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摄食不同喹乙醇水平饲料的鲤鱼肠道菌群的变化 被引量:2
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作者 卢彤岩 叶继丹 +2 位作者 杨雨辉 刘洪柏 赵吉伟 《水产学杂志》 CAS 2003年第1期17-22,共6页
本文研究了饲料中不同水平喹乙醇对鲤鱼肠道菌群数量及组成的影响。设计 6组不同喹乙醇含量的饲料 (0、 2 0 0、 40 0、80 0、 1 60 0和 32 0 0mg/kg) ,对鲤鱼进行 84d的饲养试验。试验结束时 ,各组随机取 2尾鱼 ,分别对其前、中、后肠... 本文研究了饲料中不同水平喹乙醇对鲤鱼肠道菌群数量及组成的影响。设计 6组不同喹乙醇含量的饲料 (0、 2 0 0、 40 0、80 0、 1 60 0和 32 0 0mg/kg) ,对鲤鱼进行 84d的饲养试验。试验结束时 ,各组随机取 2尾鱼 ,分别对其前、中、后肠的细菌数量和组成进行了分析。结果发现 :与对照组相比 ,投喂喹乙醇的试验组鱼肠道菌群数均有不同程度的下降 ,其中 1 60 0、32 0 0mg/kg组的下降最为明显。从 1 60 0mg/kg开始前肠菌群数明显下降 ,在 40 0mg/kg时中肠菌群开始下降较为明显 ,后肠菌群数在各试验组均呈下降趋势。在对照组检出的 7种主要菌群也是构成各个实验组鱼肠道中的主要菌群 ,但这 7种菌群在各组中的组成比例不同 ,其中鲤鱼肠道菌属中Aer属、Aci属的组成比例变化较大 ,80 0mg/kg (1 1 0 6 % )、 1 60 0mg/kg (1 1 735% )和 32 0 0mg/kg (7 40 % )组中Aer所占比例明显低于对照组 (2 6 2 55 % ) ,而 80 0mg/kg (37 50 2 % )、 1 60 0mg/kg (36 52 % )和32 0 0mg/kg (41 357% )组中Aci所占比例明显高于对照组 (1 3 2 1 3% )。饲料中添加不同水平的喹乙醇可明显影响鲤鱼肠道主要菌群的数量及组成比例。 展开更多
关键词 摄食水平 喹乙醇饲料 鲤鱼 肠道菌群 菌群变化 饲养试验
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Effect of Dietary Olaquindox on the Growth of Large Yellow Croaker(Pseudosciaena crocea R.) and the Distribution of Its Residues in Fish Tissues 被引量:3
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作者 LI Huitao WANG Weifang +3 位作者 MAI Kangsen AI Qinghui ZHANG Chunxiao ZHANG Lu 《Journal of Ocean University of China》 SCIE CAS 2014年第5期820-824,共5页
Olaquindox(OLA), one of quinoxaline-N, N-dioxides, has been put under ban. However it was used as a medicinal feed additive early; it promotes the growth of livestock and prevents them from dysentery and bacterial ent... Olaquindox(OLA), one of quinoxaline-N, N-dioxides, has been put under ban. However it was used as a medicinal feed additive early; it promotes the growth of livestock and prevents them from dysentery and bacterial enteritis. In this study, we evaluated the effect of dietary OLA on the growth of large yellow croaker(Pseudosciaena crocea R.) and the histological distribution of OLA and its metabolite 3-methyl-quinoxaline-2-carboxylic acid(MQCA) in fish tissues. Four diets containing 0(control), 42.5, 89.5 and 277.2 mg kg-1 OLA, respectively, were formulated and tested, 3 cages(1.0 m × 1.0 m × 1.5 m) each diet and 100 juveniles(9.75 ± 0.35 g) each cage. The fish were fed to satiation twice a day at 05:00 am and 17:00 pm for 8 weeks. The survival rate of fish fed the diet containing 42.5 and 89.5 mg kg-1 OLA was significantly higher than that of fish fed the diet containing 0 and 277.2 mg kg-1 OLA(P < 0.05), while the weight gain rate of fish fed the diet containing 42.5 and 89.5 mg kg-1 OLA was significantly higher than that of fish fed the diet without OLA(control)(P<0.05), but similar to that of fish fed the diet with 277.2 mg kg-1 OLA. Fish fed the diet with 277.2 mg kg-1 OLA had the highest content of OLA and MQCA in liver(3.44 and 0.39 mg kg-1, respectively), skin(0.46 and 0.09 mg kg-1, respectively) and muscle(0.24 and 0.06 mg kg-1, respectively). In average, fish fed the diet containing OLA had the highest content of OLA and MQCA in liver which was followed by skin and muscle(P < 0.05), whereas OLA and MQCA were not detectable in control. Our findings demonstrated that OLA and MQCA accumulated in large yellow croaker when it was fed with the diet containing OLA, thus imposing a potential safety risk to human health. 展开更多
关键词 large yellow croaker Pseudosciaena crocea OLAQUINDOX 3-methyl-quinoxaline-2-carboxylic acid
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