[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primer...[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content ( PIC value), clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.展开更多
In the research, 20 indica hybrid rice varieties were investigated under high-dense and low-amount, mid-dense and mid-amount and low-dense and high-amount fertilization conditions in order to explore correlation betwe...In the research, 20 indica hybrid rice varieties were investigated under high-dense and low-amount, mid-dense and mid-amount and low-dense and high-amount fertilization conditions in order to explore correlation between LAI in ful heading stage with yield and to analyze yield differences, yield components, material accumulation and transferring, as wel as correlation between key cultivation factors and yield. Final y, approaches and key technology for yield breakthrough were dis-cussed, which provides theoretical and technological references for rice high yield.展开更多
Effects of varieties on the content of tea pigment, L*, a* and b* value of chromatic aberration of black tea infusion was studied. The correlation relationship between content of TF, TR, TB and L*, a*, b* value ...Effects of varieties on the content of tea pigment, L*, a* and b* value of chromatic aberration of black tea infusion was studied. The correlation relationship between content of TF, TR, TB and L*, a*, b* value was extremely significant at P<0.05. Fresh tea leaves with plucking standard of 1 bud and 2 leaves could man-ufacture black tea with higher content of tea pigment, higher a* and b* value but lower L* value, than those of plucking standard of 1 bud and 3 leaves. But the discrepancy of certain parameter of the two plucking standards changed with vari-eties. Varieties’ quality potential could be concealed by improper plucking standards. Six varieties were divided into four groups on the basis of gross tea polyphenols in fresh tea leaves (GTP), consumption of tea polyphenols to gross tea polyphenols ratio (CTP/GTP) and gross tea pigment to consumption of tea polyphenols ratio (GP/CTP). Hybrid performed the very suitability of black tea manufacturing. To en-sure high quality of made black tea, suitable variety should be chosen and reason-able plucking standard also should be taken into consideration.展开更多
[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local v...[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.展开更多
Genetic relationship of 12 ramie varieties with different drought resistance was analysed by using RAPD.Twenty-five 10-mer random primers were used to amplify the total DMA of these varieties.The results showed that t...Genetic relationship of 12 ramie varieties with different drought resistance was analysed by using RAPD.Twenty-five 10-mer random primers were used to amplify the total DMA of these varieties.The results showed that the amplified products of 12 ramie varieties could be obtained with 12 primers.The length of amplified products was 0.6-5.15 kb.Ninety bands were identified in total,of which 11 bands were common in all 12 varieties and 79 bands were polymorphic.The polymorphic bands were 87.78% of the total amplified bands.According to the result of cluster analysis,12 ramie varieties could be divided into two classes and three groups.It was found that high drought resistant varieties were clustered into different groups or subgroups in the same class,which shows near relationship among them.展开更多
In this study it is aimed to investigate live weight and carcass traits of Anatolian Grey and Anatolian Grey x Brown Swiss crossbred in raised Edirne and Kesan. In this study the authors used 51 Anatolian Grey bulls, ...In this study it is aimed to investigate live weight and carcass traits of Anatolian Grey and Anatolian Grey x Brown Swiss crossbred in raised Edirne and Kesan. In this study the authors used 51 Anatolian Grey bulls, 22 Anatolian Grey heifers and 63 Anatolian Grey x Brown Swiss crossbred bulls. 136 animals in 2-3 years old were used present study. The live weights were found 446.375 ± 20.87 and 508.29 ± 11.88 kg for Anatolian Grey bull and Anatolian Grey x Brown Swiss crossbred bull respectively. Carcass percentage were found 60.57%, 46.84%, 56.87% and the correlation coefficient were calculated between live weight and carcass percentage 0.98, 0.80 and 0.94 for Anatolian Grey bulls, Anatolian Grey heifers and Anatolian Grey x Brown Swiss crossbred bulls, respectively.展开更多
The difference of soluble sugar contents and starch synthetic key enzyme activities between high starch cultivar and low starch cultivar were studied in this experiment, of which FuXuan01, GR891, SC124, and SC201 were...The difference of soluble sugar contents and starch synthetic key enzyme activities between high starch cultivar and low starch cultivar were studied in this experiment, of which FuXuan01, GR891, SC124, and SC201 were used as materials. The results showed that the contents of reducing sugar in root tube of low starch cultivars were higher than those of high starch cultivars in all growth periods, the contents of sucrose in root tube of high starch cultivar were higher than those of low starch cultivars in the early growth period, but, lower in the late growth period, and there were almost no difference in the contents of soluble sugar in root tube between high starch cultivar and low starch cultivar in the early growth period, but notable difference in the late growth period and the soluble sugar contents of low starch cultivars were higher than those of high starch cultivars. It also-showed that the activities of ADPGPpase, SSS, and SBE (starch branching enzyme) of high starch cultivars were higher than those of low starch cultivars. It was evident that there were close correlations between the content of sucrose, reducing sugar and soluble sugar in root tube, and the activities of ADPGPpase, SSS, SBE, and the starch accumulation in the root tubers of cassava. It was quite evident in this experiment that the soluble sugar and starch synthetic key enzyme were main factors controlling starch accumulation in root tubers. These results provided important indication for physiological controlling in high starch cultivation and gene engineering breeding of high starch cultivar of cassava.展开更多
In order to study osmotic adjustment and accumulation of anions and cations in rice (Oryza sativa L.) seedlings under NaCl stress, a greenhouse experiment was conducted using two rice cultivars including IR651 (tol...In order to study osmotic adjustment and accumulation of anions and cations in rice (Oryza sativa L.) seedlings under NaCl stress, a greenhouse experiment was conducted using two rice cultivars including IR651 (tolerant) and IR29 (sensitive). Seedlings were grown in Youshida nutrient solution. Salinity treatments were imposed 21 days after sowing with 0 and 100 mM NaCI and seedlings were harvested 0, 72, 120 and 240 hour after salinization. Water (ψw) and osmotic (ψs) potentials, total soluble sugars and inorganic ions (Na+. K+, Cl-, Ca2+ and Mg2+) concentrations and osmotic adjustment were determined in sixth (youngest) leaf. Salinity caused a substantial biomass reduction in rice seedlings, ψs reduction in IR29 was occurred more than IR651. Water potential decreased in both the cultivars under stress conditions, but IR651 was able to maintain higher ψw and kept better growth till the end of the experiment. Osmotic adjustment was observed in IR651 was about 10 times more than in IR29. K+ accumulation decreased in both cultivars under stress condition while Na+ accumulation increased in both the cultivars with higher increase in IR29 seedlings. CI concentration increased in youngest leaf of both IR29 and IR651. Our results showed that tolerant cultivar was able to make osmotic adjustment faster than the sensitive cultivar using high accumulation of solutes especially total soluble sugars.展开更多
The susceptibility of Sogatella furcifera female adults on N22 to malathion increased as feeding time prolonged, while the day change of susceptibility to isoprocarb showed a inverse tendency. The activities of es...The susceptibility of Sogatella furcifera female adults on N22 to malathion increased as feeding time prolonged, while the day change of susceptibility to isoprocarb showed a inverse tendency. The activities of esterase and carboxylesterase were induced significantly after on N22 for one day, and then declined. The non susceptible level of adults to insecticides and enzyme activities seemed to be heightened after feeding on ASD7, a variety resistant to Nilaparvata lugens. The total phenol content in rice leaf sheath was the highest in N22, and could be regarded as one of the factors, which caused chages in susceptibility of S. furcifera to insecticide after feeding different rice varieties. It was recommended that suitable insecticides and planthopper resistant varieties should be coordinately used in practice so that they contribute more effects respectively.展开更多
Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at leas...Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments.展开更多
In this paper, the concept of toric difference varieties is defined and four equivalent descriptions for toric difference varieties are presented in terms of difference rational parametrization,difference coordinate r...In this paper, the concept of toric difference varieties is defined and four equivalent descriptions for toric difference varieties are presented in terms of difference rational parametrization,difference coordinate rings, toric difference ideals, and group actions by difference tori. Connections between toric difference varieties and affine N[x]-semimodules are established by proving the one-to-one correspondence between irreducible invariant difference subvarieties and faces of N[x]-semimodules and the orbit-face correspondence. Finally, an algorithm is given to decide whether a binomial difference ideal represented by a Z[x]-lattice defines a toric difference variety.展开更多
基金Supported by the National Standard Plan(20051079-T-469)~~
文摘[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content ( PIC value), clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.
基金Supported by Rice Industry Technology System(2010030162011BAD16B05-1)~~
文摘In the research, 20 indica hybrid rice varieties were investigated under high-dense and low-amount, mid-dense and mid-amount and low-dense and high-amount fertilization conditions in order to explore correlation between LAI in ful heading stage with yield and to analyze yield differences, yield components, material accumulation and transferring, as wel as correlation between key cultivation factors and yield. Final y, approaches and key technology for yield breakthrough were dis-cussed, which provides theoretical and technological references for rice high yield.
基金Supported by Black Tea Team of Department of National Tea Industry and Technology System(CARS-23)Key Team of Department of Agriculture of Zhejiang Province(2011006)~~
文摘Effects of varieties on the content of tea pigment, L*, a* and b* value of chromatic aberration of black tea infusion was studied. The correlation relationship between content of TF, TR, TB and L*, a*, b* value was extremely significant at P<0.05. Fresh tea leaves with plucking standard of 1 bud and 2 leaves could man-ufacture black tea with higher content of tea pigment, higher a* and b* value but lower L* value, than those of plucking standard of 1 bud and 3 leaves. But the discrepancy of certain parameter of the two plucking standards changed with vari-eties. Varieties’ quality potential could be concealed by improper plucking standards. Six varieties were divided into four groups on the basis of gross tea polyphenols in fresh tea leaves (GTP), consumption of tea polyphenols to gross tea polyphenols ratio (CTP/GTP) and gross tea pigment to consumption of tea polyphenols ratio (GP/CTP). Hybrid performed the very suitability of black tea manufacturing. To en-sure high quality of made black tea, suitable variety should be chosen and reason-able plucking standard also should be taken into consideration.
基金Supported by Program of National Science and Technology Infra-structure (2005DKA21002-09)~~
文摘[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.
文摘Genetic relationship of 12 ramie varieties with different drought resistance was analysed by using RAPD.Twenty-five 10-mer random primers were used to amplify the total DMA of these varieties.The results showed that the amplified products of 12 ramie varieties could be obtained with 12 primers.The length of amplified products was 0.6-5.15 kb.Ninety bands were identified in total,of which 11 bands were common in all 12 varieties and 79 bands were polymorphic.The polymorphic bands were 87.78% of the total amplified bands.According to the result of cluster analysis,12 ramie varieties could be divided into two classes and three groups.It was found that high drought resistant varieties were clustered into different groups or subgroups in the same class,which shows near relationship among them.
文摘In this study it is aimed to investigate live weight and carcass traits of Anatolian Grey and Anatolian Grey x Brown Swiss crossbred in raised Edirne and Kesan. In this study the authors used 51 Anatolian Grey bulls, 22 Anatolian Grey heifers and 63 Anatolian Grey x Brown Swiss crossbred bulls. 136 animals in 2-3 years old were used present study. The live weights were found 446.375 ± 20.87 and 508.29 ± 11.88 kg for Anatolian Grey bull and Anatolian Grey x Brown Swiss crossbred bull respectively. Carcass percentage were found 60.57%, 46.84%, 56.87% and the correlation coefficient were calculated between live weight and carcass percentage 0.98, 0.80 and 0.94 for Anatolian Grey bulls, Anatolian Grey heifers and Anatolian Grey x Brown Swiss crossbred bulls, respectively.
文摘The difference of soluble sugar contents and starch synthetic key enzyme activities between high starch cultivar and low starch cultivar were studied in this experiment, of which FuXuan01, GR891, SC124, and SC201 were used as materials. The results showed that the contents of reducing sugar in root tube of low starch cultivars were higher than those of high starch cultivars in all growth periods, the contents of sucrose in root tube of high starch cultivar were higher than those of low starch cultivars in the early growth period, but, lower in the late growth period, and there were almost no difference in the contents of soluble sugar in root tube between high starch cultivar and low starch cultivar in the early growth period, but notable difference in the late growth period and the soluble sugar contents of low starch cultivars were higher than those of high starch cultivars. It also-showed that the activities of ADPGPpase, SSS, and SBE (starch branching enzyme) of high starch cultivars were higher than those of low starch cultivars. It was evident that there were close correlations between the content of sucrose, reducing sugar and soluble sugar in root tube, and the activities of ADPGPpase, SSS, SBE, and the starch accumulation in the root tubers of cassava. It was quite evident in this experiment that the soluble sugar and starch synthetic key enzyme were main factors controlling starch accumulation in root tubers. These results provided important indication for physiological controlling in high starch cultivation and gene engineering breeding of high starch cultivar of cassava.
文摘In order to study osmotic adjustment and accumulation of anions and cations in rice (Oryza sativa L.) seedlings under NaCl stress, a greenhouse experiment was conducted using two rice cultivars including IR651 (tolerant) and IR29 (sensitive). Seedlings were grown in Youshida nutrient solution. Salinity treatments were imposed 21 days after sowing with 0 and 100 mM NaCI and seedlings were harvested 0, 72, 120 and 240 hour after salinization. Water (ψw) and osmotic (ψs) potentials, total soluble sugars and inorganic ions (Na+. K+, Cl-, Ca2+ and Mg2+) concentrations and osmotic adjustment were determined in sixth (youngest) leaf. Salinity caused a substantial biomass reduction in rice seedlings, ψs reduction in IR29 was occurred more than IR651. Water potential decreased in both the cultivars under stress conditions, but IR651 was able to maintain higher ψw and kept better growth till the end of the experiment. Osmotic adjustment was observed in IR651 was about 10 times more than in IR29. K+ accumulation decreased in both cultivars under stress condition while Na+ accumulation increased in both the cultivars with higher increase in IR29 seedlings. CI concentration increased in youngest leaf of both IR29 and IR651. Our results showed that tolerant cultivar was able to make osmotic adjustment faster than the sensitive cultivar using high accumulation of solutes especially total soluble sugars.
文摘The susceptibility of Sogatella furcifera female adults on N22 to malathion increased as feeding time prolonged, while the day change of susceptibility to isoprocarb showed a inverse tendency. The activities of esterase and carboxylesterase were induced significantly after on N22 for one day, and then declined. The non susceptible level of adults to insecticides and enzyme activities seemed to be heightened after feeding on ASD7, a variety resistant to Nilaparvata lugens. The total phenol content in rice leaf sheath was the highest in N22, and could be regarded as one of the factors, which caused chages in susceptibility of S. furcifera to insecticide after feeding different rice varieties. It was recommended that suitable insecticides and planthopper resistant varieties should be coordinately used in practice so that they contribute more effects respectively.
基金supported by the International Science and Technology Cooperation of China(2011DFA32730)
文摘Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments.
基金supported by the National Natural Science Foundation of China under Grant No.11688101
文摘In this paper, the concept of toric difference varieties is defined and four equivalent descriptions for toric difference varieties are presented in terms of difference rational parametrization,difference coordinate rings, toric difference ideals, and group actions by difference tori. Connections between toric difference varieties and affine N[x]-semimodules are established by proving the one-to-one correspondence between irreducible invariant difference subvarieties and faces of N[x]-semimodules and the orbit-face correspondence. Finally, an algorithm is given to decide whether a binomial difference ideal represented by a Z[x]-lattice defines a toric difference variety.