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Molecular Cloning and Construction of agp Gene Deletion-mutant in Cyanobacterium Synechocystis sp. PCC 6803 被引量:1
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作者 吴桂芳 沈忠耀 +1 位作者 吴庆余 赵南明 《Acta Botanica Sinica》 CSCD 2001年第5期512-516,共5页
The agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis was amplified by PCR. The resulting agp fragment was cloned in plasmid pUC118 to generate plasmid pUCA. Part of the... The agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis was amplified by PCR. The resulting agp fragment was cloned in plasmid pUC118 to generate plasmid pUCA. Part of the fragment within the agp DNA was deleted and replaced by an erythromycin resistance cassette to generate plasmid pUCAE, which was used to transform the Synechocystis sp. PCC 6803 wild-type strain and a mutant with resistance to erythromycin was obtained. PCR analysis of the genomic DNA from the resulting mutant indicated that the appropriate deletion and insertion indeed had occurred. The cell growth and Chl a, glycogen content in the mutant showed difference from those in the wild-type strain. The obtained biomass as well as the Chl a content in the mutant strain was higher than that of the wild-type strain, which suggested that the photosynthesis efficiency in the agp(-) strain was higher than that in the wild-type strain. No glycogen was found in the mutant, providing evidence for the correction of the mutant in physiological level. 展开更多
关键词 CYANOBACTERIUM Synechocystis sp PCC 6803 agp cloning deletion mutant glycogen synthesis photosynthesis
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广原斋读书札记——我释我联
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作者 马有林 《书法》 2008年第4期42-43,共2页
“广原斋”乃吾附庸风雅之书斋名。因姓马且属羊,“马”、“羊”皆为草食性动物,故友人在席间为我觅得这一雅号。寓意原野广阔,草肥水美能得膘肥体壮;又挥毫泼墨,蕴广原之境,放意骋怀,自然涵咏胸襟、大气磅礴。临池学书,经年二十寒暑,... “广原斋”乃吾附庸风雅之书斋名。因姓马且属羊,“马”、“羊”皆为草食性动物,故友人在席间为我觅得这一雅号。寓意原野广阔,草肥水美能得膘肥体壮;又挥毫泼墨,蕴广原之境,放意骋怀,自然涵咏胸襟、大气磅礴。临池学书,经年二十寒暑,个中甘苦,自不待言。渐学渐悟中,始知书艺之大智者莫不技法精良、学养渊深,通会之际,德艺双馨,人书俱老。为书之道,青年恃才气,中年比功力,老年看学养。学养之资在于读书识理,养气修德。不读书不足以尽广大,不修德不足以致精微。 展开更多
关键词 学养 广原 读书 德艺双馨 因姓
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