作为密码应用核心要素,真随机数发挥着不可替代的作用.为保证其质量,真随机数大多基于随机物理现象构造的熵源产生,这也使得其易遭受由环境引入或攻击者恶意施加的扰动影响,进而对密码应用安全产生威胁.为确保真随机数的质量,当前各主...作为密码应用核心要素,真随机数发挥着不可替代的作用.为保证其质量,真随机数大多基于随机物理现象构造的熵源产生,这也使得其易遭受由环境引入或攻击者恶意施加的扰动影响,进而对密码应用安全产生威胁.为确保真随机数的质量,当前各主要国际标准均明确要求真随机数发生器(True Random Number Generator,TRNG)应针对熵源生成的原始随机数(raw random numbers)提供在线监测功能.然而,由于现有在线监测大多基于抽样统计方法构建,导致其在实际应用中存在实现复杂、耗费资源大等问题;另一方面,由于统计模型偏差及熵源输出分布受扰动因素影响等原因,使得现有在线监测方法普遍存在过估计问题.本文以当前广泛采用的振荡环熵源为对象,对扰动场景下熵源特性变异成因及影响进行了深入分析,提出了用于刻画熵源特性的异源同构周期计数差表征方法,并结合变异阈值的标定,构建了具备高准确度、强实时性的熵源在线监测方法.与当前各主要在线监测方法相比,该方法在资源耗费方面具有明显优势,能够实时反映扰动场景下熵源在噪声分量、扰动感应耦合等方面的变异情况,实现对TRNG健康特性的有效监测.展开更多
To set up a three-parameter method for cell cycle analysis by two-laser flowcy-tometer, which can detect two types of cyclin plus DNA content in one measurement, and thatanalyze unscheduled expression of cyclins. Meth...To set up a three-parameter method for cell cycle analysis by two-laser flowcy-tometer, which can detect two types of cyclin plus DNA content in one measurement, and thatanalyze unscheduled expression of cyclins. Methods: Three-color fluorescence was used for analysisof two types of cyclins and DNA content simultaneously in individual cells by two-laser flowcytometry. MOLT-4 cells were used to study the expression of major cyclins in mammalian cells. ATriton-X100 permeabilization procedure was optimized for detection of two types of cyclins. Onecyclin was stained directly with a FITC-conjugated monoclonal antibody (mAb), and the other,indirectly with RPE-Cy5-conjugated secondary antibody, while DNA was stained with the fluorochromeDAPI. mAMSA and mimosine treated MOLT-4 cells were used to test this three-parameter method.Results: Permeabilization with 0.5% Triton-XlOO in PBS containing 1% BSA for 5 min on ice providedoptimal conditions for the simultaneous labelling of two cyclins plus DNA in single cells. It wasfound that the emission spectrum of the three dyes (DAPI, FITC and RPE-Cy5) could be measured withno compensation. Based on cyclinA/cyclinE/DNA flow cytometric analysis, asynchronously growingMOLT-4 cells could be divided into 6 compartments (G1o, G1e, G1l, S, G2, and M) simultaneously,allowing for analysis of cell cycle phase specific perturbations without the necessity of cellsynchronization. Unscheduled cyclin B1 expression was observed in G1 cells treated with mimosine andcyclin E in G2 cells treated with mAMSA. We found that unscheduled cyclin expression paralleledexpected cyclin expression. Conclusion: Thus, three-color FCM analysis of cells may not only beapplied to measure unscheduled vs. expected cyclin expression but may also be used to estimate thefraction of cycling cells in up to 6 cell populations.展开更多
It is desired to obtain the joint probability distribution(JPD) over a set of random variables with local data, so as to avoid the hard work to collect statistical data in the scale of all variables. A lot of work has...It is desired to obtain the joint probability distribution(JPD) over a set of random variables with local data, so as to avoid the hard work to collect statistical data in the scale of all variables. A lot of work has been done when all variables are in a known directed acyclic graph(DAG). However, steady directed cyclic graphs(DCGs) may be involved when we simply combine modules containing local data together, where a module is composed of a child variable and its parent variables. So far, the physical and statistical meaning of steady DCGs remain unclear and unsolved. This paper illustrates the physical and statistical meaning of steady DCGs, and presents a method to calculate the JPD with local data, given that all variables are in a known single-valued Dynamic Uncertain Causality Graph(S-DUCG), and thus defines a new Bayesian Network with steady DCGs. The so-called single-valued means that only the causes of the true state of a variable are specified, while the false state is the complement of the true state.展开更多
The previous methods of figuring the numbers of chemical elements is summed up in this paper. Based on that, another two creative calculative methods are introduced as well.
Objective: To study the cellular immunity function of patientswith early syphilis and the effects on immune modifiersEsberitox N or IFN. Methods: T-lymphocyte subpopulations of the peripheralblood in 44 patients with ...Objective: To study the cellular immunity function of patientswith early syphilis and the effects on immune modifiersEsberitox N or IFN. Methods: T-lymphocyte subpopulations of the peripheralblood in 44 patients with syphilis and 40 healthy controls wereexamined by flow cytometry. Results: The number of CD_4+ cells and the CD_4+/CD_8+ ratioin patients with syphilis were found to be significantly lowerthan those in the control (P<0.0l), while the number of CD_8+cells was higher than that in the control (P<0.01). TheCD_4+/CD_8+ ratio in those with active disease was lower thanthat in those who had been cured (P<0.05). The CD_4+ countand the CD_4+/CD_8+ ratio in those treated with antibiotics alone(Penicillin G or Cephalosporins) were lower than those treatedwith both antibiotics and immunomodulators (P<0.05). Conclusions: Cellular immunity in the patients with earlysyphilis was prominently suppressed, and treatment withimmunomodulators may be helpful for the recovery of cellularimmunity of these patients.展开更多
It is common to assume that structures are designed in view of 50 year life cycle as per Euro-Code 2 and other codes. In special cases, structures are designed in view of longer life cycle, such as bridges, important ...It is common to assume that structures are designed in view of 50 year life cycle as per Euro-Code 2 and other codes. In special cases, structures are designed in view of longer life cycle, such as bridges, important infrastructure facilities, important religious structures or in case of extended returning period of seismic event or floods. Beside issues of durability and maintenance aspects, this involves also the need to cover the probability of exceeding characteristic design live loads during the extended period, while keeping the same levels of the accepted risk that were assumed by the various codes, as good enough for the standard 50 year life cycle. Bearing in mind that design procedures, formulations, materials characteristic strengths and partial safety factors are used for these structures as per the existing codes, scaling of partial safety factors, or alternatively an additional ＂compensating＂ factor is required. A simplified approach and procedure to arrive at a reasonable calibration of the code safety factors based on 50 years to compensate for an extended life cycle, based upon structural reliability considerations, is proposed.展开更多
A stochastic model is developed to predict the peniodic operation performance ofthe continuous counter-current adsorption process. The model takes into account theeffects of random backmixing of particles, axial dispe...A stochastic model is developed to predict the peniodic operation performance ofthe continuous counter-current adsorption process. The model takes into account theeffects of random backmixing of particles, axial dispersion of liquid phase, liquid- film mass transfer, intraparticle diffusion and panticle shape, and can revealclearly the behavior of solid and liquid phase in adsorption process. The simulation results agree with the experimental data rather well.展开更多
文摘作为密码应用核心要素,真随机数发挥着不可替代的作用.为保证其质量,真随机数大多基于随机物理现象构造的熵源产生,这也使得其易遭受由环境引入或攻击者恶意施加的扰动影响,进而对密码应用安全产生威胁.为确保真随机数的质量,当前各主要国际标准均明确要求真随机数发生器(True Random Number Generator,TRNG)应针对熵源生成的原始随机数(raw random numbers)提供在线监测功能.然而,由于现有在线监测大多基于抽样统计方法构建,导致其在实际应用中存在实现复杂、耗费资源大等问题;另一方面,由于统计模型偏差及熵源输出分布受扰动因素影响等原因,使得现有在线监测方法普遍存在过估计问题.本文以当前广泛采用的振荡环熵源为对象,对扰动场景下熵源特性变异成因及影响进行了深入分析,提出了用于刻画熵源特性的异源同构周期计数差表征方法,并结合变异阈值的标定,构建了具备高准确度、强实时性的熵源在线监测方法.与当前各主要在线监测方法相比,该方法在资源耗费方面具有明显优势,能够实时反映扰动场景下熵源在噪声分量、扰动感应耦合等方面的变异情况,实现对TRNG健康特性的有效监测.
基金This project was supported by grants from China Key Basic Research Program Grant (No. G1998051212) the National Natural Sciences Foundation of China (No. 39670265, 39730270 and 39725027) grants from the Science Foundation of Ministry of Public Health, China (No. 202-01-06).
文摘To set up a three-parameter method for cell cycle analysis by two-laser flowcy-tometer, which can detect two types of cyclin plus DNA content in one measurement, and thatanalyze unscheduled expression of cyclins. Methods: Three-color fluorescence was used for analysisof two types of cyclins and DNA content simultaneously in individual cells by two-laser flowcytometry. MOLT-4 cells were used to study the expression of major cyclins in mammalian cells. ATriton-X100 permeabilization procedure was optimized for detection of two types of cyclins. Onecyclin was stained directly with a FITC-conjugated monoclonal antibody (mAb), and the other,indirectly with RPE-Cy5-conjugated secondary antibody, while DNA was stained with the fluorochromeDAPI. mAMSA and mimosine treated MOLT-4 cells were used to test this three-parameter method.Results: Permeabilization with 0.5% Triton-XlOO in PBS containing 1% BSA for 5 min on ice providedoptimal conditions for the simultaneous labelling of two cyclins plus DNA in single cells. It wasfound that the emission spectrum of the three dyes (DAPI, FITC and RPE-Cy5) could be measured withno compensation. Based on cyclinA/cyclinE/DNA flow cytometric analysis, asynchronously growingMOLT-4 cells could be divided into 6 compartments (G1o, G1e, G1l, S, G2, and M) simultaneously,allowing for analysis of cell cycle phase specific perturbations without the necessity of cellsynchronization. Unscheduled cyclin B1 expression was observed in G1 cells treated with mimosine andcyclin E in G2 cells treated with mAMSA. We found that unscheduled cyclin expression paralleledexpected cyclin expression. Conclusion: Thus, three-color FCM analysis of cells may not only beapplied to measure unscheduled vs. expected cyclin expression but may also be used to estimate thefraction of cycling cells in up to 6 cell populations.
基金supported by the National Natural Science Foundation of China under Grant 71671103
文摘It is desired to obtain the joint probability distribution(JPD) over a set of random variables with local data, so as to avoid the hard work to collect statistical data in the scale of all variables. A lot of work has been done when all variables are in a known directed acyclic graph(DAG). However, steady directed cyclic graphs(DCGs) may be involved when we simply combine modules containing local data together, where a module is composed of a child variable and its parent variables. So far, the physical and statistical meaning of steady DCGs remain unclear and unsolved. This paper illustrates the physical and statistical meaning of steady DCGs, and presents a method to calculate the JPD with local data, given that all variables are in a known single-valued Dynamic Uncertain Causality Graph(S-DUCG), and thus defines a new Bayesian Network with steady DCGs. The so-called single-valued means that only the causes of the true state of a variable are specified, while the false state is the complement of the true state.
基金This work is supported by the National Science Foundation of China （No. 20471001 and 20671001）, the Important Project of Anhui Provincial Education Department （No. ZD2007004-1）, the Specific Project for Talents of Science and Technology of Universities of Anhui Province （No. 2005hbz03） and the Key Laboratory of Environment-friendly Polymer Materials of Anhui Province.
文摘The previous methods of figuring the numbers of chemical elements is summed up in this paper. Based on that, another two creative calculative methods are introduced as well.
文摘Objective: To study the cellular immunity function of patientswith early syphilis and the effects on immune modifiersEsberitox N or IFN. Methods: T-lymphocyte subpopulations of the peripheralblood in 44 patients with syphilis and 40 healthy controls wereexamined by flow cytometry. Results: The number of CD_4+ cells and the CD_4+/CD_8+ ratioin patients with syphilis were found to be significantly lowerthan those in the control (P<0.0l), while the number of CD_8+cells was higher than that in the control (P<0.01). TheCD_4+/CD_8+ ratio in those with active disease was lower thanthat in those who had been cured (P<0.05). The CD_4+ countand the CD_4+/CD_8+ ratio in those treated with antibiotics alone(Penicillin G or Cephalosporins) were lower than those treatedwith both antibiotics and immunomodulators (P<0.05). Conclusions: Cellular immunity in the patients with earlysyphilis was prominently suppressed, and treatment withimmunomodulators may be helpful for the recovery of cellularimmunity of these patients.
文摘It is common to assume that structures are designed in view of 50 year life cycle as per Euro-Code 2 and other codes. In special cases, structures are designed in view of longer life cycle, such as bridges, important infrastructure facilities, important religious structures or in case of extended returning period of seismic event or floods. Beside issues of durability and maintenance aspects, this involves also the need to cover the probability of exceeding characteristic design live loads during the extended period, while keeping the same levels of the accepted risk that were assumed by the various codes, as good enough for the standard 50 year life cycle. Bearing in mind that design procedures, formulations, materials characteristic strengths and partial safety factors are used for these structures as per the existing codes, scaling of partial safety factors, or alternatively an additional ＂compensating＂ factor is required. A simplified approach and procedure to arrive at a reasonable calibration of the code safety factors based on 50 years to compensate for an extended life cycle, based upon structural reliability considerations, is proposed.
文摘A stochastic model is developed to predict the peniodic operation performance ofthe continuous counter-current adsorption process. The model takes into account theeffects of random backmixing of particles, axial dispersion of liquid phase, liquid- film mass transfer, intraparticle diffusion and panticle shape, and can revealclearly the behavior of solid and liquid phase in adsorption process. The simulation results agree with the experimental data rather well.
