丹参提取方法的比较研究

目的:对丹参的回流提取工艺法与超声提取工艺法进行比较研究。方法:用超声法提取丹参中丹酚酸B,用高效液相(HPLC)法测定丹酚酸B的含量。色谱柱为Diamonsil-C_(18)(250 mm×4.6 mm,5μm),流动相:甲醇-乙腈-甲酸-水(30:10:1:59)为流动相;流速:0.8 mL/min;柱温:30℃;检测波长为286 nm。结果:超声提取法丹酚酸B的含量为4.21%,高于2005版《中国药典》回流方法。结论:超声提取工艺法比回流提取法简单易行,节省时间,丹酚酸B的提取效率高。

止咳平喘中药中麻黄的提取方法优选

目的:优选麻黄最佳提取方法和提取工艺。方法:以麻黄提取物中麻黄碱含量为指标,考察水煎法、乙醇回流法和盐酸乙醇回流法经干燥的提取物中提取麻黄碱的多寡,并采用三水平四因素(盐酸摩尔浓度、盐酸乙醇倍数、回流时间、提取次数)正交试验法对盐酸乙醇提取条件进行优选。结果:最佳方法是盐酸乙醇回流法。最佳条件是:乙醇中的盐酸浓度为0.001mol/L,盐酸乙醇倍数为5倍、回流时间为1h,提取次数为4次。结论:每20g麻黄粗粉提取物中盐酸麻黄碱总量达0.19g,方法简便易行。

A Comparative Study of Process Parameter Optimization and Process of Flavonoid in Planted Trollius Chinensis through Backflow Method, Ultrasonic Wave Method and MicrowaveMethod

It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.