黄瓜种子乙烯释放量酶活性与品种性型的关系

用节成性不同的黄瓜品种,研究了种子萌发中乙烯释放量,过氧化氨酶(CAT)、过氧化物酶(POD)活性与品种性型表现的关系。结果表明,刚吸胀的种子无乙烯释放,将萌发时乙烯量迅速上升,萌发盛期出现第一个高峰,们根出现时达最高峰,后陡降,CAT和POD活性在种子萌发中波动上升;三者均与雌花节率呈正相关,相关系数分别为0.9925＊＊,0.9768“,0.9481“。乙烯直接影响性型,CAT和PO D通过乙烯影响性型,与乙烯释放量的相关系数依次为0。9964”“和0.9959””·

Comparison of Several Statistical Analysis Models for Genotypic Stability of Saccharum officinarum

[Objective] The study aimed to compare several statistical analysis models for estimating the sugarcane （Saccharum spp.） genotypic stability. [Method] The data of sugarcane regional trials in Guangdong, in 2009 was analyzed by three models respectively： Finlay and Wilkinson model： the additive main effects and multiplicative interaction （AMMI） model and linear regression-principal components analysis （LR- PCA） model, so as to compare the models. [Result] The Finlay and Wilkinson model was easier, but the analysis of the other two models was more comprehensive, and there was a bit difference between the additive main effects and multiplicative inter- action （AMMI） model and linear regression-principal components analysis （LR-PCA） model. [Conclusion] In practice, while the proper statistical method was usually con- sidered according to the different data, it should be also considered that the same data should be analyzed with different statistical methods in order to get a more reasonable result by comparison.

Analysis and Evaluation Indicator Selection of Chilling Tolerance of Different Cotton Genotypes

[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively high photo- synthetic electron transport capacity and high stomatal conductance after recovery treatment, which is contributed to gas exchange and recovery of photosynthetic ca- pacity. Determination of the six indicators under the same stress condition can be adopted for rapid identification and prediction of the chilling tolerance of other cotton varieties, which provides basis for the breeding, promotion, identification and screen- ing of chilling tolerant germplasms.

A New Hot Pepper Cultivar Hangjiao Hongfeng

Hangjiao Hongfeng is a new hot pepper F1 hybrid cultivar which is bred with the cytoplasmic male sterile line 11066 AH as the female parent and the restoringline 11044 CH as the male parent. Its fruit is early-mid maturing and in the shape of a long finger.The immature fruit is in dark-green and mature fruit is in dark-red.The average length, width and flesh thickness are 15.7, 1.9 and 0.17 cm, respectively. The yield of dry pepper is 6 750 kg/hm^2. The average contents of Vc, soluble solid and dry matter are 1.30 mg/g, 10%, and 14% respectively. The cultivar has good resistance to pepper blight, viral disease and anthracnose. It shows strong tolerance to heat, water logging and drought. It is suitable for spring field cultivation in Gansu, Xinjiang and Inner Mongolia.

Genetic analysis of fertility restoring genes for AL-type male sterility in wheat

In order to screen molecular markers linked to fertility restoring genes and further improve the breeding efficiency of restorer lines, in this study, wheat varieties 18A, 18B and 99AR144-1 were used as experimental materials to establish F2 fertility-segregating population. Plant quantitative trait ＂major gene ＋ polygene mixed mo- del＂ separation analysis method and simple sequence repeat （SSR） molecular markers were adopted for genetic analysis of four generations, including the parents （P~ and P2）, and hybrid （G and G） populations. The results show that AL-type fertility restoring gene is controlled by two pairs of additive-dominant-epistatic genes and addi- tive-dominant polygene; two primers linked to fertility restoring genes were selected by SSR molecular markers, including Xgwm95 on chromosome 2A and Barc61 on chromosome 1B, with the linkage distance of 15.0 cM and 18.0 cM, respectively. Based on verification, these two markers are reliable for distinguishing AL-type wheat ste- rile lines and restorer lines.

Studies on Breeding and Application of Thermo-sensitive Genie Male Sterile Line 402S in Brassica napus Ⅰ. Hie Breeding Procedure and Character of 402S

A new rapeseed TGMS line 402S has been bred through six years with seven generations by hybridization and systematic breeding.402S possesses the similar thermo-sensitive male sterility to its female parent Xiangyou 91S which performed fertile,partially sterile and sterile throughout the flowering stage,and resembles its male parent Zhongshuang 4 on agronomic traits.And 402S has the double-low (low erutic acid and low glucosinolate) quality like its original parents.

Genetic Diversity of Italian Ryegrass Diploid Cultivars, Revealed by Electrophoretically Detected Genotypes in Phosphoglucose Isomerase （PGI）

Nine diploid cultivars of Italian ryegrass （Lolium multiflorum Lain.） from France （Fortyl, Vertyl and Jericho）, Germany （Ligrande）, United Kingdom （Aber Epic and Aber Mario）, Denmark （Cordelia）, Netherlands （Alamo） and Poland （Tur） were tested with horizontal gel electrophoresis according to one locus （with four alleles） of the PGI enzyme system. One of them, named P4 is typical for the species, therefore can serve as a good marker for hybrids identification. Each cultivar was characterized by frequencies of different phenotypes. They were highly polymorphic （Pg = 0.58 - 0.78） and showed differences in heterozygosity level. The variability within populations （GST = 0.055） was higher than among populations （DST = 0.032）.

Adaptability Analysis of Hybrid Castor Based on AMMI Model

[Objective] The paper analyzed production adaptability and stability of 4 hybrids of castor,so as to explore the application method of AMMI model in variety evaluation.[Method] AMMI model and biplot were used to carry out stability analysis on 4 castor combinations in multi-point test during 2008 and 2009,and further evaluate the stability and adaptability of various tested combinations.[Result] AMMI model analysis showed that the singular values IPCA1 and IPCA2 in principal component axis of interaction totally explained 92.24% of the interaction variance.AMMI model not only maximizes the reflection on variation of interaction,but also can accurately analyze the adaptability and yield stability of varieties.[Conclusion] AMMI model integrates variance analysis and principal component analysis together,which improves accuracy compared with the traditional method.Even the adaptability of female line without fertility could be indirectly evaluated through the identification on the genotype and environment interaction effects in F1 generation.

Anther Culture of Chinese Radish(Raphanus sativus L. var. Longinnatus Bailey):Response of Different Genotypes to the Embryogenesis and the Traits of Regenerated Plantlets

[Objective] The aims were to ① conduct anther culture of Chinese radish varieties; ② observe the development of embryos from anther culture; ③ study the response of different genotypes to embryogenesis in anther culture; ④ observe the morphology of regenerated plantlets; ⑤ analyze the ploidy level of regenerated plantlets arising from the anther culture process. [Method]Anthers of 15 genotypes with diverse genetic backgrounds of Chinese radish have been cultured in vitro and induced to undergo embryogenesis and plant formation. [Result] Of 15 genotypes evaluated,four produced embryos. The genotype was the main factor to influence the embryogenesis. The morphology and the ploidy of the regenerated plantlets were observed,and the anther-derived plantlets included a mix of haploids,diploids and hexaploids. Of the plants that regenerated from anther embryos 60% were diploid. [Conclusion] The plantlets had the high ability to double spontaneously.

Evaluation Model Construction of Peak Sweet Processing Suitability of Different Varieties of Chestnut

[Objective] The aims was to construct peak sweet processing suitability evaluation model and determine the suitable varieties of peak sweet processing in chestnut. [Method] ＂Zaofeng＂, ＂Yah Long＂ etc. 15 chestnut varieties in Yan Shan area were taken as research objects and investigated the sensory, physicochemical nutrition and processing indexes. The correlation analysis, principal component analysis and cluster analysis were adopted to simplify and calculate the evaluation index, and set up the mathematical model. [Result] Obvious differences in different varieties of raw materials and products of each index and some indicators existed significant correlation relationship; principal component analysis determined the five principal components： hardness, b value, moisture content, total sugar, browning degree and edible rate or the core indicators of quality evaluation in peak sweet chestnut, and the establishment of products comprehensive value scoring model： Y=0.033 509 hardness ＋0.033 509 b value ＋0.f85 1＇73 moisture content ＋0.208 983 total sugar ＋0.108 499 browning degree ＋0.430 327 ratio of feed, peak sweet chestnut quality and raw material associated model： Y=-1.109＋0.015 ratio of good fruit -0.018 kernel hardness ＋0.008 starch, [Conclusion] Peak sweet chestnut processing suitability evaluation model can provide the basis for chestnut processing and the suitable processing of the chestnut breeding; ＂Yanguang＂, ＂Yan Long＂, ＂Yankui＂, are very suitable, and ＂Zibo＂, ＂Yah Ping＂ are more appropriate for peak sweet processing.

Studies on Breeding and Application of Thermo-sensitive Genie Male Sterile Line 402S in Brassica napus Ⅱ.Evaluation of Heterosis and Combining Ability

Augmented randomized complete block test was conducted to evaluate the heterosis of 80 hybrid combinations from TGMS line 402S and its original parent Xiangyou 91S,and the combining a-bility of 40 testcrossing lines.The results of identification test showed that among 47 combinations yielding over the control Xiangyou 15,17 ones with 402S and 3 ones with Xiangyou 91S overyielded more than 20%,reaching the significant level of 1 %;and among 51 combinations yielding over their corresponding higher yield parents,18 ones with 402S and 9 ones with Xiangyou 91S overyielded at 5% or 1% significant level.The test for the GCA effect of all parents indicated that 402S possessed a stronger combining ability than Xiangyou 91S on yield,ailiquae of main inflorescence,total siliquae per plant,seed yield of single plant and 1000 seed weight.10 testcrossing lines with high GCA were picked out for next testcrosses.Among 8 agronomic traits,total siliquae per plant and seed yield of single plant were regarded as the key selecting indexes according to the correlation analysis between yield and the agronomic traits on heterosis and on the GCA effect of all parents.

Vegetative Habitus and Fruit Production of Self-rooted Cherry Cultivar ＇Hedelfingen＇ Wild Type and Somaclonal Grafted on ＇Gisela 6＇ and ＇Colt＇ Rootstock

The study considers the morphological and physiological behaviour of self-rooted sweet cherry CV （Cultivar） ＇Hedelfinger＇ wild type （H） and somaclonal （HS） grafted on ＇Gisela 6＇ and ＇Colt＇ rootstock. The somaclonal showed reduced vegetative vigour without any variation of the natural tree＇s architecture. The rootstock ＇Gisela 6＇ caused change in genotype habitus inducing a spreading shape, while ＇Colt＇ increased trunk diameter and height. Fruit quality and size were not affected by genotype nor rootstock. ＇Gisela 6＇, from these preliminary data, had proved the most suitable rootstock for both genotypes since it reduced the tree size and vigor and induced early bearing and the production of a greater number of fruiting spurs.

Variation in Salt Tolerance of Wheat Cultivars:Role of Glycinebetaine and Ethylene

Four wheat （Triticum aestivum L.） cultivars 711, PBW343, 3765 and WH542 were screened for studying variations in glycinebetaine （GB） content and plant dry mass under 100 mmol L-1 NaCl stress. A tolerance index was calculated using plant dry mass data to select salt-tolerant and salt-sensitive types and find association between tolerance index and GB content. Tolerance index has been used as a good criterion to select the tolerant types under high salinity stress. Further, physiological differences in salt-tolerant cultivar 711 and salt-sensitive cultivar WH542 were examined. The salt-tolerant cultivar exhibited greater CB content, which was found correlative with ethylene. The cultivar also showed higher nitrogen （N） content and nitrate reductase activity, reduced glutathione and higher redox state resulting in maximal protection of plant dry mass than the salt-sensitive type. Thus, the content of CB may be considered as important physiological criteria for selecting salt-tolerant wheat types.