目的:建立测定四取代四苯基卟啉衍生物(TLTAPP)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters Sun Fire^(TM)C_(18),流动相A为乙腈-水(含0.1%三氟乙酸,95∶5,V/V)、流动相B为乙腈-水(含0.1%三氟乙酸,5∶95,V/V)(梯度洗脱),...目的:建立测定四取代四苯基卟啉衍生物(TLTAPP)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters Sun Fire^(TM)C_(18),流动相A为乙腈-水(含0.1%三氟乙酸,95∶5,V/V)、流动相B为乙腈-水(含0.1%三氟乙酸,5∶95,V/V)(梯度洗脱),流速为1.0 ml/min,柱温为30℃,检测波长为418 nm,进样量为20μl。结果:TLTAPP检测质量浓度线性范围为1.55~95μg/ml(r=0.999 4);检测限和定量限分别为1.1 ng和3.8 ng;精密度、稳定性、重复性试验的RSD<1%;加样回收率为99.00%~101.60%,RSD=0.89%(n=9)。结论:该方法简单、快速、准确、专属性强,可用于TLTAPP的含量测定。展开更多
Tetra\{\[4\|carboxymethoxyphenyl\]\}porphyrin copper(Ⅱ), nickel(Ⅱ), zinc(Ⅱ) complex and their dibutyltin(Ⅳ) ester derivatives have been synthesized and characterized. The equilibrium constants of the aggregation o...Tetra\{\[4\|carboxymethoxyphenyl\]\}porphyrin copper(Ⅱ), nickel(Ⅱ), zinc(Ⅱ) complex and their dibutyltin(Ⅳ) ester derivatives have been synthesized and characterized. The equilibrium constants of the aggregation of the metal porphyrin and the equilibrium constant of the π\|π coordination with phenothoroline have been measured by using UV\|Vis titration method. The results show that the equilibrium constants decrease in the order of K \-\{CuP\}> K \-\{NiP\}> K \-\{ZnP\}. The vitro anti\|tumor activities of the dibutyltin(Ⅳ) metal porphyrinate has some relation with the ability of aggregation and coordination with ligand of the metal porphyrin.展开更多
文摘Tetra\{\[4\|carboxymethoxyphenyl\]\}porphyrin copper(Ⅱ), nickel(Ⅱ), zinc(Ⅱ) complex and their dibutyltin(Ⅳ) ester derivatives have been synthesized and characterized. The equilibrium constants of the aggregation of the metal porphyrin and the equilibrium constant of the π\|π coordination with phenothoroline have been measured by using UV\|Vis titration method. The results show that the equilibrium constants decrease in the order of K \-\{CuP\}> K \-\{NiP\}> K \-\{ZnP\}. The vitro anti\|tumor activities of the dibutyltin(Ⅳ) metal porphyrinate has some relation with the ability of aggregation and coordination with ligand of the metal porphyrin.
