The UVA spectra and the reaction of 5-(p-Leuphenyl)-10,15,20-triphenylporphyrin[H 2(p-Leu)TPP]adsorbed on AgCl colloid in two different solvent-anhydrous alcohol and glacial acetic acid were studied.The experimental r...The UVA spectra and the reaction of 5-(p-Leuphenyl)-10,15,20-triphenylporphyrin[H 2(p-Leu)TPP]adsorbed on AgCl colloid in two different solvent-anhydrous alcohol and glacial acetic acid were studied.The experimental results show that H 2(p Leu)TPP exists in equilibrum between the diacid form H 2(p Leu)TPP 2+ and the free base H 2(p Leu)TPP.At pH=9 5-11 0 the H 2(p-Leu)TPP is converted into Ag(Ⅱ)(p Leu)TPP on AgCl colloids.展开更多
目的:建立测定四取代四苯基卟啉衍生物(TLTAPP)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters Sun Fire^(TM)C_(18),流动相A为乙腈-水(含0.1%三氟乙酸,95∶5,V/V)、流动相B为乙腈-水(含0.1%三氟乙酸,5∶95,V/V)(梯度洗脱),...目的:建立测定四取代四苯基卟啉衍生物(TLTAPP)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters Sun Fire^(TM)C_(18),流动相A为乙腈-水(含0.1%三氟乙酸,95∶5,V/V)、流动相B为乙腈-水(含0.1%三氟乙酸,5∶95,V/V)(梯度洗脱),流速为1.0 ml/min,柱温为30℃,检测波长为418 nm,进样量为20μl。结果:TLTAPP检测质量浓度线性范围为1.55~95μg/ml(r=0.999 4);检测限和定量限分别为1.1 ng和3.8 ng;精密度、稳定性、重复性试验的RSD<1%;加样回收率为99.00%~101.60%,RSD=0.89%(n=9)。结论:该方法简单、快速、准确、专属性强,可用于TLTAPP的含量测定。展开更多
The photophysical properties of ortho-Cl, meta-Cl and para-Cl substituted tetraphenylporphy-rin-histidine and their zinc (Ⅱ) complexes have been studied by means of steady-state absorption and fluo-rescence spectrosc...The photophysical properties of ortho-Cl, meta-Cl and para-Cl substituted tetraphenylporphy-rin-histidine and their zinc (Ⅱ) complexes have been studied by means of steady-state absorption and fluo-rescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. For the cases of both free-base and zinc complexes, it was found that the or-tho-chlorine substitution onto the phenyl rings signifi-cantly altered the fluorescence quantum yield, the fluorescence lifetime and the ratio between radiative and nonradiative deactivation rates of the porphyrin chromophore, i.e. the photophysical parameters werequite different from those of meta- and para-substi-tuted compounds. On the other hand, however, theintroduction of covalently-linked histidine did not ex-ert much effects on the photophysical behavior of the porphyrin chromophore. The results are interpreted in terms of the steric effect and the heavy-atom effect from the chlorine atoms substituted onto the phenylrings.展开更多
文摘The UVA spectra and the reaction of 5-(p-Leuphenyl)-10,15,20-triphenylporphyrin[H 2(p-Leu)TPP]adsorbed on AgCl colloid in two different solvent-anhydrous alcohol and glacial acetic acid were studied.The experimental results show that H 2(p Leu)TPP exists in equilibrum between the diacid form H 2(p Leu)TPP 2+ and the free base H 2(p Leu)TPP.At pH=9 5-11 0 the H 2(p-Leu)TPP is converted into Ag(Ⅱ)(p Leu)TPP on AgCl colloids.
基金supported by the Nationa1 Natural Science Foundation of China(Grant Nos.20072041 and 20133020)the State Kcy Basis Research and Development Plans(Grant No.G1998010100).
文摘The photophysical properties of ortho-Cl, meta-Cl and para-Cl substituted tetraphenylporphy-rin-histidine and their zinc (Ⅱ) complexes have been studied by means of steady-state absorption and fluo-rescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. For the cases of both free-base and zinc complexes, it was found that the or-tho-chlorine substitution onto the phenyl rings signifi-cantly altered the fluorescence quantum yield, the fluorescence lifetime and the ratio between radiative and nonradiative deactivation rates of the porphyrin chromophore, i.e. the photophysical parameters werequite different from those of meta- and para-substi-tuted compounds. On the other hand, however, theintroduction of covalently-linked histidine did not ex-ert much effects on the photophysical behavior of the porphyrin chromophore. The results are interpreted in terms of the steric effect and the heavy-atom effect from the chlorine atoms substituted onto the phenylrings.
