We evaluated the pathogenicity of six strains 1. fumosorosea to determine their potential as biological control agents of A. ludens by performing bioassays under laboratory conditions （25 ± 2 ℃, 60± 5% R.H...We evaluated the pathogenicity of six strains 1. fumosorosea to determine their potential as biological control agents of A. ludens by performing bioassays under laboratory conditions （25 ± 2 ℃, 60± 5% R.H and 12：12 h L： D） exposing larvae and pupae to a concentration of 1 × 10^8 conidia per milliliter using three different methods： direct spraying, spray of soil and submerged for both instars. The results showed that direct spraying method Pfr-612 strain showed the highest mortality （47%） in pupae while for larvae was strain HIB-27 to 46%. Regarding spray of soil Pfr-612 strain showed the highest percentage of mortality for pupae and larvae with 45 and 57% mortality, respectively. Finally, for the submerged method in pupae HIB-27 strain showed 62% mortality whereas in larvae HIB-32 strain showed 46%. These results indicate that A. ludens is susceptible in at least two instars to L fumosorosea and make this fungus a promising agent for control of the Mexican fruit fly.展开更多
文摘We evaluated the pathogenicity of six strains 1. fumosorosea to determine their potential as biological control agents of A. ludens by performing bioassays under laboratory conditions （25 ± 2 ℃, 60± 5% R.H and 12：12 h L： D） exposing larvae and pupae to a concentration of 1 × 10^8 conidia per milliliter using three different methods： direct spraying, spray of soil and submerged for both instars. The results showed that direct spraying method Pfr-612 strain showed the highest mortality （47%） in pupae while for larvae was strain HIB-27 to 46%. Regarding spray of soil Pfr-612 strain showed the highest percentage of mortality for pupae and larvae with 45 and 57% mortality, respectively. Finally, for the submerged method in pupae HIB-27 strain showed 62% mortality whereas in larvae HIB-32 strain showed 46%. These results indicate that A. ludens is susceptible in at least two instars to L fumosorosea and make this fungus a promising agent for control of the Mexican fruit fly.
