The typical representative TPB(tetraphenyl-1,3-butadiene)molecule with AIE effect was used as a fluorescent probe,and the specific recognition of tetracycline antibiotics was achieved through the comparison of the exp...The typical representative TPB(tetraphenyl-1,3-butadiene)molecule with AIE effect was used as a fluorescent probe,and the specific recognition of tetracycline antibiotics was achieved through the comparison of the experimental results of the quenching of TPB by nine antibiotics of different types,which indicated that tetracycline antibiotics were found to quench TPB to the greatest extent,while the quenching of other antibiotics(e.q.chloramphenicol,aminoglycoside,sulfonamides,and quinolone)was not obvious.Then the quantitative relationship between the concentration of antibiotic solution and the effect of fluorescence quench was investigated by taking oxytetracycline among tetracycline antibiotics as an example,and the detection limit was calculated.Finally,it was found that the UV absorption spectra of tetracycline hydrochloride and the fluorescence excitation spectra of TPB had high overlap,while the overlap between the UV absorption spectra of other antibiotics,such as sulfadimethoxine,and the fluorescence laser spectrum of TPB was low,which fulfilled the fluorescence internal filtering effect.The fluorescence probe has stable properties,low cost and strong anti-interference ability,which makes the method more valuable in the practical detection of tetracycline.This study indicates great potent in the detection of antibiotics.展开更多
基金supported by the National Natural Science Foundation of China(22109105,22102106)Innovation Entrepreneurship Training Program for College Students of the School of Chemistry and Chemical Engineering at Shenyang Normal University
文摘The typical representative TPB(tetraphenyl-1,3-butadiene)molecule with AIE effect was used as a fluorescent probe,and the specific recognition of tetracycline antibiotics was achieved through the comparison of the experimental results of the quenching of TPB by nine antibiotics of different types,which indicated that tetracycline antibiotics were found to quench TPB to the greatest extent,while the quenching of other antibiotics(e.q.chloramphenicol,aminoglycoside,sulfonamides,and quinolone)was not obvious.Then the quantitative relationship between the concentration of antibiotic solution and the effect of fluorescence quench was investigated by taking oxytetracycline among tetracycline antibiotics as an example,and the detection limit was calculated.Finally,it was found that the UV absorption spectra of tetracycline hydrochloride and the fluorescence excitation spectra of TPB had high overlap,while the overlap between the UV absorption spectra of other antibiotics,such as sulfadimethoxine,and the fluorescence laser spectrum of TPB was low,which fulfilled the fluorescence internal filtering effect.The fluorescence probe has stable properties,low cost and strong anti-interference ability,which makes the method more valuable in the practical detection of tetracycline.This study indicates great potent in the detection of antibiotics.
