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浅谈《孟姜女》“四季体”的创作与演唱 被引量:2
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作者 苏漪 《黄河之声》 2016年第21期8-10,共3页
《孟姜女》是以我国流传已久的爱情故事"孟姜女哭长城"为背景,由王志信在民间小调《孟姜女》的基础上改编而来的现代民族声乐作品。将歌曲原本的"十二月体"重新裁剪成为"四季体",以四季的变化来展示故事... 《孟姜女》是以我国流传已久的爱情故事"孟姜女哭长城"为背景,由王志信在民间小调《孟姜女》的基础上改编而来的现代民族声乐作品。将歌曲原本的"十二月体"重新裁剪成为"四季体",以四季的变化来展示故事的情节。本文则以四个季节为轴,分别展开阐述了歌曲的曲式和声、人物特点、情感表现及演唱要求。 展开更多
关键词 《孟姜女》 四季体 历史故事 演唱技巧 人物特点
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Cloning and Expression of C4B Gene in Siji Goose
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作者 茆骏 邢光东 王根林 《Agricultural Science & Technology》 CAS 2014年第1期17-20,共4页
[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple s... [Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases. 展开更多
关键词 Siji goose COMPLEMENT C4B gene CLONING
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