Phage peptide libraries are collections of the specific length of short peptides,and they are based on phages as their carriers.They include mimotope libraries and peptide antibody libraries.Phage-displayed peptide li...Phage peptide libraries are collections of the specific length of short peptides,and they are based on phages as their carriers.They include mimotope libraries and peptide antibody libraries.Phage-displayed peptide libraries have been used to isolate specific ligands for numerous protein targets,and they have been proven useful in defining antigen momitopes,rapid determination of binding energetics at protein-protein interfaces,designing of vaccine and tumor research aspects.This review summarized the research progression of phage peptide library.展开更多
Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input w...Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 312 out of 360 phage clones were positive in ELISA, 1 clone shows blocking VP1 adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40,p55: GlnLeuIleMetIleArgHis; p69,p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1 receptor epitopes, one peptide can block VP1 transfection to host cell, it was a clue to study receptor’s structure and phage infectious mechanism to host cell.展开更多
基金广州市科技局科研重点立项课题 (No .2 0 0 1-Z - 0 1- 2 ) 广东省科技厅研究团队项目课题 (No .0 15 0 12 )
文摘Phage peptide libraries are collections of the specific length of short peptides,and they are based on phages as their carriers.They include mimotope libraries and peptide antibody libraries.Phage-displayed peptide libraries have been used to isolate specific ligands for numerous protein targets,and they have been proven useful in defining antigen momitopes,rapid determination of binding energetics at protein-protein interfaces,designing of vaccine and tumor research aspects.This review summarized the research progression of phage peptide library.
文摘Use Vibrio cholerae El Tor Typing Phage VP1 as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 312 out of 360 phage clones were positive in ELISA, 1 clone shows blocking VP1 adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40,p55: GlnLeuIleMetIleArgHis; p69,p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1 receptor epitopes, one peptide can block VP1 transfection to host cell, it was a clue to study receptor’s structure and phage infectious mechanism to host cell.
