Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production ...Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1 , respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.展开更多
Fly ash has congregated considerable attention as a potential reinforcement for aluminum matrix composites(AMCs)to enhance selective properties and reduce the cost of fabrication.However,poor machinability of such AMC...Fly ash has congregated considerable attention as a potential reinforcement for aluminum matrix composites(AMCs)to enhance selective properties and reduce the cost of fabrication.However,poor machinability of such AMCs limits their application.The present study focuses on the preparation of cenosphere fly ash reinforced Al6061alloys by compo casting method.X-ray diffraction analysis of the prepared AMCs exposes the presence of cenosphere particles without any formation of other intermetallic compounds.In this study,electrical discharge machining(EDM)was engaged to examine the machinability of the prepared metal matrix composite(MMCs).The measured performance characteristics for the various combinations of input process parameters were considered to be MRR,EWR and SR.Face centered central composite design(CCD)of response surface method(RSM)was employed to design the number of experimental trials required and a hybrid approach of grey-based response surface methodology(GRSM)was imposed for predicting the optimal combination of processing parameter in EDM process.Generous improvement was observed in the performance characteristics obtained by employing both the optimal setting of machining parameters.The optical3D surface profile graphs of the ED machined surface also revealed the improvement in surface quality and texture employing the optimal processing conditions proposed by hybrid GRSM approach.展开更多
基金Supported by the National High Technology Research and Development Program of China （2006AA02Z246 2007AA03Z456） the National Natural Science Foundation of China （20776119 21076169）+4 种基金 Xi’an Research and Development Program（CX0735） the Scientific Research Program of Shaanxi Provincial Department of Education China （07JK417 07JC16 JG08181） the Natural Science Foundation of Shaanxi Province （2010JQ2012） the Specialized Research Fund for the Doctoral Program of Higher Education of China （20096101120023 20096101110014） Shaanxi Key Subject Program China
文摘Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1 , respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.
文摘Fly ash has congregated considerable attention as a potential reinforcement for aluminum matrix composites(AMCs)to enhance selective properties and reduce the cost of fabrication.However,poor machinability of such AMCs limits their application.The present study focuses on the preparation of cenosphere fly ash reinforced Al6061alloys by compo casting method.X-ray diffraction analysis of the prepared AMCs exposes the presence of cenosphere particles without any formation of other intermetallic compounds.In this study,electrical discharge machining(EDM)was engaged to examine the machinability of the prepared metal matrix composite(MMCs).The measured performance characteristics for the various combinations of input process parameters were considered to be MRR,EWR and SR.Face centered central composite design(CCD)of response surface method(RSM)was employed to design the number of experimental trials required and a hybrid approach of grey-based response surface methodology(GRSM)was imposed for predicting the optimal combination of processing parameter in EDM process.Generous improvement was observed in the performance characteristics obtained by employing both the optimal setting of machining parameters.The optical3D surface profile graphs of the ED machined surface also revealed the improvement in surface quality and texture employing the optimal processing conditions proposed by hybrid GRSM approach.
