Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological...Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle.展开更多
The fatigue test between 10^5—10^9 cycles of GH4169 nickel‑based superalloy commonly used in aircraft engines is carried out by ultrasonic fatigue machine at 650℃.The S‑N curve is obtained and the fatigue fracture m...The fatigue test between 10^5—10^9 cycles of GH4169 nickel‑based superalloy commonly used in aircraft engines is carried out by ultrasonic fatigue machine at 650℃.The S‑N curve is obtained and the fatigue fracture morphology is observed.The fatigue S‑N curve presents a“step‑like”shape,with the first inflection point near 1×10^7 cycles and the second inflection point near 1×10^8 cycles.There is no engineering fatigue limit,and it still shows a downward trend after 107 or even 10^9 cycles.The crack initiation location is related to its life.Cracks are generated on the surface below 10^7 cycles,while it is inside above 10^7 cycles.The crack initiation source in the ultra‑high cycle fatigue at 650℃ is mainly the local intergranular fracture and casting defect of the matrix.In the phase of crack propagation,the mixed propagation of intergranular and cleavage is the main form.展开更多
The effects of inclusions in powder superalloy FGH96 on low-cycle fatigue life were studied, and a low-cycle crack initiation life prediction model based on the theory of damage mechanics was proposed. The damage char...The effects of inclusions in powder superalloy FGH96 on low-cycle fatigue life were studied, and a low-cycle crack initiation life prediction model based on the theory of damage mechanics was proposed. The damage characterization parameter was proposed after the construction of damage evolution equations. Fatigue tests of the powder superalloy specimens with and without inclusion were conducted at 530 and 600 ℃, and the model verification was carried out for specimens with elliptical, semi-elliptical, polygon and strip-shaped surface/subsurface inclusion. The stress analysis was performed by finite element simulation and the predicted life was calculated. The results showed a satisfying agreement between predicted and experimental life.展开更多
Low-cycle fatigue crack initiation behavior of nickel-based single crystal superalloy at 530℃ was investigated.Results show that the behavior of crack initiation is closely related to the maximum strain.When the maxi...Low-cycle fatigue crack initiation behavior of nickel-based single crystal superalloy at 530℃ was investigated.Results show that the behavior of crack initiation is closely related to the maximum strain.When the maximum strain is 2.0%,the fatigue crack is originated at the position of persistent slip bands on the surface of specimen,which is located on the{111}slip plane.No defects are observed at the crack initiation position.When the maximum strain is lower than 1.6%,the cracks are initiated at the casting defects on sub-surface or at interior of the specimen.The casting defects are located on the{100}slip plane vertical to the axial force.The crack is initiated along the{100}slip plane and then expanded along different{111}slip planes after a short stage of expansion.As the maximum strain decreases,the position of crack initiation gradually changes from the surface to the interior.Moreover,the secondary cracks extending inward along the fracture surface appear in the crack initiation area,and there is obvious stress concentration near the secondary cracks.The dislocation density is high near the fracture surface in the crack initiation zone,where a lot of dislocations cutting into the γ'phase exist.An oxide layer of 50‒100 nm is presented on the fracture surface,and Ni,Al,Cr and Co elements are mainly segregated into the oxide layer of the surface.展开更多
文摘Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle.
基金supported by the Tianjin Technical Expert Project (No.19JCTPJC43800)the Tianjin Science and Technology Planning Project (No. 19YFFCYS00090)+1 种基金the Key Project of Science and Technology Cultivation of Tianjin Sino-German University of Applied Technology(No. zdkt2017-006)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The fatigue test between 10^5—10^9 cycles of GH4169 nickel‑based superalloy commonly used in aircraft engines is carried out by ultrasonic fatigue machine at 650℃.The S‑N curve is obtained and the fatigue fracture morphology is observed.The fatigue S‑N curve presents a“step‑like”shape,with the first inflection point near 1×10^7 cycles and the second inflection point near 1×10^8 cycles.There is no engineering fatigue limit,and it still shows a downward trend after 107 or even 10^9 cycles.The crack initiation location is related to its life.Cracks are generated on the surface below 10^7 cycles,while it is inside above 10^7 cycles.The crack initiation source in the ultra‑high cycle fatigue at 650℃ is mainly the local intergranular fracture and casting defect of the matrix.In the phase of crack propagation,the mixed propagation of intergranular and cleavage is the main form.
基金sponsored by AECC Beijing Institute of Aeronautical Materialsfunded by National High-tech R&D Program of China (863 Program) (No. 2015AA034401)。
文摘The effects of inclusions in powder superalloy FGH96 on low-cycle fatigue life were studied, and a low-cycle crack initiation life prediction model based on the theory of damage mechanics was proposed. The damage characterization parameter was proposed after the construction of damage evolution equations. Fatigue tests of the powder superalloy specimens with and without inclusion were conducted at 530 and 600 ℃, and the model verification was carried out for specimens with elliptical, semi-elliptical, polygon and strip-shaped surface/subsurface inclusion. The stress analysis was performed by finite element simulation and the predicted life was calculated. The results showed a satisfying agreement between predicted and experimental life.
基金National Science and Technology Major Project(J2019-VI-0022-0138)。
文摘Low-cycle fatigue crack initiation behavior of nickel-based single crystal superalloy at 530℃ was investigated.Results show that the behavior of crack initiation is closely related to the maximum strain.When the maximum strain is 2.0%,the fatigue crack is originated at the position of persistent slip bands on the surface of specimen,which is located on the{111}slip plane.No defects are observed at the crack initiation position.When the maximum strain is lower than 1.6%,the cracks are initiated at the casting defects on sub-surface or at interior of the specimen.The casting defects are located on the{100}slip plane vertical to the axial force.The crack is initiated along the{100}slip plane and then expanded along different{111}slip planes after a short stage of expansion.As the maximum strain decreases,the position of crack initiation gradually changes from the surface to the interior.Moreover,the secondary cracks extending inward along the fracture surface appear in the crack initiation area,and there is obvious stress concentration near the secondary cracks.The dislocation density is high near the fracture surface in the crack initiation zone,where a lot of dislocations cutting into the γ'phase exist.An oxide layer of 50‒100 nm is presented on the fracture surface,and Ni,Al,Cr and Co elements are mainly segregated into the oxide layer of the surface.
