[Objective] This study aimed to investigate the histopathological changes of hens with colibacillosis.[Method] Epidemiological investigation,clinical symptoms observation,pathological autopsy,laboratory diagnosis and ...[Objective] This study aimed to investigate the histopathological changes of hens with colibacillosis.[Method] Epidemiological investigation,clinical symptoms observation,pathological autopsy,laboratory diagnosis and paraffin-embedded tissue section were performed to diagnose the laying hens from a farm in Changli County,affected by a disease similar to pericarditis and perihepatitis in clinical symptoms.[Result] The pathogen isolated from those hens was E.coli.The histopathological changes of the diseased hens included myocardial cell degeneration and inflammatory cell infiltration in heart,fibrotic perihepatitis,necrotic hepatitis in liver,decreased lymphoid cells and inflammatory cell infiltration in spleen,lung congestion,lung room full of erythrocytes,hyaline degeneration in glomerular capillary and small intestinal epithelial cell shedding.[Conclusion] This study will provide scientific basis for the diagnosis and control of colibacillosis in poultry.展开更多
OBJECTIVE: To evaluate the estrogenic efficacy of Renshen(Radix Ginseng)(GS) on reproductive target tissues in immature mice.METHODS: One hundred and ten female immature Kunming(KM) mice, 21-day-old, were randomly ass...OBJECTIVE: To evaluate the estrogenic efficacy of Renshen(Radix Ginseng)(GS) on reproductive target tissues in immature mice.METHODS: One hundred and ten female immature Kunming(KM) mice, 21-day-old, were randomly assigned to eleven groups, 10 for each; one served as control group treated with 0.154 mg/kg estradiol valerate(EV,n = 10), the rest were treated respectively with GS intragastrically at a daily dose of 0.5,1.0, 1.5, 3.0, 6.0, 12.0, 18.0, 24.0 and 30.0 g/kg(n =10 in per group) for 7 days. The estrous cycle, uterine weight, hormone levels in circulation and histomorphology changes of uterus and vagina were scrupulously examined. The estrogen receptor(ER)α and ERβ expressions in the uterus and vagina were detected by immunohistochemistry and western blotting.RESULTS: Treatment with GS at the dose of 12.0,18.0 and 24.0 g/kg resulted significant estrogenic activity in the mice, as indicated by advanced and prolonged estrous stage and increased uterine weight(all P < 0.05). GS treatment substantially promoted development of reproductive tissue by thickening the uterine endometrium and increasing vaginal epithelial layers. In addition, treatment with GS induced significant up-regulation of ERαand ERβ expressions in reproductive tissues, and ERα up-regulation was stronger than that of ERβ.GS could raise levels of circulating estrogen, simultaneously decrease levels of luteinizing hormone and follicle-stimulating hormone(all P < 0.001)compared with the control group.CONCLUSION: Our findings suggest that GS had estrogenic effect on reproductive tissues in immature mice by stimulating biosynthesis of estrogen in circulation and up-regulating ERs.展开更多
文摘[Objective] This study aimed to investigate the histopathological changes of hens with colibacillosis.[Method] Epidemiological investigation,clinical symptoms observation,pathological autopsy,laboratory diagnosis and paraffin-embedded tissue section were performed to diagnose the laying hens from a farm in Changli County,affected by a disease similar to pericarditis and perihepatitis in clinical symptoms.[Result] The pathogen isolated from those hens was E.coli.The histopathological changes of the diseased hens included myocardial cell degeneration and inflammatory cell infiltration in heart,fibrotic perihepatitis,necrotic hepatitis in liver,decreased lymphoid cells and inflammatory cell infiltration in spleen,lung congestion,lung room full of erythrocytes,hyaline degeneration in glomerular capillary and small intestinal epithelial cell shedding.[Conclusion] This study will provide scientific basis for the diagnosis and control of colibacillosis in poultry.
基金Supported by the National Basic Research Program of China(973 Program)(Basic Research of the Eighteen Against Compatibility Theory of Traditional Chinese Medicine,No.2011CB505300,Research of the Eighteen Against Compatibility Theory of Traditional Chinese Medicine Based on Prejudiced Treatment,No.2011CB505305)
文摘OBJECTIVE: To evaluate the estrogenic efficacy of Renshen(Radix Ginseng)(GS) on reproductive target tissues in immature mice.METHODS: One hundred and ten female immature Kunming(KM) mice, 21-day-old, were randomly assigned to eleven groups, 10 for each; one served as control group treated with 0.154 mg/kg estradiol valerate(EV,n = 10), the rest were treated respectively with GS intragastrically at a daily dose of 0.5,1.0, 1.5, 3.0, 6.0, 12.0, 18.0, 24.0 and 30.0 g/kg(n =10 in per group) for 7 days. The estrous cycle, uterine weight, hormone levels in circulation and histomorphology changes of uterus and vagina were scrupulously examined. The estrogen receptor(ER)α and ERβ expressions in the uterus and vagina were detected by immunohistochemistry and western blotting.RESULTS: Treatment with GS at the dose of 12.0,18.0 and 24.0 g/kg resulted significant estrogenic activity in the mice, as indicated by advanced and prolonged estrous stage and increased uterine weight(all P < 0.05). GS treatment substantially promoted development of reproductive tissue by thickening the uterine endometrium and increasing vaginal epithelial layers. In addition, treatment with GS induced significant up-regulation of ERαand ERβ expressions in reproductive tissues, and ERα up-regulation was stronger than that of ERβ.GS could raise levels of circulating estrogen, simultaneously decrease levels of luteinizing hormone and follicle-stimulating hormone(all P < 0.001)compared with the control group.CONCLUSION: Our findings suggest that GS had estrogenic effect on reproductive tissues in immature mice by stimulating biosynthesis of estrogen in circulation and up-regulating ERs.
