[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primer...[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.展开更多
[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was int...[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.展开更多
This study is a review of existing reports and summary of some field experiences aiming at building of a resilient wheat seed system to resist rust risks in Ethiopia. Practical gaps in the wheat seed system were ident...This study is a review of existing reports and summary of some field experiences aiming at building of a resilient wheat seed system to resist rust risks in Ethiopia. Practical gaps in the wheat seed system were identified with regard to understanding of seed system factors that build, or undermine resilience to rust risks. The study has recognized that the formal seed multiplication schemes are too slow to respond to the ever increasing frequencies of wheat rust outbreaks. Therefore, it is indicated that new approaches such as pre-release and farmer based seed multiplication schemes are helpful in insuring availability of seed of rust resistant wheat varieties to the small farmers.展开更多
基金Supported by the National Standard Plan(20051079-T-469)~~
文摘[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.
基金Supported by Jiangsu High-tech projects(BG2007322) "Good feature of new varieties of Silkworm BreedingPh.D., University of Jiangsu Science and Technology Fund~~
文摘[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.
文摘This study is a review of existing reports and summary of some field experiences aiming at building of a resilient wheat seed system to resist rust risks in Ethiopia. Practical gaps in the wheat seed system were identified with regard to understanding of seed system factors that build, or undermine resilience to rust risks. The study has recognized that the formal seed multiplication schemes are too slow to respond to the ever increasing frequencies of wheat rust outbreaks. Therefore, it is indicated that new approaches such as pre-release and farmer based seed multiplication schemes are helpful in insuring availability of seed of rust resistant wheat varieties to the small farmers.
