骆驼蓬种子凝集素粗品活性的初步研究

采用硫酸铵分级沉淀法提取骆驼蓬种子凝集素粗品,并进行凝血活性和抗菌活性检测。凝血实验结果表明：（1）50%-60%硫酸铵饱和度下提取骆驼蓬种子的凝集素粗品对鸡红细胞的凝集活力最强,最低凝集浓度为0.6 mg/L,且其凝集素粗品的活力受温度、pH值、金属离子及糖溶液的影响。（2）不同硫酸铵饱和度沉淀获得的凝集素粗品对6种人体致病细菌及5种能引起水果腐烂和粮食霉变的真菌均有不同程度的抑制作用,其中50%-60%硫酸铵饱和度下提取的凝集素粗品抑菌活性最强,其浓度为1.071 mg/mL时对鲍曼不动杆菌和意大利青霉菌的抑菌环半径分别可达8.1 mm和4.9 mm。

烯丙孕素对照品的研制及标定

研制了烯丙孕素对照品并对其进行标定。采用紫外光谱、红外光谱、核磁共振波谱、质谱和X射线单晶衍射进行结构确认,高效液相色谱法进行纯度检查,质量平衡法进行定量分析,并用核磁共振波谱法对赋值结果进行验证。结果显示,烯丙孕素对照品原料纯度为99.96%;质量平衡法赋值结果为99.92%,定量核磁共振法验证结果为99.60%。烯丙孕素工作对照品定量赋值结果准确、可靠,能够用于烯丙孕素原料及制剂的鉴别和含量测定。

中药保健再生纤维素纤维的开发及应用

为开发中药保健纤维素纤维,结合中药提取物技术,研究中药保健再生纤维素纤维的制备技术,介绍其工艺流程和生产工艺特点。重点对该类纤维的形态结构、物理机械性能、抗菌性能及纤维性能特点进行分析,同时,对中药保健再生纤维素纤维的开发应用领域进行分析,并提出该类产品制备技术的两大发展趋势,包括微胶囊化技术和吸附材料微粒化技术,为中药保健再生纤维素纤维的开发和生产提供参考。

Novel Substrate pn Junction Isolation for RF Integrated Inductors on Silicon

A new method for reducing the substrate rated losses of integrated spiral inductors is presented.The method is to block the eddy currents induced by spiral inductors by directly forming pn junction isolation in the Si substrate. The substrate pn junction can be realized by using the standard silicon technologies without any additional processing steps.Integrated inductors on silicon are designed and fabricated. S parameters of the inductor based equivalent circuit are investigated and the inductor parameters are calculated.The impacts of the substrate pn junction isolation on the inductor quality factor are studied.The experimental results show that substrate pn junction isolation in certain depth has achieved a significant improvement.At 3GHz,the substrate pn junction isolation increases the inductor quality factor by 40%.

Effect of Main Cultivation Factors on Quality Combination Parameters of High-quality Brassica napus Hybrids

[Objective] This study aimed to analyze the influence of five cultivation factors including sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on the quality parameters of Brassica napus hybrid＆ [Method] Statistical analysis on the data from previous studies and our work was carried out [Result] The contents of erucic acid, thioglycoside, oil, oleic acid and linoleic acid were negatively correlated with the sowing date, while the contents of seed protein, palmitic acid, stearic acid and linolenic acid were positively correlated with the sowing date. The total amount of fat and protein, the ratio of unsaturated fatty acid （UFA） to saturated fatty acid （SFA）, the fatty acid （FA） of erucic acid chain, the total amount of oleic acid and linoleic acid were negatively correlated with the sowing date; the UFA content, the unsatu- ration index, the SFA content, the 18-C FA content, and the ratio of UFA to FA in erucic acid chain were positively correlated with the sowing date. The contents of erucic acid, thioglycoside, seed protein, oleic acid, linolenic acid and eicosenoic acid were negatively correlated with the plant density, while the contents of oil, palmitic acid, stearic acid and linoleic acid were positively correlated with the plant density. The total amount of fat and protein （F ＆ P）, and the ratio of UFA to FA in erucic acid chain were negatively correlated with the plant density, while the UFA content, the unsaturation index, the SFA content, the ratio of UFA to SFA, the 18-C FA content, the FA in erucic acid chain, and the total amount of oleic acid and linoleic acid were positively correlated with the plant density. The contents of erucic acid, thioglycoside, oil, protein in milled rapeseed, palmitic acid and eicosenoic acid were negatively correlated with the nitrogen fertilizer amount, while the contents of seed protein, oleic acid, stearic acid, linoleic acid and linolenic acid were posi- tively correlated with the nitrogen fertilizer amount; the total F ＆ P, the unsaturation index and the ratio of UFA to FA in erucic acid chain were positively correlated with the nitrogen fertilizer amount, while the UFA content, the SFA content, the FA in erucic acid chain, the total amount of oleic acid and linoleic acid, the ratio of UFA to SFA, and the 18-C FA content were negatively correlated with the nitrogen fertilizer amount The contents of erucic acid, thioglycoside, seed protein, linoleic acid and linolenic acid were negatively correlated with the potash fertilizer amount, while contents of oil, oleic acid, stearic acid and eicosenoic acid were positively correlated with the potash fertilizer amount; the total F ＆ P, the unsaturation index, the SFA, and the ratio of UFA to FA in erucic acid chain were negatively correlated with the potash fertilizer amount There were negative correlations between the contents of erucic acid, thioglycoside, seed protein, palmitic acid, stearic acid, linoleic acid, linolenic acid and phosphate fertilizer amount; positive correlations between the contents of oil, oleic acid, eicosenoic acid and phosphate fertilizer amount The total UFA, the unsaturation index, the 1843 FA, the SFA, the FA in erucic acid chain, and the ratio of UFA to FA in erucic acid chain were negatively correlated with the phosphate fertilizer amount [Conclusion] This study identified the correlations of the five cultivation factors with the quality parameters of high-quality Brassica napus hybrids, to provide theoretical basis for its cultivation.

A Novel Lateral Solenoidal On-Chip Integrated Inductor Implemented in Conventional Si Process

A new structure of the on- chip integrated inductors im plem ented in conventional Si process is presented as a lateral solenoid.The fabrication process utilizes a conventional Si technology with standard double- layer m etal- lization.S param eters of the inductors based equivalent circuit are investigated and the inductor parameters are cal- culated from the m easured data.Experimental results are presented on an integrated inductors fabricated in a lateral solenoid type utilizing double m etal layers rather than a single metal layer as used in conventional planar spiral de- vices.Inductors with peak Q of 1.3and inductance value of 2 .2 n H are presented,which are com parable to conven- tional planar spiral inductors.

Effect of Main Cultivation Factors on Oil Quality and Stability of Brassica napus Hybrids

[Objective] This study aimed to analyze the influence of five cultivation factors： sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on oil quality and stability of Brassica napus hybrids. [Method] Statistical analysis was carried out based on the data from previous studies and our work. [Result] Sowing date had no significant influence on oil quality and stability of B. napus hybrids. The plant density shared a positive correlation with 18-C fatty acid （FA） content, unsaturated fatty acid （UFA） content, total content of linoleic acid and linolenic acid, index of unsaturated fatty acid （IUFA）, so a large plant density can improve the oil quality （by increasing18-C FA content, UFA content, total content of linoleic acid and linolenic acid）, but reduce the oil stability （by increasing IUFA）. The amount of nitrogen fertilizer had no significant influence on the contents of 18-C FA and UFA, but significant positive correlations with the total content of linoleic acid and linolenic acid and IUFA. So, the total content of linoleic acid and linolenic acid can be reduced by increasing nitrogen amount, meanwhile the oil stability was also decreased. Phosphate fertilizer amount did not affect 18-C FA significantly, but was negatively correlated with the UFA content, total content of linoleic acid and linolenic acid, and IUFA. So, increasing the amount of applied phosphate fertilizer can reduce UFA content, total content of linoleic acid and linolenic acid, thereby improving oil stability. Potash fertilizer had no significant influence on the IUFA, a significant positive correlation with 18-C FA content, an insignificant positive correlation with the the UFA content, and a negative correlation with the total content of linoleic acid and linolenic acid. The oil content of rapeseed shared an extremely significant positive correlation with 18-C FA content and UFA content, an negative correlation with the total content of linoleic acid and linolenic acid, and no significant correlation with IUFA. [Conclusion] This study identified the effects of five main cultivation factors on oil quality and stability of B. napus hybrids, and the correlations of oil content with 18-C FA content, UFA content, total content of linoleic acid and linolenic acid, and IUFA.

苦豆子凝集素粗品对9种常见致病菌的抑制效果

目的研究苦豆子种子凝集素粗品对几种常见细菌的抑制作用。方法获得不同硫酸铵饱和度下苦豆子种子凝集素粗品,用鸡血和鼠血红细胞分别检测其凝集活性,并以表皮葡萄球菌、粪肠球菌、臭鼻克雷伯菌等9种细菌作为供试菌,比较不同硫酸铵饱和度下凝集素粗品的抑菌活性。结果苦豆子种子凝集素粗品对鸡、鼠血红细胞有不同程度的凝集作用,对9种供试菌均有抑菌活性,其中对福氏志贺氏菌、肺炎克雷伯氏菌、类产碱假单胞菌和大肠埃希菌的抑制效果明显。结论结果表明苦豆子凝集素粗品具有抑制细菌生长的作用,其中对所选革兰氏阴性菌(G-)的抑菌效果好,本研究为苦豆子凝集素将来进一步开发成临床抗菌药物提供了实验依据。

Correlation between Carotenoids Content and Several Quality Traits of Wheat Varieties

A total of 31 wheat varieties（lines） released in different years in Huanghuai Area were selected as materials so as to study the carotenoids content,lipoxygenase activity, and some other quality traits of wholemeal. Results showed that carotenoid content and other quality traits had highly significant differences among varieties. Carotenoids content was significantly positive correlated with watersoluble pentosan and yellowness. Carotenoids content was significantly negative correlated with peak viscosity, hold through, breakdown, final viscosity, unit weight,formation time, and was negative correlated with peak time, protein content and stability time. According to cluster analysis, carotenoid contents in 31 varieties were divided into three major groups： one group had common carotenoid content, which included 19 varieties such as Meng 0318; one group had relatively low carotenoid content, which included 10 varieties such as Luomai23; and the last group had relatively high carotenoid content, which included 2 varieties such as Huaimai22. The average carotenoid contents of the three groups were 1.93, 1.07 and 2.99 mg/kg respectively.

Effect of N Fertilization on Yield, N Absorption and Utilization of Two Species of Super High-Yielding Summer Maize

[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilization in accordance with different cultivars. [Method] Field experiment was conducted to study on effects of different N fertilizers on yield, N absorption and use efficiency of Zhengdan 958 and Xundan 20, in order to learn the effect differences at different N fertilizer levels. [Result] After N was applied, yields of the two summer maize increased significantly. Zhengdan 958 achieved the highest in yield and proceeds at 12 051.18 kg/hm2 and 1 722.40 yuan/hm2, respectively in low N level. In contrast, Xundan 20 achieved the highest at 13 166.00 kg/hm2 and 1 343.92 yuan/hm2 in the above two aspects in high N level. Compared with Zhengdan 958, Xundan 20 increased by 9.90%, 5.20% and 12.00% in N levels of 0, 240, and 450 kg/hm2, respectively. When N fertilizers were applied, protein yield of Xundan 20 was significantly higher than that of Zhengdan 958, so that higher N fertilizers contributed higher protein yield for Xundan 20. In high N level, N efficiency, N-fertilizer utilization and partial productivity of Xundan 20 were significantly higher than that of Zhengdan 958. [Conclusion] Lower N-fertilizer was suitable for Zhengdan 958 and Xundan 20 would get a good harvest if more N-fertilizers were applied. The results provided references for reasonable N fertilization.

Analysis and Optimum Design of Differential Inductors Using Distributed Capacitance Model

A distributed capacitance model for monolithic inductors is developed to predict the equivalently parasitical capacitances of the inductor.The ratio of the self-resonant frequency (f SR) of the differential-driven symmetric inductor to the f SR of the single-ended driven inductor is firstly predicted and explained.Compared with a single-ended configuration,experimental data demonstrate that the differential inductor offers a 127% greater maximum quality factor and a broader range of operating frequencies.Two differential inductors with low parasitical capacitance are developed and validated.

Mineral Elements in Different Varieties of Camellia sinensis

To understand the mineral elements in different varieties of tea （ Camellia sinensis）, 17 mineral elements in eight tea varieties including Yunnan Dayezhong, No. 43 Longjing and No. 6 Zaobaijian, were measured. The results showed that nine elements, such as P, K, Ba, Mn, Cu, were significantly different among varieties, others did not. Black tea varieties usually contain high contents of Cu and K. As a conclusion, mineral elements should be extensively considered in breeding tea varieties.

The Analysis of the Influencing Factors on the Implementing Effects of Plant Breeders' Rights(PBR)System in China

The implementing effects of plant breeders＇ rights（PBR）system is influenced by many factors,such as knowledge of the PBR system,the expense of time for the application and approval,the cost of the applying case,etc..Statistical analysis was carried out based on the above influencing factors by means of questionnaire to find out specific reasons.The result suggests that weak propagandizing;inappropriate propagandizing patterns and obstructed approaches for new regulations are all negatively contributing to the PBR.Meanwhile,current PBR system in China which has been found with many irrational aspects affected the impractical design of regulatory operation and the examining efficiency.Lastly,several suggestions were offered on the base of the results.

Correlation Analysis between Quality Characteristics and Fruit Mineral Element Contents in 'Fuji' Apples

[Objective] The aims were to explore the relationship between the contents of fruit mineral elements and quality features of the ＇Fuji＇ apple, screen major mineral elements of the fruit affecting fruit quality features, and set up optimum proposals of fruit mineral elements for good fruit qualities, so as to provide a theoretical basis for the reasonable orchard soil and foliar fertilizer applications to increase fruit quality and reduce the physiological diseases related to the ＇Fuji＇ apple. [Methods] The fruit mineral elements and quality indicators of ＇Fuji＂ apples were in- vestigated and analyzed, which were collected from the 153 commercial apple or- chards of ＂Fuji＇ apple located in 51 counties. The variable importance for projection （VlP） of partial least squares regression （PLS） method was used to analyze the model effect and weight analysis impact of the fruit mineral element contents to fruit quality, screen out major factors of fruit mineral elements influencing the different fruit qualities, and set up the regression equation of the fruit qualities and major fruit mineral elements. Linear programming was used to obtain optimum proposals of the fruit mineral elements to achieve good ＇Fuji＇ apple qualities. [Results] The mineral elements content and quality characteristics in ＂Fuji＇ apple fruit had great differences in the different produce regions in which the maximum content of nitro- gen, iron, zinc and boron in the ＇Fuji＇ fruit were12.06, 6.17, 7.7, and 10.08 times greater than the minimum respectively, and the differences for titratable acid and the SSC/TA ratio were 2.33 and 2.16 times respectively. The correlation analysis between the fruit mineral element contents and qualities showed that the nitrogen content of fruit had a significantly negative correlation with the soluble solid content, SSC/TA ratio and red color area, while the calcium and iron contents in the fruit were in significantly positive correlation with the soluble solid content and SSC/TA ratio. The model effect and weight analysis showed that the content of nitrogen and iron in the fruit had a greater influence on the integral fruit quality, followed by phosphorus, potassium and calcium. The variable importance for projection （VlP） technology of PLS found that the mean fruit weight was primarily affected by nitro- gen, phosphorus and potassium, and the soluble solid was primarily affected by ni- trogen, calcium and iron, while the red color area was primarily affected by nitro- gen, potassium, calcium, iron and zinc. The regression equation between fruit quality and mineral element contents showed that the mean fruit weight had a greater pos- itive effect coefficient with the content of phosphorus and potassium, and a greater negative effect coefficient with the content of nitrogen in the fruit. Moreover, the sol- uble solid had the largest negative effect coefficient with nitrogen and largest posi- tive effect coefficient with calcium and iron in the fruit. [Conclusion] The maximum content of soluble solid and titratable acid were 1.5 times more than the minimum, and nitrogen, iron, zinc and boron were 6 times more than in the ＇Fuji＇ apple fruit in the different produce regions. Therefore, it is a key technological measure to improve the overall qualities of the ＂Fuji＇ apple by decreasing the content of nitrogen, and increasing the contents of iron, phosphorus, potassium and calcium in the fruit.

Is the Potential Interconnection between Antibiotic Susceptibility and Disinfectant Resistance a Threat to Food Safety?

The prevalence of antibiotic susceptibility and disinfectant resistance in bacterial pathogens causes a serious problem not only to food safety but also to public health, which directly or indirectly leads to treatment and disinfection failures. In this review, multidrug resistance, the mechanism of disinfectant resistance, the methods for detecting disinfectant resistance and the cross-resistance between an- tibiotics and disinfectants are summarized. More efforts should be devoted to explor- ing the professional guidance of using antibiotics and disinfectants, and to develop- ing the comprehensive detection with genetic and molecular methods is highly ex- pected.

Effect of Cultivation Conditionss on Quality Parameters of High Grade Hybrid Rape of Brassica napus L.

[Objective] This study aimed to investigate the combined effects of sowing date, plant density, nitrogenous fertilizer, phosphorus fertilizer and potassium fertilizer on quality parameters of high grade hybrid rapeseed. [Method] By using Youyan 599 and Sanbei 98 as experimental materials, quadratic orthogonal rotation combination with five factors was designed for this study. [Result] Erucic acid content was nega- tively correlated with sowing date and nitrogen fertilizer, positively correlated with plant density. Glucosinolate content was negatively correlated with sowing date, positively correlated with potash fertilizer amount. Oil content was negatively correlated with sowing date and nitrogen fertilizer amount, positively correlated with plant density and potash fertilizer amount. Protein content was positively correlated with sowing date and nitrogen fertilizer amount, negatively correlated with plant density and potash fer- tilizer amount. Oleic acid content was negatively correlated with phosphate fertilizer amount, positively correlated with nitrogen fertilizer amount. Palmitic acid was nega- tively correlated with potash fertilizer amount, positively correlated with sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount in Youyan 599, but negatively correlated with sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount in Sanbei 98. Stearic acid content was posi- tively correlated with sowing date, plant density, nitrogen fertilizer amount and phos- phate fertilizer amount. Linoleic acid was positively correlated with density and nitroge- nous fertilizer, but negatively correlated with potash fertilizer amount. Linolenic acid was positively correlated with nitrogen fertilizer amount and potash fertilizer amount. Eicosenoic acid was negatively correlated with sowing date. There were positive cor- relations and negative correlations among cultivation conditions and quality parame- ters, and the positive correlations and negative correlations could counteract each other. Expression of quality parameters for each treatment was the combined effect. Among the 50 pairs of simple correlations of five cultivation factors and ten quality parameters, 26 pairs were consistent between two varieties, 24 pairs of simple corre- lation were not. Sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount showed significant influence on erucic acid of two varieties, but small effect on palmitic acid. [Conclusion] The combined effect of the five cultivation factors could not fundamentally improve the quality traits of high grade hybrid rapeseed.

Effects of Special Plant Nutritional Elements on Honey Pomelo Fruits

[Objective] The effects of special plant nutritional elements on honey pomelo fruits were investigated. [Method] After the honey pomelo flowers faded, the special plant nutritional elements （SPNE） were sprayed on the honey pomelo plants. After an interval of 20 d or more, the SPNE were sprayed again. A total of 4 times of spraying were carried out before the harvesting. Then difference in yield, quality, fruit weight, fruit appearance, ball weight and internal characteristics were compared between the SPNE treatment group and the control group. [Result] Compared to that of the control group, the fruit number per plant of the SPNE treatment group was more, and the average fruit weight was higher, resulting in the higher yield in the SPNE treatment group. At the same time, the vitamin C and soluble solids con- tent in honey pomelo fruits of the treatment group was higher than that of the con- trol group. The quality of honey pomelo fruits was higher in the SPNE treatment group. The honey pomelo fruits treated with SPNE were bigger, more shining and cleaner. The fruits in the treatment group had no scar, but had more beautiful shape. The exocarp of the treatment group was thinner than that of the control group. The ball weight of the treatment group was higher than that of the control group. Moreover, the ball appearance of the treatment group was cleaner. In the treatment group, the pulp segments were bigger and more consistent in size. In ad- dition, there were no internal cracks in the pulp segments. The fruits of the treat- ment group were more sparkling and more transparent. They tasted better. [Con- clusion] We hoped to provide a theoretical basis for the high-quality and high-yield- ing production of honey pomelo.

Comparions of Required Nitrogen Fertilizers by Hybrid Cultivar and Conventional Cultivar of Rapeseed(Brassica napus L.)

[Objective] The aim of this study was to understand the difference of N fertilizer requirement between hybrid rapeseed and conventional rapeseed. [Method] Two hybrid cultivars, ZY5628 and ZY7819, and the conventional cultivar ZS10, were compared through two field experiments. In Experiment 1, seed yield and optimum N application rate were assessed in the field with five N application treatments. In Expedment 2, N was applied uniformly at 180 kg/hm2, and plant biomass and N accumulation were measured at several developmental stages, while N use efficien- cy was calculated for rape at maturity. [Results] The experiment 1 results showed that seed yields of ZY5628 and ZY7819 were both significantly higher than that of ZS10, and compared to ZS10, optimum yield （plateau yield） was higher by 18.7% and 20.2%, while the recommended N application rate was lower by 9.5% and 9.6% for ZY5628 and ZY7819, respectively. The experiment 2 results showed that during vegetative development, all three cultivars exhibited similar accumulations of plant biomass and N, but through flowering and maturity ZY5628 and ZY7819 pro- duced more biomass, acquired more N, and utilized acquired N more efficiently to- wards seed production than ZS10. [Conclusion] With equivalent inputs, the hybrid rapeseed cultivars ZY5628 and ZY7819 tested herein yield more seed with higher N use efficiency than the conventional rapeseed ZS10. This information will be valu- able for growers seeking to improve efficiency while reducing costs of rape production in China.

Effects of SPNE on Yield and Biochemical Quality of Hybrid Flowering Chinese Cabbage

Objective] The effects of SPNE on the yield and biochemical quality of flowering Chinese cabbage were investigated in this study. [Method] The special plant nutritional element （SPNE） was sprayed on the flowering Chinese cabbage plants after the beginning of harvesting. The effects of SPNE on the yield traits and biochemical quality traits of flowering Chinese cabbage were investigated in the un-conventional using period. [Results] The results showed the flowering stalk of flow-ering Chinese cabbage developed earlier and grew fasterly for the SPNE treatment;the diameter, length and weight of flowering stalk of SPNE-treated flowering Chi-nese cabbage were al higher than that of control. The SPNE treatment could not only increase yield, but also bring better biochemical quality. The protein, vitamin C and calcium contents, especial y the chlorophyl and sugar contents in the SPNE-treated flowering Chinese cabbage were al higher than that of control, making the SPNE-treated flowering Chinese cabbage taste better. [Conclusion] lt was indicated although sprayed after the beginning of harvesting, the SPNE had a certain effect on the flowering Chinese cabbage.