.9993. The calculated maximum adsorption （%） is 0.13889 kg/kg. The adsorption equilibrium constant （K） is 1705.83 mg/L. The continuous experiment showed that the kinetic behavior of the biomass in a packed bed column followed the following equation with a coefficient of determination （R2） value of 0.7039： C/C0=0.266e^-0.004t
文摘以Van Der Waals力作用半径为边界条件,构建了煤大分子骨架模型及煤对CH4吸附的量子动力学模型;采用量子化学DFT方法,在6-311G++基组上对煤大分子骨架模型及煤对CH4分子吸附模型结构进行了优化,得到了多种吸附平衡态的几何构型以及吸附的稳定平衡结构;通过煤大分子骨架模型的Mulliken Atomic Charges分析,研究了煤分子对CH4分子的吸附中心,吸附位能曲线,以及CH4分子键长键角的变化。
