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中华鳖体液免疫应答的初步研究 被引量:11
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作者 简纪常 吴婷婷 +2 位作者 杨弘 董在杰 曹萤 《中国水产科学》 CAS CSCD 1998年第4期6-10,共5页
不同季节用嗜水性单胞杆菌免疫中华鳖所产生的体液免疫应答有所不同。7~9月,抗体效价较高,免疫后第20d,效价为1∶8,免疫后第30d,效价为1∶64,第40d为1∶128;而在冬春季免疫中华鳖后,均未检测到特异性抗体... 不同季节用嗜水性单胞杆菌免疫中华鳖所产生的体液免疫应答有所不同。7~9月,抗体效价较高,免疫后第20d,效价为1∶8,免疫后第30d,效价为1∶64,第40d为1∶128;而在冬春季免疫中华鳖后,均未检测到特异性抗体。对其进行2次免疫后,也未见抗体水平升高。还探索了纯化中华鳖血清抗体的条件和方法,并获得高纯度抗体,它与人IgM无免疫同源性。 展开更多
关键词 中华鳖 嗜水性单胞菌 体液免疫 抗体
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Chitosan Extends the Shelf-life of Filleted Tilapia (Oreochromis niloticus) During Refrigerated Storage 被引量:9
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作者 CAO Rong LIU Qi +1 位作者 YIN Bangzhong WU Biao 《Journal of Ocean University of China》 SCIE CAS 2012年第3期408-412,共5页
Shelf-life extension of aquatic products is of significant economical importance. To determine the potential effect of chitosan on the shelf-life of filleted tilapia, this study analyzed the bacterial community divers... Shelf-life extension of aquatic products is of significant economical importance. To determine the potential effect of chitosan on the shelf-life of filleted tilapia, this study analyzed the bacterial community diversity in fresh and spoiled tilapia fillets stored at (4 ± 1)℃ and examined the antimicrobial activity of chitosan against relevant bacteria isolates. Results showed that Pseudomonas (20%) and Aeromonas (16%) were abundant in fresh tilapia fillets, whereas Pseudomonas (52%), Aeromonas (32%) and Staphylococcus (12%) were dominant in the spoiled samples. Chitosan showed wide-spectrum antibacterial activity against bacteria isolated from tilapia and 5.0 g L-1 chitosan was selected for application in preservation. We further determined the shelf-life of chitosan-treated, filleted tilapia stored at (4 ± 1)℃ based on microbiological, biochemical and sensory analyses. Results showed that the shelf-life of chitosan-treated, filleted tilapia was extended to 12 d, whereas that of untreated, control samples was 6 d. These indicate that chitosan, as a natural preservative, has great application potential in the shelf-life extension of tilapia fillets. 展开更多
关键词 Oreochromis niloticus bacterial flora chitosan refrigeration shelf-life
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