厚朴酚与和厚朴酚对大鼠回肠短路电流的影响

目的:研究厚朴酚与和厚朴酚对大鼠回肠短路电流的影响。方法:应用体外Ussing chamber系统评价50μmol/L、100μmol/L、200μmol/L厚朴酚或和厚朴酚对回肠短路电流的作用。结果:厚朴酚与和厚朴酚均能明显抑制大鼠回肠的短路电流,且短路电流呈浓度依赖性降低,同时,其对短路电流的抑制效应显著高于钙通道阻滞剂维拉帕米;不同浓度的厚朴酚与和厚朴酚预处理可以显著降低10μmol/l乙酰胆碱激活的短路电流的增加。结论:厚朴酚与和厚朴酚可抑制大鼠回肠的短路电流,其作用机制可能是作为钙离子拮抗剂,通过调控肠道钙离子通道功能来抑制钙离子或其他离子的转运。

Variation, heredity and selection of effective ingredients in Magnolis officinalis of different provenances

Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officinalis were analyzed by the method of HPLC (High Performance Liquid Chromatogram). The results showed that such qualitative traits as the content of magnolol, content of honokiol, total content of key phenols, and the ratio of magnolo to honokiol differ significantly between the provenances. The provenances with a sharpened leaf tip from the western part of Hubei Province has a highest content of phenols, and that with a concave leaf tip from the Lushan Mountain has a lowest content of phenols. All these four qualitative traits were genetically controlled, with a heritability between 0.8342 and 0.9871 in terms of provenance. In addition, both longitudinal and latitudinal geographical variations could be found, with longitudinal variations being dominant. As a result, 3 superior provenances from Wufeng, Enshi and Hefeng of the western part of Hubei as well as 10 high-quality individuals were selected.

Determination of magnolol and honokiol in traditional Chinese medicine Magnolia officinalis and its preparations by liquid-phase microextraction-back extraction combined with high performance liquid chromatography

Liquid-phase microextraction with back extraction （LPME-BE） combined with high performance liquid chromatography （HPLC） was investigated for the extraction and determination of magnolol and honokiol in Magnolia officinalis, a traditional Chinese medicine （TCM）, and its pharmaceutical preparations, Huo Xiang Zheng Qi peroral liquid and Xiang Sha Yang Wei pellet. Organic solvent, donor and acceptor phases, stirring rate and extraction limes were all factors which can influence the efficiency of extraction and were all optimized during the course of this work. Linear calibration curves were obtained in concentration ranges of 1,56-156 μg/mL for magnolol and 1.10-110 μg/mL for honokiol. Detection limits （S/N = 3） were 0.10 and 0.07 μg/mL, respectively. The relative recoveries were both in the range of 98.3% - 105.1% and RSD was lower than 2.5% .

姜厚朴饮片标准汤剂研究

目的制备16批姜厚朴饮片标准汤剂,测定其出膏率、指标成分含量、转移率及指纹图谱等数据,为其配方颗粒的制备和质量评价提供参考。方法依照中药饮片标准汤剂制备要求,制备16批姜厚朴饮片标准汤剂,参照《中国药典》2015年版方法测定厚朴酚与和厚朴酚含量,计算两者总和的转移率,测定汤剂的出膏率及pH值,并绘制HPLC指纹图谱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱:0～15 min,12%～16%乙腈;15～30 min,16%～28%乙腈;30～42 min,28%～74%乙腈;42～55 min,74%～80%乙腈;柱温40℃;体积流量1 mL/min;检测波长294 nm。结果 16批姜厚朴饮片标准汤剂中厚朴酚与和厚朴酚总和的转移率为6.5%～12.0%,出膏率3.41%～7.14%,pH值4.63～5.43,并用中药色谱指纹图谱相似度评价系统(2012A)软件进行指纹图谱分析,找到了7个共有峰,指认4个,分别为木兰苷B(1号峰)、木兰苷A(2号峰)、和厚朴酚(6号峰)、厚朴酚(7号峰)。对16批姜厚朴饮片标准汤剂进行相似度评价,其相似度均大于0.69。同时,建立了姜厚朴饮片标准汤剂指纹图谱。结论所建立的汤剂制备方法稳定可行,分析方法精密度、稳定性和重复性良好,可用于姜厚朴饮片标准汤剂的质量评价。