The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column w...The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.展开更多
Yarsagumba(Ophiocordyceps sinensis), an endemic species to the Himalayas and Tibetan Plateau, is one of the most valuable medicinal mushrooms in the world. In Nepal, it is distributed largely in isolated patches of al...Yarsagumba(Ophiocordyceps sinensis), an endemic species to the Himalayas and Tibetan Plateau, is one of the most valuable medicinal mushrooms in the world. In Nepal, it is distributed largely in isolated patches of alpine grasslands of 3,000-5,000 m elevation. Although it is reported from 27 northernmost districts of Nepal, the local distribution pattern of this species is largely unknown.Furthermore, the collection system and local management regime of this species are not well documented. We conducted a field survey at Dhorpatan Hunting Reserve(DHR) among the different stakeholders in January-June 2012 to assess collection sites, patterns and trends and to understand the management regime. We estimated that about 75 kg of Yarsagumba is collected every year from DHR and the amount has been declining since 2008. To manage the resource, locals have initiated regulating the collection by issuing permits, taxing to the collectors, and monitoring the activities of harvesters with the help of park authorities. The revenue generated at local level from the permits has been used for community developmental activities.展开更多
Subalpine forests are known as outstanding habitats due to co-existence of both temperate and alpine vegetation and are classic example of ecotonal zones. Limited but diverse physiognomy of trees inhabiting in subalpi...Subalpine forests are known as outstanding habitats due to co-existence of both temperate and alpine vegetation and are classic example of ecotonal zones. Limited but diverse physiognomy of trees inhabiting in subalpine forest results in variability within under-canopy habitat conditions. Studies were undertaken to assess population status, habitat preference and interferences to the trees and associated under-canopy herbs in a subalpine forest of western Himalaya. A total of lo woody and 23 under-canopy herbs were recorded in the selected subalpine forest area. At each stand, the number of tree species and under-canopy herbs ranged from 2 to 4 and 8 to lo respectively. Abies spectabilis, Acer caesium, Quercus floribunda, Q. semecarpifolia and Rhododendron arboreum were key tree species in this area. The density of main woody species was 280 to 119o individuals ha-1 at different stands. Herbaceous plants with rosette and clump growth habits were observed to have higher values for total basal cover and importance value index. Presence of some under- canopy herbs like; Dactylorhiza hatagirea, Malaxis muscifera, Picrorhiza kurrooa, Polygonatum cirrhifolium habitats also and Skimmia laureola showed that they are in the habitat specific specific. However, the presence of Frageria nubicola and Viola sp. was common in the selected stands. Felling of trees for timber, construction of temporary huts, fuel wood and lopping for fodder were main interferences for trees. On the other hand, trampling driven damage due to grazing, habitats degradation and overexploitation were observed key threats for under-canopy herbs. Integrated analysis including population studies, habitats preference and interferences to the trees and under-canopy herbs in this sensitive and important ecosystem will be useful for determining the conservation plans and ecosystem management.展开更多
Lattice constants, total energies and densities of states of transition metals Fe, Ru and Os with BCC, FCC and HCP structures were calculated by the GGA+PBE functional and the ultrasoft pseudo-potential plane wave met...Lattice constants, total energies and densities of states of transition metals Fe, Ru and Os with BCC, FCC and HCP structures were calculated by the GGA+PBE functional and the ultrasoft pseudo-potential plane wave method, and compared with those of the first-principles projector augmented wave (PAW) method, CALPHAD method and experimental data. The results show that the lattice stability of this work is △GBCC-HCP>△GFCC-HCP>0, agreeing well with those of PAW method in the first-principles and CALPHAD method except for BCC-Fe. And the densities of state of HCP-Ru and Os have an obvious character of stable phase, agreeing completely with the results of the total energy calculations. Further analyses of atomic population show that the transition rate of electrons from s to p state for HCP, FCC and BCC crystals increases from Fe to Os, and a stronger cohesion, a higher cohesive energy or a more stable lattice between atoms of heavier metals are formed.展开更多
基金This paper was supported by National Natural Science Foundation of China (No. 30070086).
文摘The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.
基金the Dhorpatan Hunting Reserve Office and Department of National Parks and Wildlife Conservation, Nepal for the financial and logistic support to conduct this study
文摘Yarsagumba(Ophiocordyceps sinensis), an endemic species to the Himalayas and Tibetan Plateau, is one of the most valuable medicinal mushrooms in the world. In Nepal, it is distributed largely in isolated patches of alpine grasslands of 3,000-5,000 m elevation. Although it is reported from 27 northernmost districts of Nepal, the local distribution pattern of this species is largely unknown.Furthermore, the collection system and local management regime of this species are not well documented. We conducted a field survey at Dhorpatan Hunting Reserve(DHR) among the different stakeholders in January-June 2012 to assess collection sites, patterns and trends and to understand the management regime. We estimated that about 75 kg of Yarsagumba is collected every year from DHR and the amount has been declining since 2008. To manage the resource, locals have initiated regulating the collection by issuing permits, taxing to the collectors, and monitoring the activities of harvesters with the help of park authorities. The revenue generated at local level from the permits has been used for community developmental activities.
文摘Subalpine forests are known as outstanding habitats due to co-existence of both temperate and alpine vegetation and are classic example of ecotonal zones. Limited but diverse physiognomy of trees inhabiting in subalpine forest results in variability within under-canopy habitat conditions. Studies were undertaken to assess population status, habitat preference and interferences to the trees and associated under-canopy herbs in a subalpine forest of western Himalaya. A total of lo woody and 23 under-canopy herbs were recorded in the selected subalpine forest area. At each stand, the number of tree species and under-canopy herbs ranged from 2 to 4 and 8 to lo respectively. Abies spectabilis, Acer caesium, Quercus floribunda, Q. semecarpifolia and Rhododendron arboreum were key tree species in this area. The density of main woody species was 280 to 119o individuals ha-1 at different stands. Herbaceous plants with rosette and clump growth habits were observed to have higher values for total basal cover and importance value index. Presence of some under- canopy herbs like; Dactylorhiza hatagirea, Malaxis muscifera, Picrorhiza kurrooa, Polygonatum cirrhifolium habitats also and Skimmia laureola showed that they are in the habitat specific specific. However, the presence of Frageria nubicola and Viola sp. was common in the selected stands. Felling of trees for timber, construction of temporary huts, fuel wood and lopping for fodder were main interferences for trees. On the other hand, trampling driven damage due to grazing, habitats degradation and overexploitation were observed key threats for under-canopy herbs. Integrated analysis including population studies, habitats preference and interferences to the trees and under-canopy herbs in this sensitive and important ecosystem will be useful for determining the conservation plans and ecosystem management.
基金Project(20070533118) supported by the Doctoral Discipline Foundation of Ministry of Education of ChinaProjects(50471058, 50271085) supported by the National Natural Science Foundation of ChinaProject supported by the Postdoctoral Foundation of Central South University, China
文摘Lattice constants, total energies and densities of states of transition metals Fe, Ru and Os with BCC, FCC and HCP structures were calculated by the GGA+PBE functional and the ultrasoft pseudo-potential plane wave method, and compared with those of the first-principles projector augmented wave (PAW) method, CALPHAD method and experimental data. The results show that the lattice stability of this work is △GBCC-HCP>△GFCC-HCP>0, agreeing well with those of PAW method in the first-principles and CALPHAD method except for BCC-Fe. And the densities of state of HCP-Ru and Os have an obvious character of stable phase, agreeing completely with the results of the total energy calculations. Further analyses of atomic population show that the transition rate of electrons from s to p state for HCP, FCC and BCC crystals increases from Fe to Os, and a stronger cohesion, a higher cohesive energy or a more stable lattice between atoms of heavier metals are formed.
