如何自制物体沉浮实验器

1选用材料 1)无色透明的大塑料瓶一个(如大的饮料瓶). 2)透明的直径为15mm(能顺利通过大塑料瓶口为宜),长为50mm的小塑料瓶或小玻璃瓶一个,并自配一个合适的橡胶塞(如眼药水瓶去掉瓶嘴或自带瓶盖的针药水瓶).

从汉语之特质反思中国当代文学

中国文学是用汉语所创造,汉语由古代汉语向现代汉语的演进中,主要是在中西互动中按西方语言的模式进行,其演进在有功劳的同时,也遮蔽了汉语的作为生命体的特质。中国之文是包括西方所定义的文学在内的一切由文字形成的美,当中国之文演进成西方式的文学,有极大必要和功劳的同时,又让当代文学在文学中的地位陷入困境。中国当代文学回到汉语特质,回到包括文学在内的广义之文,是走出困境之道。

The BLOC Interactomes Form a Network in Endosomal Transport

With the identification of more than a dozen novel Hermansky-Pudlak Syndrome （HPS） proteins in vesicle trafficking in higher eukaryotes, a new class of trafficking pathways has been described. It mainly consists of three newly-defined protein com- plexes, BLOC-l, -2, and -3. Compelling evidence indicates that these complexes together with two other well-known complexes, AP3 and HOPS, play important roles in endosomal transport. The interactions between these complexes form a network in protein trafficking via endosomes and cytoskeleton. Each node of this network has intra-complex and extra-complex interactions. These complexes are connected by direct interactions between the subunits from different complexes or by indirect interactions through coupling nodes that interact with two or more subunits from different complexes. The dissection of this network facilitates the understanding of a dynamic but elaborate transport machinery in protein/membrane trafficking. The disruption of this network may lead to abnormal trafficking or defective organellar development as described in patients with Hermansky-Pudlak syndrome.

A High-Performance Silicon Electro-Optic Phase Modulator with a Triple MOS Capacitor

We propose and analyze a novel Si-based electro-optic modulator with an improved metal-oxide-semiconductor （MOS） capacitor configuration integrated into silicon-on-insulator （SOl）. Three gate-oxide layers embedded in the silicon waveguide constitute a triple MOS capacitor structure, which boosts the modulation efficiency compared with a single MOS capacitor. The simulation results demonstrate that the Vπ Lπ product is 2. 4V · cm. The rise time and fall time of the proposed device are calculated to be 80 and 40ps from the transient response curve, respectively,indicating a bandwidth of 8GHz. The phase shift efficiency and bandwidth can be enhanced by rib width scaling.

Chloroplast Genetic Engineering in Higher Plants

Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plastid genome, engineering plant metabolic system, generating transplastomic plants with higher resistance to insect, disease, drought and herbicide and bioproducing of antibodies and vaccines. In this review, the principle and operating system for chloroplast genetic engineering and its application in higher plants have been discussed.

Advances in Studies and Applications of Centroidal Voronoi Tessellations

Centroidal Voronoi tessellations(CVTs) have become a useful tool in many applications ranging from geometric modeling,image and data analysis,and numerical partial differential equations,to problems in physics,astrophysics,chemistry,and biology. In this paper,we briefly review the CVT concept and a few of its generalizations and well-known properties.We then present an overview of recent advances in both mathematical and computational studies and in practical applications of CVTs.Whenever possible,we point out some outstanding issues that still need investigating.

Studying creation of bulk elementary excitation by heaters in superfluid helium-II at low temperatures

In this paper, the obtained experimental results concerning creation of bulk elementary excitations （BEEs） in isotopically pure liquid ^4He at low temperatures -60 mK are discussed. Positive rotons＇ （R^＋-rotons） creation by a pulsed heater was studied. Signals were recorded for the following quantum processes： quantum evaporation of ^4He-atoms from the free liquid-helium surface by the BEEs of the liquid helium-Ⅱ, and BEEs reflection from the free surface back into the bulk liquid. Typical signals are shown, and ratios of signal amplitudes are evaluated. For long heater pulses from 5 to 10 μs, appearance of the second atomic cloud consisting of evaporated ^4He-atoms was observed in addition to the first atomic cloud. It is thought that the first atomic cloud of the evaporated helium atoms consists of very fast ^4He-atoms with energies ～35 K evaporated by positive rotons with the special energies ～17 K （～2ER～2×8.6 K with ER representing the roton minimum energy） corresponding to the third non-dispersive Zakharenko wave. The second cloud of slower ^4He-atoms was created by surface elementary excitations （SEEs or ripplons） possessing the special energies ～7.15 K representing the binding energy. It was assumed that such SEEs can be created by phonons incoming to the liquid surface with special energies ～6.2 K corresponding to the first non-dispersive Zakharenko wave which can interact at the liquid surface with the same phonons already reflected from the surface for long heater pulses. Also, some pulsed-heater characteristics were studied in order to better understand the features of such heaters in low temperature experiments.

A NOVEL PHYSICAL-LAYER TRANSCEIVER USED IN USB2.0 SERIAL DATA LINK

The paper proposes a novel transceiver in physical layer for high-speed serial data link based upon Universal Serial Bus (USB) 2.0, comprising transmitter and receiver. In the design, transmitter contains pre-and-main driver to satisfy slew rate of output data, receiver includes optimized topology to improve preci- sion of received data. The circuit simulation is based on Cadence’s spectre software and Taiwan Semiconduc- tor Manufacture Corporation’s library of 0.25μm mixed-signal Complementary Metal-Oxide Semiconductor (CMOS) model. The front and post-simulation results reveal that the transceiver designed can transmit and re- ceive high-speed data in 480Mbps, which is in agreement with USB2.0 specification. The chip of physi- cal-layer transceiver has been designed and implemented with 0.25μm standard CMOS technology.

Transfection and expression of exogenous gene in laying hens oviduct in vitro and in vivo

To examine whether or not the regulatory sequence of chicken ovalbumin gene can drive transgene expression specifically in hen oviduct, the authors constructed an oviduct-specific expression vector (pOV), containing 3.0 kilobases (kb) of the 5'-flanking sequence and 3.0 kb of the 3'-flanking sequence of the chicken ovalbumin gene. Jellyfish green fluorescence protein (EGFP) reporter gene and bacterial LacZ reporter gene were respectively inserted into the downstream of the 5'-regulatory region. The recombinants were named as pOVEGFP and pOVLacZ. Two transfer systems, in vitro and in vivo, were used to verify the function of the vector. In vitro, the plasmid DNA pOVEGFP and pEGFP-N1 were transfected respectively by the polyethyle-neimine procedure into the primary chicken oviduct epithelium (PCOE) and fibroblasts cells isolated from laying hens. In vivo, the recombinant vector pOVLacZ was injected into egg-laying hens via wing vein and the tissues were collected for RT-PCR analysis. The results showed that expression of pEGFP-Nl was achieved at low level in oviduct epithelial cells and at high level in fibroblasts, but that the recombinant vector was not expressed in both cells. RT-PCR analysis showed that the LacZ gene was transcribed in the oviduct, but not in the heart, liver, kidney and spleen of the injected hens. Accordingly, the β-galactosidase activity was only detected in the oviduct magnum (116.7 mU/ml) and eggs (16.47 mU/ml). These results indicated that the cloned regulation regions of chicken ovalbumin gene could drive exogenous gene expression specifically in the oviducts of hens. In vivo gene injection via wing vein may serve as a rapid production system of recombinant proteins in chicken eggs. In addition, the cultured primary oviduct cells from laying hens were not efficient temporary expression systems for analyzing the function of regulating elements of ovalbumin gene.

Flower solid modeling based on sketches

In this paper we propose a method to model flowers of solid shape. Based on (Ijiri et al., 2005)’s method, we separate individual flower modeling and inflorescence modeling procedures into structure and geometry modeling. We incorporate interactive editing gestures to allow the user to edit structure parameters freely onto structure diagram. Furthermore, we use free-hand sketching techniques to allow users to create and edit 3D geometrical elements freely and easily. The final step is to automatically merge all independent 3D geometrical elements into a single waterproof mesh. Our experiments show that this solid modeling approach is promising. Using our approach, novice users can create vivid flower models easily and freely. The generated flower model is waterproof. It can have applications in visualization, animation, gaming, and toys and decorations if printed out on 3D rapid prototyping devices.

Future of liver transplantation: Non-human primates for patient-specific organs from induced pluripotent stem cells

Strategies to fill the huge gap in supply versus demand of human organs include bioartificial organs, growing humanized organs in animals, cell therapy, and implantable bioengineered constructs. Reproducing the complex relations between different cell types, generation of adequate vasculature, and immunological complications are road blocks in generation of bioengineered organs, while immunological complications limit the use of humanized organs produced in animals. Recent developments in induced pluripotent stem cell (iPSC) biology offer a possibility of generating human, patient-specific organs in non-human primates (NHP) using patient-derived iPSC and NHP-derived iPSC lacking the critical developmental genes for the organ of interest complementing a NHP tetraploid embryo. The organ derived in this way will have the same human leukocyte antigen (HLA) profile as the patient. This approach can be curative in genetic disorders as this offers the possibility of gene manipulation and correction of the patient's genome at the iPSC stage before tetraploid complementation. The process of generation of patient-specific organs such as the liver in this way has the great advantage of making use of the natural signaling cascades in the natural milieu probably resulting in organs of great quality for transplantation. However, the inexorable scientific developments in this direction involve several social issues and hence we need to educate and prepare society in advance to accept the revolutionary consequences, good, bad and ugly.

Bacterial Cellulose Templated p-Co3O4/n-ZnO Nanocomposite with Excellent VOCs Response Performance

In this work,p-type Co3O4 decorated n-type ZnO(Co3O4/ZnO)nanocomposite was designed with the assistance of bacterial cellulose template.Phase composition,morphology and element distribution were investigated by XRD,SEM,HRTEM,EDS mapping and XPS.Volatile organic compounds(VOCs)sensing measurements indicated a noticeable improvement of response and decrease of working temperature for Co3O4/ZnO sensor,in comparison with pure ZnO,i.e.,the response towards 100 ppm acetone was 63.7(at a low working temperature of 180℃),which was 26 times higher than pure ZnO(response of 2.3 at 240℃).Excellent VOCs response characteristics could be ascribed to increased surface oxygen vacancy concentration(revealed by defect characterizations),catalytic activity of Co3O4 and the special p-n heterojunction structure,and bacterial cellulose provides a facile template for designing diverse functional heterojunctions for VOCs detection and other applications.

Estimation of an Object＇s Physical Parameter by Force Sensors of a Dual-arm Robot

We are developing a nursing-care robot for physical care tasks. The concept of this robot is to promote the cared persons by the robot to activate their own motion ability as long as possible. This may lead to the improvement of the cared person＇s movement volition and movement abilities. In order to realize safe and human friendly robot care tasks, full body manipulation is an important technology, for which it is necessary to estimate the subject＇s center of gravity from the contact positions and forces with the robot＇s two arms. In this paper, we estimate the center of gravity of object based on the contact point and the contact force estimated by force sensor on both robot arms. The position of gravity center is important to realize care tasks stably. We performed experiments and simulations for the single point contact and dual points contact cases using a cylindrical object. As a result, it is found that although some errors were recognized in the experiments compared with the simulations, the relations between the contact positions and such errors were observed. Such experimental error mainly comes from the difference of shape between the real robot and the model of the robot in simulation.

Viscoelastic Analysis of a Surface Acoustic Wave Gas Sensor Coated by a New Deposition Technique

An analysis of the response of surface acoustic wave sensors coated with polymer film based on new coating deposition （self-assemble and molecularly imprinted technology） is described and the response formulas are hence deduced. Using the real part of shear modulus, the polymer can be classified into three types： glassy film, glassy-rubbery film and rubbery film, Experimental results show that the attenuation response is in better consistence with the simulation than in Martin＇s theory, but the velocity response does not accord with the calculation exactly. Maybe it is influenced by the experimental methods and environment. In addition, simulations of gas sorption for polymer films are performed. As for glassy film, the SAW sensor response increases with increasing fihn thickness, and the relationship between the sensor response and the concentration of gas is pretty linear, while as for glassy-rubbery flint and rubbery film, the relationship between the sensor sensitivity anti concentration of gas is very complicated. The ultimately calculated results indicate that the relationship between the sensor response and frequency is not always linear due to the viscoelastic prooerties of the polymer.

Evaluation of the Influence of Extracellular Polymeric Substances on the Mass Transport of Substrate within Multispecies Biofilms

A model, for evaluating the effect of porosity and volume fraction of extracellular polymeric substances (EPS) within multispecies biofilms on the effective diffusivity, is developed and experimentally validated, based on the extraction of EPS from intact biofilms. The amount of EPS in biofilms significantly affects the effective diffusivity. For biofilms with porosity of 77%—95% in the top layers and 54%—58% in the bottom layers, the value of De/Dw decreases from 0.52—0.83 in the top layers to 0.23—0.31 in the bottom layers. Generally, the effective diffusivity in the heterotrophic/autotrophic biofilms is slightly lower than that in the heterotrophic biofilms, due to the lower porosity in the heterotrophic/autotrophic biofilms.

Involvement of clustered oyster Wnt genes in gut formation

Genes encoding Wnt ligands play important roles in organ development. The Wnt10-Wnt6-Wnt1-Wnt9 cluster widely presents in many metazoan genomes, indicating the importance of gene arrangement. Hypothesis has been proposed that they may be coordinately regulated. However, few expression correlations were identified in model animals. We analyzed the tissue expression pattern of clustered oyster Wnt10, Wnt6, Wnt1, and Wnt9 a genes in this study. The results indicated the highest expression level in adult gut system of these clustered W nt genes, except for Wnt6, which had highest expression in mantle. Further whole-mount immunofluorescence assay indicated that Wnt6 protein was restricted to gut region in oyster larvae. These results suggest the possible important role of the W nt10-Wnt6-Wnt1-Wnt9 cluster in oyster gut formation.

Efficient Mode-locked and Q-switched Nd∶YVO_4 Laser with Semiconductor Saturable Absorber Mirror

We reported an efficient diode pumped Nd∶YVO4 1 064 nm laser passively mode-locked and Q-switched by a semiconductor saturable absorber mirror(SESAM). At the incident pump power of 7.5 W, 2.81 W average output power was obtained during stable CW mode locking with a repetition rate of 111 MHz. The optical conversion efficiency was 37.5%, and the slope efficiency was 39%. So far as we know, this is the highest optical-optical conversion efficiency with a SESAM at home.

Counterworking the Operation Disturbance of a Radiators Structure due to External Actions

The operation of a complicated radiators structure creating a required field in a given area is disturbed often due to different external actions. As an example, the impact of metallic objects approaching off to an antenna system with minimal irradiation on user＇s body is analyzed. In this case, the zone itself, where the area of a weak field （a dark spot） is created, is generally not approachable for sensors installation. In order to counteract the disturbance of the system operation due to presence of different metallic objects in close proximity to the radiators, two methods based on two information sources are studied. These information sources are antennas driving currents and fields at a predetermined point outside the given area. As an example, the antenna system used in the compensation method for reducing human＇s body irradiation, to which a metal object approaches, is analyzed. The conditions of severe external actions are considered： a great metal body and a small distance from this body to the dark spot. It is shown that under these conditions of severe external actions the second method based on the field values measuring demonstrates a higher efficiency. A block scheme for structure of an automatic adjustment is suggested. The considered problem is a common one, and developers of radio circuits are faced with them constantly.