The effects of carbon source, Na\|glutamate, salinity, and incubation temperature on the growth of \%Thraustochytrium aureum\% H0 and the production of docosahexaenoic acid were studies. The time course of the cell gr...The effects of carbon source, Na\|glutamate, salinity, and incubation temperature on the growth of \%Thraustochytrium aureum\% H0 and the production of docosahexaenoic acid were studies. The time course of the cell growth of \%Thraustochytrium aureum\% H0 was determined. The results show that glucose is the optimum carbon source and the optimum concentrations of glucose and Na\|glutamate are 30 g/L and 5 g/L, respectively. The suitable salinity is half of salinity of sea water. Under the optimum culture conditions, 6\^2g/L of the dry cell weight and 0\^51g/L of the docosahexaenoic acid were obtained.展开更多
为了确定破囊壶菌Aurantiochytrium sp.的最佳破壁方法,分别使用反复冻融法、超声法、高速匀浆法、碱热法和研磨法对破囊壶菌进行破壁处理。为了解各方法的破壁效果,使用显微镜观察破壁前后的细胞个数变化,并利用SEM(Scanning Electron ...为了确定破囊壶菌Aurantiochytrium sp.的最佳破壁方法,分别使用反复冻融法、超声法、高速匀浆法、碱热法和研磨法对破囊壶菌进行破壁处理。为了解各方法的破壁效果,使用显微镜观察破壁前后的细胞个数变化,并利用SEM(Scanning Electron Microscope)观察细胞的形态变化。为探究不同破壁方法对油脂提取的影响,首先使用尼罗红荧光染色法对油脂含量进行了定性检测,随后采用正己烷提取油脂。为探究不同破壁方法对油脂组分的影响,使用气相色谱法分析了油脂的组成情况。结果显示:(1)碱热的破壁效果最好,破壁率达到95.51%;(2)碱热破壁后得到的油脂产率最高,达到61.33%,远高于其他4种方法 ;(3)碱热破壁后得到的油脂中DHA含量最高、达到41.33%,相应的DHA产量也远高于其他方法、达到2.192 g/L。从制备高纯度DHA的角度出发,碱热法是最佳的破壁方法。展开更多
文摘The effects of carbon source, Na\|glutamate, salinity, and incubation temperature on the growth of \%Thraustochytrium aureum\% H0 and the production of docosahexaenoic acid were studies. The time course of the cell growth of \%Thraustochytrium aureum\% H0 was determined. The results show that glucose is the optimum carbon source and the optimum concentrations of glucose and Na\|glutamate are 30 g/L and 5 g/L, respectively. The suitable salinity is half of salinity of sea water. Under the optimum culture conditions, 6\^2g/L of the dry cell weight and 0\^51g/L of the docosahexaenoic acid were obtained.
