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组织培养条件下喜树叶片细胞壁酸性降解的pH值观察(英文) 被引量:3
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作者 祖元刚 于景华 +3 位作者 唐中华 郭晓瑞 孟庆焕 张宇亮 《植物研究》 CAS CSCD 北大核心 2005年第3期286-288,共3页
喜树叶片外植体经组织培养第13d和23d后进行解剖学观察,同时比较正常叶片的解剖学特征,发现在pH值为5.8的酸性培养基中,喜树叶片外植体中的海绵组织和栅栏组织等薄壁细胞相继发生明显的细胞壁降解,而表皮细胞发生较微弱的细胞壁降解现象... 喜树叶片外植体经组织培养第13d和23d后进行解剖学观察,同时比较正常叶片的解剖学特征,发现在pH值为5.8的酸性培养基中,喜树叶片外植体中的海绵组织和栅栏组织等薄壁细胞相继发生明显的细胞壁降解,而表皮细胞发生较微弱的细胞壁降解现象;进行组织培养前,正常叶片的各类细胞未观察到细胞壁降解现象发生。应用BCECFAMpH荧光探标记并采用激光共聚焦在480nm波长下进行pH值测定发现,喜树叶片外植体经组织培养第13d和第23d后的海绵组织和栅栏组织等薄壁细胞部位的pH值均为5.2,但表皮细胞部位的pH值则为5.7~5.8,而正常叶片各类细胞的pH值平均为5.7。这说明,pH值为5.8的酸性培养基和喜树叶片薄壁细胞内的酸性成分自泌可能共同诱导了其细胞壁的酸性降解。 展开更多
关键词 组织培养 喜树叶片 细胞壁酸性降解 PH值 组织培养条件 细胞壁降解 外植体 PH值测定 酸性成分
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喜树叶片次生代谢产物的高效液相图谱分析
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作者 王博文 王萍 +2 位作者 潘华 王洋 阎秀峰 《东北林业大学学报》 CAS CSCD 北大核心 2008年第3期49-50,共2页
借鉴中药指纹图谱的思想,建立了一种快速、全面分析喜树(Camptotheca acuminata)叶片次生代谢产物的高效液相图谱分析方法:C18色谱柱(250mm×4.6mm,5μm),以甲醇-2%(体积分数)乙彰水(mL/mL)为流动相进行梯度洗脱(85... 借鉴中药指纹图谱的思想,建立了一种快速、全面分析喜树(Camptotheca acuminata)叶片次生代谢产物的高效液相图谱分析方法:C18色谱柱(250mm×4.6mm,5μm),以甲醇-2%(体积分数)乙彰水(mL/mL)为流动相进行梯度洗脱(85min),检测波长210~400nm,样品用甲醇超声提取。运用该方法从喜树叶片中检测出11种次生代谢产物。 展开更多
关键词 喜树叶片 高效液相图谱 次生代谢产物
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Nitrate reductase activity and its diurnal variation rhythm for Camptotheca acuminata seedlings 被引量:1
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作者 孙世芹 阎秀峰 《Journal of Forestry Research》 SCIE CAS CSCD 2004年第3期167-170,共4页
Nitrate reductase activity (NRA) in different plant organs and leaves in different positions of Camptotheca acuminata seedlings was determined by an In vivo assay, the diurnal variation rhythm of NRA in leaves of diff... Nitrate reductase activity (NRA) in different plant organs and leaves in different positions of Camptotheca acuminata seedlings was determined by an In vivo assay, the diurnal variation rhythm of NRA in leaves of different positions was observed,and the correlations between leaf NRA, leaf area and lamina mass per unit area (LMA) were also examined. The results showed that NRA in the leaf was significantly highest, compared with that in other organs such as roots, stems and leaves. In this experiment, the 10 leaves were selected from the apex to the base of the seedlings in order. The different NRA occurred obviously in leaves of different positions of C. acuminata seedlings from the apex to the base, and NRA was higher in the 4th-6th leaves.The diurnal change rhythm of leaf NRA showed a one peak curve, and maximum NRA value appeared at about midday (at 12:30 or so). No obvious correlations between NRA and leaf area or lamina mass per unit area were observed. This study offered scientific foundation for the further research on nitrogen metabolism of C. acuminata. 展开更多
关键词 Camptotheca acuminata Nitrate reductase activity Diurnal variation
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