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加拿大批准四价体脑膜炎球菌疫苗Menveo上市
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《齐鲁药事》 2010年第8期476-476,共1页
关键词 加拿大 四价体脑膜炎球菌疫苗 Menveo 副作用
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两个高结实率的同源四倍体水稻恢复系农艺性状及细胞遗传学比较研究 被引量:3
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作者 栾丽 龙文波 +5 位作者 王兴 陈英 刘玉花 涂升斌 孔繁伦 李万明 《应用与环境生物学报》 CAS CSCD 北大核心 2008年第1期11-17,共7页
以高结实率的同源四倍体水稻恢复系T4002和T4063为材料进行农艺性状及细胞遗传学比较研究.结果表明,T4002和T4063具有大穗、大粒、叶色浓绿、根深杆壮、株型理想、抗倒、适应力强、分蘖较好、结实率高等农艺性状.T4002和T4063染色体组... 以高结实率的同源四倍体水稻恢复系T4002和T4063为材料进行农艺性状及细胞遗传学比较研究.结果表明,T4002和T4063具有大穗、大粒、叶色浓绿、根深杆壮、株型理想、抗倒、适应力强、分蘖较好、结实率高等农艺性状.T4002和T4063染色体组成均为2n=4x=48,花粉母细胞(PMC)具有较为理想的减数分裂行为,配对染色体的比率在99%以上;其平均染色体构型分别为0.05Ⅰ+19.96Ⅱ(9.89rod+10.07ring)+0.01Ⅲ+2.20Ⅳ和0.11Ⅰ+19.17Ⅱ(8.90rod+10.37ring)+0.09Ⅲ+2.26Ⅳ+0.01Ⅵ,平均交叉分别为37.4700和37.0426.T4002和T4063PMC减数分裂各个时期单价体和三价体的比例低,而中期I(MI)PMC观察到较多二价体和四价体,其最大频率的染色体构型分别为12II6IV和10II7IV.MI单价体、三价体和多价体频率,后期I(AI)、末期I(TI)和末期II(TII)异常染色体行为都与花粉育性和结实率呈负相关.AI染色体滞后细胞比率同TI异常细胞比率呈极显著正相关,表明AI染色体滞后是TI微核形成的主要原因.多价体数目与TII异常形成四分孢子的PMC比率显著相关,表明多价体不仅影响AI(相关系数0.72)和TI(相关系数0.79),且显著影响减数分裂II染色体分配,从而影响TII正常四分小孢子形成.AI染色体滞后细胞比率同TI异常细胞比率呈极显著正相关,暗示影响AI染色体分离及TI微核形成的基因很可能是显性单基因. 展开更多
关键词 同源水稻 恢复系 减数分裂 四价体 相关分析
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小鼠睾丸初级精母细胞染色体畸变的意义和构型过程
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作者 姚小曼 《卫生研究》 CAS CSCD 北大核心 1995年第S2期41-44,共4页
初级精母细胞染色体受损伤,其三价体、四价体的形成必须经过DNA复制使损伤插入到DNA中才能形成多价体,了解此构型过程对正确诊断畸变类型颇有帮助。
关键词 初级精母细胞 染色 四价体
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核电站排放氚的生殖毒理学实验研究
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作者 周湘艳 董金婵 +1 位作者 姜涛 王春燕 《毒理学杂志》 CAS CSCD 1993年第S1期108-111,共4页
在核能运转中,氚的释放成为目前辐射防护中关注的问题。氚释放到环境中,大多成水的形式存在,通过食物链极易进入体内,给人类健康及后代带来的潜在危害是不可忽视的。
关键词 染色畸变 辐射防护 精原细胞 生殖毒理学 遗传性缺陷 效应关系 中毒方式 基因突变 四价体
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黑麦 小黑麦 燕麦
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《麦类文摘》 1994年第2期33-33,共1页
Z940399 N<sup>+</sup>离子引起的黑麦染色体易位/朱凤绥(中国农科院作物所),陆挺…//作物学报.-1993,19(4).-299~303离子注入黑麦的生物效应,有生理变异。也有遗传变异,经过低能 N<sup>+</sup>离子束辐... Z940399 N<sup>+</sup>离子引起的黑麦染色体易位/朱凤绥(中国农科院作物所),陆挺…//作物学报.-1993,19(4).-299~303离子注入黑麦的生物效应,有生理变异。也有遗传变异,经过低能 N<sup>+</sup>离子束辐照。选出黑麦284-2,其减数分裂的染色体观察,在中期Ⅰ和终变期,所有细胞均出现1个相邻式环状四价体或交替式∞状四价体,其比例为1:1,M<sub>1</sub>代体细胞染色体出现2对染色体畸变,经 C 分带的带型分析,获得了1R 和4R 的染色体易位。还提出了黑麦284-2由杂合易位体获得纯合易位体的途径,探讨了离子束辐照诱变植物的机理。(参12) 展开更多
关键词 染色易位 四价体 细胞染色 染色畸变 遗传变异 终变期 作物学报 邻式 减数分裂 纯合
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Preferential Bivalent Formation in Tetraploid Male of Pacific Oyster Crassostrea gigas Thunberg
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作者 ZHANG Zhengrui WANG Xinglian +1 位作者 ZHANG Quanqi Standish Allen Jr. 《Journal of Ocean University of China》 SCIE CAS 2014年第2期297-302,共6页
Artificially induced tetraploid Pacific oyster, Crassostrea gigas Thunberg, produces more aneuploid gametes than nor- mal diploid one, although they showed a comparable fecundity to diploidy. The meiotic chromosome co... Artificially induced tetraploid Pacific oyster, Crassostrea gigas Thunberg, produces more aneuploid gametes than nor- mal diploid one, although they showed a comparable fecundity to diploidy. The meiotic chromosome configuration of 3 tetraploid and 1 tetraploid/triploid mosaic males were analyzed through direct chromosome observation. A majority of metaphase I spermato- cytes contained both bivalents and quadrivalents. The chromosome configuration of these males was characterized by preferential formation of bivalents to quadrivalents. Bivalents appeared in all spermatocytes and consisted of 86% of all chromosome aggregates. In comparison, quadrivalents occurred in 91% spermatocytes and consisted of only 12.6% of all chromosome aggregates. The mean bivalent frequency per spermatocyte varied between 14.4 and 17.2; while that of quadrivalents varied between 2.2 and 2.7. Most quadrivalents were tandemly chained (58%) or circled (39%). The total number of chromosome aggregates per spermatocyte ranged from 13 to 20 with an average of 17.6; while 18 (16 bivalents and 2 quadrivalents) was the most frequent. Univalents and trivalents appeared in very low frequency. Aneuploid (hypotetraploid) spermatocytes were observed in a low frequency. The chromosome con- figuration of in the mosaic individual was similar to that of tetraploid individuals. The percentage of triploid spermatocytes (2%) of the mosaic individual was significantly lower (2"2 =30, P〈 0.01) than that of triploid cells (46%) in its somatic tissue. 展开更多
关键词 tetraploid oyster MEIOSIS BIVALENT quadrivalent REVERSION
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Experimental Evaluation of the Post-ultimate Strength Behavior of a Ship's Hull Girder in Waves 被引量:6
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作者 Kazuhiro Iijima Ryota Wada Masahiko Fujikubo 《Journal of Marine Science and Application》 2012年第1期34-43,共10页
Experimental investigations into the collapse behavior of a box-shape hull girder subjected to extreme wave-induced loads are presented.The experiment was performed using a scaled model in a tank.In the middle of the ... Experimental investigations into the collapse behavior of a box-shape hull girder subjected to extreme wave-induced loads are presented.The experiment was performed using a scaled model in a tank.In the middle of the scaled model,sacrificial specimens with circular pillar and trough shapes which respectively show different bending moment-displacement characteristics were mounted to compare the dynamic collapse characteristics of the hull girder in waves.The specimens were designed by using finite element(FE)-analysis.Prior to the tank tests,static four-point-bending tests were conducted to detect the load-carrying capacity of the hull girder.It was shown that the load-carrying capacity of a ship including reduction of the capacity after the ultimate strength can be reproduced experimentally by employing the trough type specimens.Tank tests using these specimens were performed under a focused wave in which the hull girder collapses under once and repetitive focused waves.It was shown from the multiple collapse tests that the increase rate of collapse becomes higher once the load-carrying capacity enters the reduction path while the increase rate is lower before reaching the ultimate strength. 展开更多
关键词 post-ultimate strength collapse behavior experimental evaluation load-carrying capacity hull girder
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流感疫苗病毒株在MDCK细胞中的培养条件 被引量:4
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作者 李贝贝 罗剑 +1 位作者 黄海武 周琳婷 《国际生物制品学杂志》 CAS 2018年第6期261-265,共5页
目的 研究4价流感疫苗所用病毒株在MDCK细胞中的扩增条件.方法 在6孔细胞培养板中以不同感染复数(multiplicity of infection,MOI)(0.1000、0.0100、0.0010、0.0001)和对甲苯磺酰-L-苯丙氨酸氯甲基酮(TPCK)-胰蛋白酶(胰酶)浓度(0、2、4... 目的 研究4价流感疫苗所用病毒株在MDCK细胞中的扩增条件.方法 在6孔细胞培养板中以不同感染复数(multiplicity of infection,MOI)(0.1000、0.0100、0.0010、0.0001)和对甲苯磺酰-L-苯丙氨酸氯甲基酮(TPCK)-胰蛋白酶(胰酶)浓度(0、2、4、8μg/ml)进行病毒接种和扩增,感染后72 h收获细胞上清液,检测病毒血凝素效价,确定病毒最佳扩增条件.随后,在搅拌瓶中进行MDCK细胞微载体悬浮培养,研究不同起始细胞接种密度(1.5×105、2.0×105、3.0×105个/ml)和微载体浓度(3、5、10 g/L)对MDCK细胞生长的影响,确定细胞最佳扩增条件.根据确定的最佳扩增条件,在搅拌瓶培养体系中扩增4种病毒.结果 6孔板中4种流感病毒的最佳接种条件分别是,A/Michigan/45/2015(H1N1)pdm09:MOI 0.0100、TPCK-胰酶浓度2μg/ml;A/Hongkong/4801/2014(H3N2):MOI 0.0100、TPCK-胰酶浓度4μg/ml;B/Brisbane/60/2008:MOI 0.0010、TPCK-胰酶浓度4μg/ml;B/Phuket/3073/2013:MOI 0.0100、TPCK-胰酶浓度4μg/ml.在搅拌瓶中以3.0×105个/ml初始细胞密度接种,3 g/L微载体浓度培养,MDCK细胞能够实现较好的扩增,最高密度可达2.1×106个/ml;搅拌瓶悬浮培养,以最佳接种条件接种后,4种病毒的血凝素效价为6.75~8.42 log2血凝素单位/50μl.结论 通过摸索病毒接种最佳MOI、TPCK-胰酶浓度及优化MDCK细胞微载体悬浮培养条件,能够在MDCK细胞微载体悬浮培养体系中有效扩增4价流感疫苗用病毒株,为后期工艺放大研究奠定基础. 展开更多
关键词 流感疫苗 四价体 Madin-Darby犬肾细胞 微载
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Twice Q-polynomial distance-regular graphs of diameter 4
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作者 MA JianMin KOOLEN Jack H. 《Science China Mathematics》 SCIE CSCD 2015年第12期2683-2690,共8页
It is known that a distance-regular graph with valency k at least three admits at most two Qpolynomial structures. We show that all distance-regular graphs with diameter four and valency at least three admitting two Q... It is known that a distance-regular graph with valency k at least three admits at most two Qpolynomial structures. We show that all distance-regular graphs with diameter four and valency at least three admitting two Q-polynomial structures are either dual bipartite or almost dual bipartite. By the work of Dickie(1995) this implies that any distance-regular graph with diameter d at least four and valency at least three admitting two Q-polynomial structures is, provided it is not a Hadamard graph, either the cube H(d, 2)with d even, the half cube 1/2H(2d + 1, 2), the folded cube?H(2d + 1, 2), or the dual polar graph on [2A2d-1(q)]with q 2 a prime power. 展开更多
关键词 distance-regular graph P-or Q-polynomial structure TIGHT
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