AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time ...AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.展开更多
Fibers are used in various areas for improving the performance of different materials, commonly used are synthetic fibers and glass fibers. More and more sustainable alternatives are required to reduce energy consumpt...Fibers are used in various areas for improving the performance of different materials, commonly used are synthetic fibers and glass fibers. More and more sustainable alternatives are required to reduce energy consumption and the carbon footprint. Traditional natural fibers (like hemp or flax) very often do not fulfill requirements for construction purposes like resistance to elevated temperature or lacking purity. Also mechanical properties of natural fibers are influenced by factors like harvesting, kink bands, climate and growth conditions. Lenzing AG has put a lot of efforts into developing a sustainable fiber overcoming the above mentioned issues. The raw material for TENCEL is wood, which is transformed into a fiber of pure cellulose in an economy friendly process as been proven by a life cycle assessment. The properties of a composite material are highly dependent on parameters like mechanical fiber properties, fiber diameter, quality of fiber dispersion and fiber matrix adhesion. Keeping these properties constant throughout the whole composite part is the factor to success. The diameter as well as the mechanical properties of TENCEL fibers is kept within a very narrow range thanks to the unique manufacturing process. It was shown that the fiber dispersion of TENCEL as well as the fiber matrix adhesion is better than for natural fibers.展开更多
In this paper, for a sustainable building in a Mediterranean climate areas, the relation between envelope and energy is discussed. The relation between envelopes and energy has been considered in all the phases of the...In this paper, for a sustainable building in a Mediterranean climate areas, the relation between envelope and energy is discussed. The relation between envelopes and energy has been considered in all the phases of the life building (construction phase and during operation). This has been made through global assessment of massive envelopes (with or without insulation) realized with blocks of different materials: natural stone, concrete and brick. For these types of envelope the thermal comfort of the rooms, the energy requirement for cooling and heating, economic and environmental aspects (costs and embodied energy) in the life cycle of the building have been studied.展开更多
The appropriate production of liquefied natural gas(LNG)with least consuming energy and maximum efficiency is quite important.In this paper,LNG production cycle by means of APCI Process has been studied.Energy equilib...The appropriate production of liquefied natural gas(LNG)with least consuming energy and maximum efficiency is quite important.In this paper,LNG production cycle by means of APCI Process has been studied.Energy equilibrium equations and exergy equilibrium equations of each equipment in the APCI cycle were established.The equipments are described using rigorous thermodynamics and no significant simplification is assumed.Taken some operating parameters as key parameters,influences of these parameters on coefficient of performance(COP)and exergy efficiency of the cascading cycle were analyzed.The results indicate that COP and exergy efficiency will be improved with the increasing of the inlet pressure of MR(mixed refrigerant)compressors,the decreasing of the NG and MR after precooling process,outlet pressure of turbine,inlet temperature of MR compressor and NG temperature after cooling in main cryogenic heat exchanger(MCHE).The COP and exergy efficiency of the APCI cycle will be above 2% and 40%,respectively,after optimizing the key parameters.展开更多
基金Supported by the Indian Council of Medical Research(ICMR)intramural projectDas C acknowledges the financial support from Biomolecular Assembly,Recognition and Dynamics (BARD) project(Grant 12-R&D-SIN-5.04-0103)from Department of Atomic Energy(DAE),Government of India and Ramalingaswami Fellowship,Department of Biotechnology,Government of India
文摘AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.
文摘Fibers are used in various areas for improving the performance of different materials, commonly used are synthetic fibers and glass fibers. More and more sustainable alternatives are required to reduce energy consumption and the carbon footprint. Traditional natural fibers (like hemp or flax) very often do not fulfill requirements for construction purposes like resistance to elevated temperature or lacking purity. Also mechanical properties of natural fibers are influenced by factors like harvesting, kink bands, climate and growth conditions. Lenzing AG has put a lot of efforts into developing a sustainable fiber overcoming the above mentioned issues. The raw material for TENCEL is wood, which is transformed into a fiber of pure cellulose in an economy friendly process as been proven by a life cycle assessment. The properties of a composite material are highly dependent on parameters like mechanical fiber properties, fiber diameter, quality of fiber dispersion and fiber matrix adhesion. Keeping these properties constant throughout the whole composite part is the factor to success. The diameter as well as the mechanical properties of TENCEL fibers is kept within a very narrow range thanks to the unique manufacturing process. It was shown that the fiber dispersion of TENCEL as well as the fiber matrix adhesion is better than for natural fibers.
文摘In this paper, for a sustainable building in a Mediterranean climate areas, the relation between envelope and energy is discussed. The relation between envelopes and energy has been considered in all the phases of the life building (construction phase and during operation). This has been made through global assessment of massive envelopes (with or without insulation) realized with blocks of different materials: natural stone, concrete and brick. For these types of envelope the thermal comfort of the rooms, the energy requirement for cooling and heating, economic and environmental aspects (costs and embodied energy) in the life cycle of the building have been studied.
文摘The appropriate production of liquefied natural gas(LNG)with least consuming energy and maximum efficiency is quite important.In this paper,LNG production cycle by means of APCI Process has been studied.Energy equilibrium equations and exergy equilibrium equations of each equipment in the APCI cycle were established.The equipments are described using rigorous thermodynamics and no significant simplification is assumed.Taken some operating parameters as key parameters,influences of these parameters on coefficient of performance(COP)and exergy efficiency of the cascading cycle were analyzed.The results indicate that COP and exergy efficiency will be improved with the increasing of the inlet pressure of MR(mixed refrigerant)compressors,the decreasing of the NG and MR after precooling process,outlet pressure of turbine,inlet temperature of MR compressor and NG temperature after cooling in main cryogenic heat exchanger(MCHE).The COP and exergy efficiency of the APCI cycle will be above 2% and 40%,respectively,after optimizing the key parameters.