AIM To determine the impact of allopurinol non-adherence as a proxy for uncontrolled disease on primary prevention of end-stage renal disease(ESRD).METHODS A cohort of 2752 patients with gout diagnosis was reconstruct...AIM To determine the impact of allopurinol non-adherence as a proxy for uncontrolled disease on primary prevention of end-stage renal disease(ESRD).METHODS A cohort of 2752 patients with gout diagnosis was reconstructed using the Québec Régie de l'assurance maladie du Québec and MedE cho administrative databases.Eligible patients were new users of allopurinol,aged 45-85,with a diagnosis of hypertension,and treated with an antihypertensive drug between 1997 and 2007.RESULTS Major risk factor for ESRD onset was chronic kidney disease at stages 1 to 3 [rate ratio(RR) = 8.00;95% confidence interval(CI):3.16-22.3 and the severity of hypertension(≥ 3 vs < 3 antihypertensives)] was a trending risk factor as a crude estimate(RR = 1.94;95%CI:0.68-5.51).Of 341 patients,cases(n = 22) and controls(n = 319),high adherence level(≥ 80%) to allopurinol therapy,compared with lower adherence level(< 80%),was associated with a lower rate of ESRD onset(RR = 0.35;95%CI:0.13-0.91).CONCLUSION Gout control seem to be associated with a significantdecreased risk of ESRD onset in hypertensive populations,further research should be conducted confirming this potential associated risk.展开更多
目的:研究运用甘露醇-腺嘌呤-磷酸盐红细胞保养液(mannitol adenine phosphate red blood cell maintenance solution,MAP)重悬去白细胞洗涤红细胞对红细胞pH值、膜蛋白CD47分子和磷脂酰丝氨酸(phosphatidylserine,PS)表达变化。方法:...目的:研究运用甘露醇-腺嘌呤-磷酸盐红细胞保养液(mannitol adenine phosphate red blood cell maintenance solution,MAP)重悬去白细胞洗涤红细胞对红细胞pH值、膜蛋白CD47分子和磷脂酰丝氨酸(phosphatidylserine,PS)表达变化。方法:随机选取10袋全血,滤除白细胞后,每袋去白细胞全血平均分成2份,分别制备成去白细胞悬浮红细胞和MAP去白细胞洗涤红细胞,分别于采血第2、7、14、21、28和35天,检测CD47蛋白平均荧光强度、PS蛋白阳性率和pH值。结果:去白细胞洗涤红细胞组CD47平均荧光强度降低,第2、7、14和21天与去白细胞悬浮红细胞第35天比较,差异有统计学意义(457.35±81.67、421.93±54.01、304.03±26.08、288.79±107.07 vs 209.81±26.17,P<0.05);去白细胞洗涤红细胞组PS蛋白阳性率逐渐升高,第2、7、14、21和35天与去白细胞悬浮红细胞第35天比较,差异有统计学意义(0.08±0.13、0.07±0.13、0.03±0.02、1.34±0.56、4.14±1.53 vs 2.29±0.31,P<0.05);相同时间点MAP去白洗涤红细胞pH值均低于去白细胞悬浮红细胞,差异有统计学意义(第2天:6.82±0.04 vs 6.9±0.09、第7天:6.64±0.04 vs 6.72±0.07、第14天:6.57±0.03 vs 6.66±0.08、第21天:6.49±0.02 vs 6.58±0.04、第35天:6.45±0.02 vs 6.52±0.03,均P<0.05;第28天:6.46±0.02 vs 6.55±0.03,P<0.01)。结论:保存期内MAP去白细胞洗涤红细胞膜CD47蛋白表达降低,PS蛋白外翻增加,损伤加重与pH相关,建议第21天为MAP去白洗涤红细胞最佳保存期。展开更多
文摘AIM To determine the impact of allopurinol non-adherence as a proxy for uncontrolled disease on primary prevention of end-stage renal disease(ESRD).METHODS A cohort of 2752 patients with gout diagnosis was reconstructed using the Québec Régie de l'assurance maladie du Québec and MedE cho administrative databases.Eligible patients were new users of allopurinol,aged 45-85,with a diagnosis of hypertension,and treated with an antihypertensive drug between 1997 and 2007.RESULTS Major risk factor for ESRD onset was chronic kidney disease at stages 1 to 3 [rate ratio(RR) = 8.00;95% confidence interval(CI):3.16-22.3 and the severity of hypertension(≥ 3 vs < 3 antihypertensives)] was a trending risk factor as a crude estimate(RR = 1.94;95%CI:0.68-5.51).Of 341 patients,cases(n = 22) and controls(n = 319),high adherence level(≥ 80%) to allopurinol therapy,compared with lower adherence level(< 80%),was associated with a lower rate of ESRD onset(RR = 0.35;95%CI:0.13-0.91).CONCLUSION Gout control seem to be associated with a significantdecreased risk of ESRD onset in hypertensive populations,further research should be conducted confirming this potential associated risk.
文摘目的:研究运用甘露醇-腺嘌呤-磷酸盐红细胞保养液(mannitol adenine phosphate red blood cell maintenance solution,MAP)重悬去白细胞洗涤红细胞对红细胞pH值、膜蛋白CD47分子和磷脂酰丝氨酸(phosphatidylserine,PS)表达变化。方法:随机选取10袋全血,滤除白细胞后,每袋去白细胞全血平均分成2份,分别制备成去白细胞悬浮红细胞和MAP去白细胞洗涤红细胞,分别于采血第2、7、14、21、28和35天,检测CD47蛋白平均荧光强度、PS蛋白阳性率和pH值。结果:去白细胞洗涤红细胞组CD47平均荧光强度降低,第2、7、14和21天与去白细胞悬浮红细胞第35天比较,差异有统计学意义(457.35±81.67、421.93±54.01、304.03±26.08、288.79±107.07 vs 209.81±26.17,P<0.05);去白细胞洗涤红细胞组PS蛋白阳性率逐渐升高,第2、7、14、21和35天与去白细胞悬浮红细胞第35天比较,差异有统计学意义(0.08±0.13、0.07±0.13、0.03±0.02、1.34±0.56、4.14±1.53 vs 2.29±0.31,P<0.05);相同时间点MAP去白洗涤红细胞pH值均低于去白细胞悬浮红细胞,差异有统计学意义(第2天:6.82±0.04 vs 6.9±0.09、第7天:6.64±0.04 vs 6.72±0.07、第14天:6.57±0.03 vs 6.66±0.08、第21天:6.49±0.02 vs 6.58±0.04、第35天:6.45±0.02 vs 6.52±0.03,均P<0.05;第28天:6.46±0.02 vs 6.55±0.03,P<0.01)。结论:保存期内MAP去白细胞洗涤红细胞膜CD47蛋白表达降低,PS蛋白外翻增加,损伤加重与pH相关,建议第21天为MAP去白洗涤红细胞最佳保存期。
