首先设计合成了一种末端带羟基的新型嘧啶衍生物UPy-OH,然后利用二环己基碳二亚胺/二甲氨基吡啶催化体系将UPy-OH共价接枝到侧链带—COOH的反应性聚氨酯PU-COOH上,制得一种新的线性聚氨酯材料PU-g-UPy。理论上,侧链UPy间可形成类似共价...首先设计合成了一种末端带羟基的新型嘧啶衍生物UPy-OH,然后利用二环己基碳二亚胺/二甲氨基吡啶催化体系将UPy-OH共价接枝到侧链带—COOH的反应性聚氨酯PU-COOH上,制得一种新的线性聚氨酯材料PU-g-UPy。理论上,侧链UPy间可形成类似共价键的四重氢键体系,促使线性PU-g-UPy形成网络状超分子结构,从而使PU-g-UPy兼具线性聚氨酯良好加工性和交联聚氨酯良好力学性能的优点。建立了UPy-OH和PU-g-UPy的合成方法,并采用所建合成方法成功制得了UPy-OH(FT-IR、1 H NMR、13 C NMR表征)和PUg-UPy(FT-IR表征),这为后续进一步研究PU-g-UPy的加工性、力学性能和生物学性能等奠定了材料基础。展开更多
Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor ac...Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor activities against A2780 cells and PC-14 cells were also evaluated.展开更多
Objective To investigate the effects of small interfering RNA （siRNA） recombinant expression vector targeting survivin gene on chemotherapy sensitivity of human colon cancer cells to 5-fluorouracil. Methods siRNA re...Objective To investigate the effects of small interfering RNA （siRNA） recombinant expression vector targeting survivin gene on chemotherapy sensitivity of human colon cancer cells to 5-fluorouracil. Methods siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cell lines LOVO. After 48 hours of transfection, cells were harvested for analysis of survivin mRNA and protein expressions using RT-PCR and Western blot. In addition, after human colon cancer cell lines were treated with Survivin siRNA and/or 5-fluorouracil, MTT assay and flow cytometry were used to analyze cell proliferation and apoptosis. Results Restriction endonuclease analysis confirmed that siRNA recombinant expression vector targeting survivin gene was successfully constructed. Inhibitory ratios of survivin mRNA and protein expressions by Survivin siRNA were 36.33% and 44.65%, respectively. Survivin siRNA combined with 5-fluorouracil significantly increased the cell proliferation inhibitory ratio and apoptosis ratio compared with 5-fluorouracil treatin~ alone （P〈0.05）. Conclusion The siRNA recombinant expression vector targeting survivin gene can inhibit the expression of survivin gene, and enhance chemotherapy sensitivity of human colon cancer cells to 5- fluorouracil.展开更多
基金supported by the National Natural Science Foundation of China(21103133)Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education+2 种基金Natural Science Foundation of Shaanxi Province,China(2015JM5224)Shaanxi Provincial Education Department Program,China(2013JK0678)National Training Programs of Innovation and Entrepreneurship for Undergraduates,China(201510702030)
文摘首先设计合成了一种末端带羟基的新型嘧啶衍生物UPy-OH,然后利用二环己基碳二亚胺/二甲氨基吡啶催化体系将UPy-OH共价接枝到侧链带—COOH的反应性聚氨酯PU-COOH上,制得一种新的线性聚氨酯材料PU-g-UPy。理论上,侧链UPy间可形成类似共价键的四重氢键体系,促使线性PU-g-UPy形成网络状超分子结构,从而使PU-g-UPy兼具线性聚氨酯良好加工性和交联聚氨酯良好力学性能的优点。建立了UPy-OH和PU-g-UPy的合成方法,并采用所建合成方法成功制得了UPy-OH(FT-IR、1 H NMR、13 C NMR表征)和PUg-UPy(FT-IR表征),这为后续进一步研究PU-g-UPy的加工性、力学性能和生物学性能等奠定了材料基础。
文摘Six aminoglucose conjugates were synthesized by the reaction of aminoglucose with 5-fluorou-racil-1-acetic acid or 5-fluorouracil-1-propanoic acid and confirmed by IR, 1H NMR and elemental analyses. Their antitumor activities against A2780 cells and PC-14 cells were also evaluated.
基金Supported by National High Technology Research and Development Program of China (2001AA218051)Educational Commission of Hubei Province of China (2005A304B09)
文摘Objective To investigate the effects of small interfering RNA （siRNA） recombinant expression vector targeting survivin gene on chemotherapy sensitivity of human colon cancer cells to 5-fluorouracil. Methods siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cell lines LOVO. After 48 hours of transfection, cells were harvested for analysis of survivin mRNA and protein expressions using RT-PCR and Western blot. In addition, after human colon cancer cell lines were treated with Survivin siRNA and/or 5-fluorouracil, MTT assay and flow cytometry were used to analyze cell proliferation and apoptosis. Results Restriction endonuclease analysis confirmed that siRNA recombinant expression vector targeting survivin gene was successfully constructed. Inhibitory ratios of survivin mRNA and protein expressions by Survivin siRNA were 36.33% and 44.65%, respectively. Survivin siRNA combined with 5-fluorouracil significantly increased the cell proliferation inhibitory ratio and apoptosis ratio compared with 5-fluorouracil treatin~ alone （P〈0.05）. Conclusion The siRNA recombinant expression vector targeting survivin gene can inhibit the expression of survivin gene, and enhance chemotherapy sensitivity of human colon cancer cells to 5- fluorouracil.
