本文以光学、电子显微镜对元蘑(Pleurotus oetreatus (Jacg ex Fr.)Quel子实层结构及担子发育的研究表明,元蘑的菌褶属于子实层同时发育类型,菌髓位于中央,其两侧依次是子实下层和子实层,子实层由担子,囊状体组成。担子由子实下层的菌...本文以光学、电子显微镜对元蘑(Pleurotus oetreatus (Jacg ex Fr.)Quel子实层结构及担子发育的研究表明,元蘑的菌褶属于子实层同时发育类型,菌髓位于中央,其两侧依次是子实下层和子实层,子实层由担子,囊状体组成。担子由子实下层的菌丝顶端细胞经过亚担子锁状联合形成,双核的幼担子经过核配及减效分裂,产生四个单倍体细胞核,元蘑的担子存在Duncan等提出的减数分裂后活动(post-meiotic events),表现为A型及C型,说明元蘑的减数分裂后活动是双重类型。展开更多
1杨梅简介,杨梅(Myrica rubra Sieb et Zucc),别名树梅、珠红、杨果等,是双子叶纲杨梅科常绿乔木植物,是原产中国的亚热带果树之一。野生种生长史已有7000多年,人工栽培也有2000多年。为我国南方的特产果树,主要分布在长江以南的浙、苏...1杨梅简介,杨梅(Myrica rubra Sieb et Zucc),别名树梅、珠红、杨果等,是双子叶纲杨梅科常绿乔木植物,是原产中国的亚热带果树之一。野生种生长史已有7000多年,人工栽培也有2000多年。为我国南方的特产果树,主要分布在长江以南的浙、苏、闽、粤、滇等省区,其中栽培面积、产量、品种及品质等均以浙江为最,温州主栽品种为丁岙、东魁、荸荠、晚稻梅等全国四大良种。杨梅果实呈球形,直径约2~3 cm,果肉由无数充满汁液的囊状体组成,从果心呈放射状排列。展开更多
Purpose: The aim of this study was to report the experience and efficacy of Ethibloc sclerotherapy as treatment of lymphangiomas. Methods: Between 1992 and 2004, 63 patients had Ethibloc sclerotherapy for lymphangioma...Purpose: The aim of this study was to report the experience and efficacy of Ethibloc sclerotherapy as treatment of lymphangiomas. Methods: Between 1992 and 2004, 63 patients had Ethibloc sclerotherapy for lymphangiomas at our institution. Computed tomographic scan or magnetic resonance imaging and clinical evaluation determined efficacy of the treatment. Results were classified as excellent (≥ 95% decrease in lesion volume), satisfactory (≥ 50% decrease and asymptomatic), or poor ( < 50% decrease or symptomatic). Results: Sixty- three patients with 67 lesions underwent sclerotherapy with a median of 2 treatments per patient. Thirty- five involved the neck; 10, the head and face; and 22, the thorax or limb. Thirteen were predominantly microcystic; 28, macrocystic; and 26, mixed. Of the 63 patients, 6 underwent sclerotherapy for postsurgical residual lesions. Results were classified by type: of the 54 macrocystic/mixed cases, 26 (49% ) had excellent, 19 (35% ) had good, and 9 (16% ) had poor results; in the 13 predominantly microcystic lesions, 3 (23% ) had excellent, 7 (54% ) had good, and 3 (23% ) poor results. Five patients (7.7% ) required surgery for complications; 2, for scar revision; 2, for persistent drainage; and 1, for a salivary fistula. Infection occurred in 4 patients (6.2% ) after sclerotherapy. Follow- up averaged 3.5 years (range, 12 months to 12 years). Conclusion: Ethibloc sclerotherapy is a safe and effective alternative to surgical excision of macrocystic lymphangiomas and can be used for postsurgical recurrences.展开更多
Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explan...Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explants from 10-day-old rats were cultured with TGFβ1 or TGFβ2 in the presence of FGF for 5 days, then were examined by light and electron microscopy, and by immunolocal- ization of smooth muscle(α-sm) actin and type I collagen. Results. In TGFβ/FGF-treated explants,extensive proliferation occured, with formation of spindle and star-shaped cells. These cells showed ultrastructure and biochemical features of fibroblast or myofibroblast. Prominent Golgi apparatus and rough endoplaic reticulum were observed in some cells. Intracellular micro- filaments with cytoplasmic dense babies and membrane associated dense bodies, features of smooth muscle cells, were also observed. Some cells showed reactivity to -sin actin antibody. TGFβ/FGF-treated ex- plants were strongly stained with type I collagen antibody. Condusion. In the presence of FGF, TGFβ1 and TGFβ2 induced lens epithelial cell (LEC ) proliferation and transformation into fibroblast or myofibroblast-like cells, with producing of abundant collagen matrix in the explants. The changes are similar to the metaplasia that occurrs in subcapsular opacification of the lens. The findings suggest that TGFβ and FGF plays a role in the pathogenesis of subcapsular opacification of the lens.展开更多
文摘Purpose: The aim of this study was to report the experience and efficacy of Ethibloc sclerotherapy as treatment of lymphangiomas. Methods: Between 1992 and 2004, 63 patients had Ethibloc sclerotherapy for lymphangiomas at our institution. Computed tomographic scan or magnetic resonance imaging and clinical evaluation determined efficacy of the treatment. Results were classified as excellent (≥ 95% decrease in lesion volume), satisfactory (≥ 50% decrease and asymptomatic), or poor ( < 50% decrease or symptomatic). Results: Sixty- three patients with 67 lesions underwent sclerotherapy with a median of 2 treatments per patient. Thirty- five involved the neck; 10, the head and face; and 22, the thorax or limb. Thirteen were predominantly microcystic; 28, macrocystic; and 26, mixed. Of the 63 patients, 6 underwent sclerotherapy for postsurgical residual lesions. Results were classified by type: of the 54 macrocystic/mixed cases, 26 (49% ) had excellent, 19 (35% ) had good, and 9 (16% ) had poor results; in the 13 predominantly microcystic lesions, 3 (23% ) had excellent, 7 (54% ) had good, and 3 (23% ) poor results. Five patients (7.7% ) required surgery for complications; 2, for scar revision; 2, for persistent drainage; and 1, for a salivary fistula. Infection occurred in 4 patients (6.2% ) after sclerotherapy. Follow- up averaged 3.5 years (range, 12 months to 12 years). Conclusion: Ethibloc sclerotherapy is a safe and effective alternative to surgical excision of macrocystic lymphangiomas and can be used for postsurgical recurrences.
文摘Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explants from 10-day-old rats were cultured with TGFβ1 or TGFβ2 in the presence of FGF for 5 days, then were examined by light and electron microscopy, and by immunolocal- ization of smooth muscle(α-sm) actin and type I collagen. Results. In TGFβ/FGF-treated explants,extensive proliferation occured, with formation of spindle and star-shaped cells. These cells showed ultrastructure and biochemical features of fibroblast or myofibroblast. Prominent Golgi apparatus and rough endoplaic reticulum were observed in some cells. Intracellular micro- filaments with cytoplasmic dense babies and membrane associated dense bodies, features of smooth muscle cells, were also observed. Some cells showed reactivity to -sin actin antibody. TGFβ/FGF-treated ex- plants were strongly stained with type I collagen antibody. Condusion. In the presence of FGF, TGFβ1 and TGFβ2 induced lens epithelial cell (LEC ) proliferation and transformation into fibroblast or myofibroblast-like cells, with producing of abundant collagen matrix in the explants. The changes are similar to the metaplasia that occurrs in subcapsular opacification of the lens. The findings suggest that TGFβ and FGF plays a role in the pathogenesis of subcapsular opacification of the lens.
基金Supported by the International Foundation of Science (No.C/5146-1)Natural Science Foundation of China (No.31300550)Science Foundation of Zhejiang Province (No.LY14C140005)
