A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 n...A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 nucleotides obtained from a set of overlapping cDNA clones excluding poly A tail,and is comprised of 9 ORFs,two of which encode nonstructural proteins and the others encode the structural proteins. The full-length sequence of HB-1(sh)/2002 was compared with that of two Chinese isolates,BJ-4 and CH-1a.Comparative analysis presented that the complete nucleotide sequence of HB-1(sh)/2002 shared 89.8% and 96.0% identity with BJ-4 and CH-1a,respectively.Compared with the other isolates,it seemed to be more extensive in Nsp2 of nonstructural regions and ORF5 of structural coding regions,respectively.Especially,Nsp2 only shared 77.3% and 89.1% identity with BJ-4 and CH-1a.The ORF5 shared 87.0% and 93.5% identity with BJ-4 and CH-1a.Phylogenetic analysis based on Nsp2 deduced amino acid sequence suggested that HB-1(sh)/2002 belongs to the same subgroup as CH-1a, and Nsp2 can be used as basis for monitoring the genetic variation and typing the PRRSV isolates further.展开更多
文摘A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 nucleotides obtained from a set of overlapping cDNA clones excluding poly A tail,and is comprised of 9 ORFs,two of which encode nonstructural proteins and the others encode the structural proteins. The full-length sequence of HB-1(sh)/2002 was compared with that of two Chinese isolates,BJ-4 and CH-1a.Comparative analysis presented that the complete nucleotide sequence of HB-1(sh)/2002 shared 89.8% and 96.0% identity with BJ-4 and CH-1a,respectively.Compared with the other isolates,it seemed to be more extensive in Nsp2 of nonstructural regions and ORF5 of structural coding regions,respectively.Especially,Nsp2 only shared 77.3% and 89.1% identity with BJ-4 and CH-1a.The ORF5 shared 87.0% and 93.5% identity with BJ-4 and CH-1a.Phylogenetic analysis based on Nsp2 deduced amino acid sequence suggested that HB-1(sh)/2002 belongs to the same subgroup as CH-1a, and Nsp2 can be used as basis for monitoring the genetic variation and typing the PRRSV isolates further.
