RAPD标记对喀斯特高海拔山区适宜玉米自交系遗传多样性的研究

利用RAPD分子标记技术研究了我国喀斯特高海拔山区适宜玉米自交系的遗传多样性,用23条引物对我国喀斯特高海拔山区适宜玉米自交系进行遗传多样性研究和杂种优势群划分。从90个随机引物中筛选出23个多态性好的引物扩增材料,产生出201条多态带,多态性位点比率为81.6%,通过UPGMA聚类分析,将我国喀斯特高海拔山区玉米地方自交系划分为7个类群。

喀斯特高海拔山区玉米骨干自交系遗传多样性RAPD标记分析

以代表我国玉米6个主要杂种优势群旅大红骨、Lancaster、Reid、苏湾、墨白和塘四平头的标准测验种丹340、自330、792 2、苏37、4 4 9、黄早四和适应我国喀斯特高海拔山区的玉米骨干自交系为材料,利用RAPD标记对其进行遗传多样性和杂种优势群划分。从90个随机引物中筛选出2 1个多态性好的引物扩增材料,共产生14 6条谱带,其中12 4条谱带有多态性,占86 3% ,说明喀斯特高海拔山区的玉米骨干自交系具有较丰富的遗传多样性。通过UPGMA聚类分析,以遗传相似系数为0 6 32 ,可将我国喀斯特高海拔山区玉米种质资源的16个骨干自交系划分为5个类群。

贵州喀斯特高原区圆叶乌桕种群特征研究

以岩石裸露率在80%以上的贵州喀斯特高原区自然生长的圆叶乌桕作为研究对象,分析其种群的年龄结构和种群中的物种组成动态。结果表明:生态环境严酷、资源贫乏的喀斯特高原区,圆叶乌桕生长较好。但是种群内部的圆叶乌桕幼树较少,从森林演替的规律,圆叶乌桕最终被其它物种所代替。从喀斯特区生态环境治理和当地人民生活水平的角度考虑,圆叶乌桕是较为理想的先锋树种之一。

SSR标记对高海拔玉米自交系遗传多样性的研究

利用SSR分子标记技术研究了我国喀斯特高海拔山区主要玉米自交系的遗传多样性，初步进行了杂种优势群划分。37对引物在供试材料中共检测出128个等位基因变异，每对引物检测等位基因2-6个，平均为3．48个，平均多态性信息量为0．506，33个自交系之间的遗传相似系数变化范围为0．476-0．876，平均为0．607。UPGMA聚类分析结果表明，可将我国喀斯特高海拔山区玉米地方自交系划分为6个类群。

Study on Influences of Different Land Use Types on Soil Qualities in Plateau Karst Depression

[Objective] The aim was to study influences of different land use ways on soil qualities in plateau karst depression.[Method] Different land use types were divided by topographic map of 1∶10 000 scales and soil properties of physics and chemistry were analyzed.[Result] The results showed that the variation trend of soil depth(A+B stratum)was slope plough(include returning farm to forest)land>natural forest land>planted forest land>shrub forest land,and relative indexes changing regularities of soil...

Cultivation Techniques for High-yield Corn in a Karst Area of Southwest Guizhou

The cultivation techniques for high-yield corn in a karst area of southwest Guizhou were elaborated in this research from the respects of choosing farmland, preparing farmland, picking good seeds, timely sowing, rational close planting, im-proving the quality of planting, mulching, scientific fertilization, field management, timely harvest, etc. we hoped to provide a reference for the realization of high-effi-ciency corn planting in mountain areas.

Rocky Desertification Risk Zone Delineation in Karst Plateau Area:A Case Study in Puding County,Guizhou Province

Karst rocky desertification is a geo-ecological problem in Southwest China. The rocky desertification risk zone delineation could be used as a guide for the regional and hierarchical rocky desertification management and prevention. We chose the middle and lower reaches of the Houzhai underground basin on the karst plateau in Puding County, Guizhou Province, China as the study area and selected land use type, elevation, slope, aspect, lithology and settlement buffer as the main driving factors of the rocky desertification. The potential risk of rocky desertification was quantifed with the factor-weights union method and statistical analysis method. Five grades of rocky desertification risk were delineated based on Geographic Information System. The extremely low, low, moderate, high and extremely high rocky desertification risk zones accounted for 5.01%, 44.17%, 33.92%, 15.59% and 1.30%, respectively. As a whole, the rocky desertification risk level was moderate because the area of low and moderate rocky desertification risk zones occupied 78.09% of the study area. However, more than half of the area （about 50.81%） was predicted to have moderate rocky desertification risk and above, indicating that the study area was subject to rocky desertification. Rocky desertification risk was higher in the southeast and lower in the northwest of the study area. Distinct differences in the distribution of rocky desertification risk zones corresponding to different factors have been found.

Land cover change in different altitudes of Guizhou-Guangxi karst mountain area, China： patterns and drivers

Topography, especially altitude, will influence the way, process and characteristics of land cover changes in mountainous area, simultaneously, the vertical difference of land cover changes will affect soil quality and regional ecological environment. Therefore, the gradient relationship analysis between land cover changes and altitude is very important for regional sustainability. This study investigated land cover dynamics based on land cover data from a typical mountainous area in the Guizhou-Guangxi karst mountain area, China, in 2000 and 2010, then explored the relationship between altitude and land cover change and analyzed different drivers of land cover change at different altitudes. Our findings are as follows. 1) From 2000 to 2010, the total area of land cover transition was 7167.04 km^2 or 2.8% of the region. The increasing area of build-up land(926.23 km^2) was larger than that of forest(859.38 km^2), suggesting that the urban construction speed was higher than that of reforestation. 2) Intensity of land cover transition in northwestern Guizhou-Guangxi karst mountain area was much larger than that of southeast part and their transition trend was also significantly different, which was consistent with regional population and economy. 3) Human activity was the most dramatic at altitudes between 0–500 m. For 500–1000 m, grassland mainly converted to forest and build-up land. Area of land cover transition was the greatest between 1000–1500 m, while above 1500 m, the transition of grassland was the most obvious. 4) The drivers of land cover change varied. Land cover change was positively correlated with gross domestic product and population density but was inversely related to relief amplitude. There were correlations between land cover change and distance to roads and rivers, and their correlations varied with altitude. By revealing patterns and causes of land cover changes in different altitudes, we hope to understand the vertical dependence of land cover changes, so as to improve land productivity and protect land ecological environment scientifically.

Thermokarst lake changes between 1969 and 2010 in the Beilu River Basin, Qinghai–Tibet Plateau, China

This work presents changes of thermokarst lakes from Beilu River Basin on the Qinghai-Tibet Plateau over the past four decades （1969-2010） using aerial and satellite image interpretation. The results indicated that thermokarst lake activity had generally increased rapidly between 1969 and 2010. The number of thermokarst lakes had increased by approximately 534, and their coverage expanded by about 410 ha. The two main changes observed were an increase in the number of small lakes and the expansion of larger lakes. These changes are likely the result of persistent climate warming and a gradually increasing imbalance between precipitation and evapotranspiration （PET）. However, some non-climatic factors, such as the lake-bottom substrate and local engineering activities, have also influenced the lake changes. If air temperature and P-ET continue to rise, the number of thermokarst lakes and the area they cover may continue to increase in the future.

Application of a ^(137)Cs fingerprinting technique for interpreting responses of sediment deposition of a karst depression to deforestation in the Guizhou Plateau,China

Deforestation to reclaim land often triggers severe soil erosion in the Guizhou Karst Plateau. 137Cs dating of the deposited sediments in the karst depression bottom was used to estimate soil losses by surface erosion since deforestation started in 1979 on hillslopes in the Shirenzhai catchment, Puding County, Guizhou Province. The catchment has a drainage area of 0.054 km2. The average 137Cs contents of the top and peak layers in five cores of the depression bottom (with an area of 2652 m2), collected in 2009, were 2.35 and 7.25 Bq kg-1, respectively. The medium depths in the depression (which ranged between 84 cm and 113.5 cm with a mean value of 92.1 cm) showed the presence of sediments deposited in 1979. The total volume and weight of the deposited sediments since 1979 were estimated to be 1965 m3 and 2496 t, respectively. The depression bottom can be treated as a temporary impoundment and its sediment trapping efficiency was estimated to be 0.7. The relevant average soil erosion rate on the hillslopes was 2315 t km-2 yr-1 since 1979. The mean 137Cs inventory of the five cores was 7693 Bq m-2, which was ~10 times the local reference inventory of 782 Bq m-2. The total 137Cs activity of the sediments in the bottom was 20.4×106 Bq, and the relevant 137Cs inventory loss from the hillslopes was 358 Bq m-2 (since 1954), which accounted for 45.8% of the reference inventory. As soil erosion was not severe before and after the period of deforestation and following cultivation in 1979-1990, the erosion rates on the hillslopes could potentially reach 7000 t km-2 yr-1.

Effect of a thermokarst lake on soil physical properties and infiltration processes in the permafrost region of the Qinghai-Tibet Plateau, China

Changes in the hydrological processes in alpine soil constitute one of the several key problems encountered with studying watershed hydrology and ecosystem stability against the background of global warming. A typically developing thermokarst lake was chosen as a subject for a study using model simulation based on observations of soil physical properties, infiltration processes, and soil moisture. The results showed that the selected thermokarst lake imposed certain changes on the soil infiltration processes and, with the degree of impact intensifying, the initial infiltration rate decreased. The greatest reduction was achieved in the area of moderate impact. However, the stable infiltration rate and cumulative infiltration gradually increased in the surface layer at a depth of 10 and 20 cm, both decreasing initially and then increasing, which is correlated significantly with soil textures. Moreover, the cumulative infiltration changed in line with steady infiltration rate. Based on a comparative analysis, the Horton model helps better understand the effect on the soil infiltration processes of the cold alpine meadow close to the chosen thermokarst lake. In conclusion, the formation of the thermokarst lake reduced the water holding capacity of the alpine meadow soil and caused the hydraulic conductivity to increase, resulting in the reduction of runoff capacity in the area of the thermokarst lake.