AIM:To use leptin-deficient(ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification(ASQ) mode and the associated analytical parameter...AIM:To use leptin-deficient(ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification(ASQ) mode and the associated analytical parameter,"focal disturbance ratio"(FD-ratio).METHODS:Nine ob/ob mice,at 5,8,and 12 wk of age(n = 3 in each age group),were used as models for hepatic steatosis.Echo signals obtained from ultrasonography in the mice were analyzed by ASQ,which uses a statistical analysis of echo amplitude to estimate inhomogeneity in the diagnostic region.FD-ratio,as calculated from this analysis,was the focus of the present study.FD-ratio and fat droplet areas and sizes were compared between age groups.RESULTS:No fibrosis or inflammation was observed in any of the groups.The fat droplet area significantly(P < 0.01) increased with age from 1.25% ± 0.28% at 5 wk to 31.07% ± 0.48% at 8 wk to 51.69% ± 3.19% at 12 wk.The median fat droplet size also significantly(P < 0.01) increased with age,from 1.33(0.55-10.52) m at 5 wk,2.82(0.61-44.13) m at 8 wk and 6.34(0.66-81.83) m at 12 wk.The mean FD-ratio was 0.42 ± 0.11 at 5 wk,0.11 ± 0.05 at 8 wk,and 0.03 ± 0.02 at 12 wk.The FD-ratio was significantly lower at 12 wk than at 5 wk and 8 wk(P < 0.01).A significant negative correlation was observed between the FD-ratio and either the fat droplet area(r =-0.7211,P = 0.0017) or fat droplet size(r =-0.9811,P = 0.0052).CONCLUSION:This tool for statistical analysis of signals from ultrasonography using the FD-ratio can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis,and may serve as a quantitative biomarker of hepatic steatosis.展开更多
With the challenge of great growing of services diversity,service-oriented supporting ability is required by current high-speed passive optical network( PON). Aimed at enhancing the quality of service( Qo S) brought b...With the challenge of great growing of services diversity,service-oriented supporting ability is required by current high-speed passive optical network( PON). Aimed at enhancing the quality of service( Qo S) brought by diversified-services,this study proposes an echo state network( ESN)based multi-service awareness mechanism in 10-Gigabite ethernet passive optical network( 10GEPON). In the proposed approach,distributed architecture is adopted to realize this ESN based multi-service awareness. According to the network architecture of 10G-EPON,where a main ESN is running in OLT and a number of ESN agents works in ONUs. The main-ESN plays the main function of service-awareness from the total view of various kinds of services in 10G-EPON system,by full ESN training. Then,the reservoir information of well-trained ESN in OLT will be broadcasted to all ONUs and those ESN agents working in ONUs are allowed to conduct independent service-awareness function. Thus,resources allocation and transport policy are both determined only in ONUs. Simulation results show that the proposed mechanism is able to better support the ability of multiple services.展开更多
文摘AIM:To use leptin-deficient(ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification(ASQ) mode and the associated analytical parameter,"focal disturbance ratio"(FD-ratio).METHODS:Nine ob/ob mice,at 5,8,and 12 wk of age(n = 3 in each age group),were used as models for hepatic steatosis.Echo signals obtained from ultrasonography in the mice were analyzed by ASQ,which uses a statistical analysis of echo amplitude to estimate inhomogeneity in the diagnostic region.FD-ratio,as calculated from this analysis,was the focus of the present study.FD-ratio and fat droplet areas and sizes were compared between age groups.RESULTS:No fibrosis or inflammation was observed in any of the groups.The fat droplet area significantly(P < 0.01) increased with age from 1.25% ± 0.28% at 5 wk to 31.07% ± 0.48% at 8 wk to 51.69% ± 3.19% at 12 wk.The median fat droplet size also significantly(P < 0.01) increased with age,from 1.33(0.55-10.52) m at 5 wk,2.82(0.61-44.13) m at 8 wk and 6.34(0.66-81.83) m at 12 wk.The mean FD-ratio was 0.42 ± 0.11 at 5 wk,0.11 ± 0.05 at 8 wk,and 0.03 ± 0.02 at 12 wk.The FD-ratio was significantly lower at 12 wk than at 5 wk and 8 wk(P < 0.01).A significant negative correlation was observed between the FD-ratio and either the fat droplet area(r =-0.7211,P = 0.0017) or fat droplet size(r =-0.9811,P = 0.0052).CONCLUSION:This tool for statistical analysis of signals from ultrasonography using the FD-ratio can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis,and may serve as a quantitative biomarker of hepatic steatosis.
基金Supported by the National High Technology Research and Development Programme of China(No.2012AA050804)
文摘With the challenge of great growing of services diversity,service-oriented supporting ability is required by current high-speed passive optical network( PON). Aimed at enhancing the quality of service( Qo S) brought by diversified-services,this study proposes an echo state network( ESN)based multi-service awareness mechanism in 10-Gigabite ethernet passive optical network( 10GEPON). In the proposed approach,distributed architecture is adopted to realize this ESN based multi-service awareness. According to the network architecture of 10G-EPON,where a main ESN is running in OLT and a number of ESN agents works in ONUs. The main-ESN plays the main function of service-awareness from the total view of various kinds of services in 10G-EPON system,by full ESN training. Then,the reservoir information of well-trained ESN in OLT will be broadcasted to all ONUs and those ESN agents working in ONUs are allowed to conduct independent service-awareness function. Thus,resources allocation and transport policy are both determined only in ONUs. Simulation results show that the proposed mechanism is able to better support the ability of multiple services.
