Cucurbitacin B(CUB)is a major active principle contained in the calyx melo of Cucumis melo L.The immunopotentiating effects of CuB(im,qd×5)were studied.At lower doses, CuB increased the number of peripheral blood...Cucurbitacin B(CUB)is a major active principle contained in the calyx melo of Cucumis melo L.The immunopotentiating effects of CuB(im,qd×5)were studied.At lower doses, CuB increased the number of peripheral blood T lymphocytes(0.1 mg/kg),the rate of PHA-induced lymphocyte transformation(0.2 mg/kg),the number of plaque forming cells(PFC)of the spleen(0.2 mg/kg)and the level of serum hemolysin(0.4 mg/kg).The phagocytosis of macrophages and the clearance rate of charcoal particles were enhanced only by a large dose(0.8 mg/kg).The results indicate that CuB can potentiate both cellular and humoral immune function.展开更多
Objective: To study the effects of hyperbaric air exposure on the functions of peritoneal macrophages of mice. Methods: Forty-eight mice were equally randomized to 6 groups: (1) normal air group (NA); (2) hyperbaric a...Objective: To study the effects of hyperbaric air exposure on the functions of peritoneal macrophages of mice. Methods: Forty-eight mice were equally randomized to 6 groups: (1) normal air group (NA); (2) hyperbaric air group 1 (HA1); (3) hyperbaric air group 2 (HA2); (4) hyperbaric air group 3 (HA3); (5) hyperbaric oxygen group (HO);(6) hyperbaric nitrogen group (HN). Every group was exposed to corresponding pressure for 60 min, twice a day for 3 d. Peritoneal macrophages were obtained at the corresponding time to observe the changes of phagocytosis, acid phos-phatase, antigen presentation function and the produce of NO and TNF-α. Results: Compared with those in NA group, the activity of phagocytosis, acid phosphatase, antigen presentation function and the produce of NO and TNF-a were markedly inhibited in hyperbaric oxygen group and hyperbaric air group 1 ( P < 0.05, P < 0.01) and they changed little in HN group. These changes could disappear in 3 - 5 d. Conclusion: The functions of mice peritoneal macrophages were obviously inhibited in simulated air diving environment and hyperoxia may play an important role in it.展开更多
文摘Cucurbitacin B(CUB)is a major active principle contained in the calyx melo of Cucumis melo L.The immunopotentiating effects of CuB(im,qd×5)were studied.At lower doses, CuB increased the number of peripheral blood T lymphocytes(0.1 mg/kg),the rate of PHA-induced lymphocyte transformation(0.2 mg/kg),the number of plaque forming cells(PFC)of the spleen(0.2 mg/kg)and the level of serum hemolysin(0.4 mg/kg).The phagocytosis of macrophages and the clearance rate of charcoal particles were enhanced only by a large dose(0.8 mg/kg).The results indicate that CuB can potentiate both cellular and humoral immune function.
文摘Objective: To study the effects of hyperbaric air exposure on the functions of peritoneal macrophages of mice. Methods: Forty-eight mice were equally randomized to 6 groups: (1) normal air group (NA); (2) hyperbaric air group 1 (HA1); (3) hyperbaric air group 2 (HA2); (4) hyperbaric air group 3 (HA3); (5) hyperbaric oxygen group (HO);(6) hyperbaric nitrogen group (HN). Every group was exposed to corresponding pressure for 60 min, twice a day for 3 d. Peritoneal macrophages were obtained at the corresponding time to observe the changes of phagocytosis, acid phos-phatase, antigen presentation function and the produce of NO and TNF-α. Results: Compared with those in NA group, the activity of phagocytosis, acid phosphatase, antigen presentation function and the produce of NO and TNF-a were markedly inhibited in hyperbaric oxygen group and hyperbaric air group 1 ( P < 0.05, P < 0.01) and they changed little in HN group. These changes could disappear in 3 - 5 d. Conclusion: The functions of mice peritoneal macrophages were obviously inhibited in simulated air diving environment and hyperoxia may play an important role in it.