山地公园因山构园手法探析——以福建龙岩仙宫山公园为例

因山构园既是人对自然山形地势"天人合一"思想内核的一脉相承,又是技法上不断尝试和总结的过程。"因山"体现为遵循山形地势、因地制宜的谋略布局思想,而"构园"则是挖掘地域文化,结合场地特征,满足功能需求,结合现代景观生态学、海绵城市等一系列新措施,构建出一个层次分明、步移景异,有山地特色肌理的公园手法。基于福建龙岩仙宫山规划设计实践,探索因山构园设计手法,为山地公园的规划设计提供有意义的借鉴。

“因山就势”：探索贵州特色小镇发展路径

贵州是典型的山地省,山多地少,城镇分布零散、基础设施薄弱。特色小镇建设可补齐城镇体系的短板,拓展城镇化增长的内生空间,是促进就地就近城镇化的重要平台。本文介绍了贵州特色小镇发展的概况,基于增长极和点轴理论,从空间发展的视角提出特色小镇发展应创建一种可产生极化效应和发挥辐射功能的牵引力,走层次分明、重点突出的"点—线—面"非均衡发展的道路,以推动就地就近城镇化。要实现这个目标,突破口在于:因势利导地发挥贵州在大数据、大生态、大旅游、大健康、大扶贫等方面的政策优势,以人为本地补齐城镇基础设施、公共服务的短板,因地制宜地寻找和发挥比较优势,打造出特色产业小镇、特定功能小镇、特有资源小镇,走符合贵州实际的特色发展道路。

碧海青山一明珠——记连云港市海滨中学

连云港市海滨中学座落于连云港港区。她背依巍巍云台山,面向涛涛黄海,校园因山就势而造,别具匠心,园内绿树成荫,花香宜人。 大批的有用人才从海滨中学这块清新优雅的学教乐土建上社会。多年来,“勤奋、守纪、文明、健美”的优良校风,“严谨善教、教书育人”的优良教风,“勤学进取。

Construction and Identification of a Goat Pox Virus Transfer Vector to Express Peste des Petits Ruminants H gene

[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.

人地交互视角下的阳产土楼聚落空间特征探析

以皖南古村中鲜为人知的另类山地聚落——阳产土楼为例,在环境行为研究领域多学科推动完善的人地交互理论导引下,结合具身研究与图像数据,以其发生发展的动力源——人与山地交互(transaction)为入手点,阐述阳产的聚落空间构成要素与山地聚落空间特征。从人工-自然2类空间构成要素的梳理开始,分析其动态交互式空间、静态交互式空间及动静组合的空间序列,揭示其空间特征对使用者具身行为的引导及情感关联的建立作用。从场所营建的视角挖掘阳产“因山制形”的营建智慧,提出“人地交互”作为一种理解、研究、感受与设计场地的具身式方法。由此,一方面完善此类徽州传统村落研究的不足,为其后续保护、更新与活化提供参考;另一方面也为数字时代的山地聚落空间设计给予启示。

Studies on the Extraction Methods of Metagenomic DNA from Mud Volcano in Xinjiang

[Objective]To seek one effective extraction method of metagenomic DNA from mud volcano.[Method]The metagenomic DNA from mud volcano was extracted by CTAB extraction method,SDS-enzyme method,improved method,reagent kit method.The extraction of four kinds of methods were compared.[Result]The extracted rate in reagent sets method was the highest,next was improved method,the extracted quantity in SDS-enzyme method was maximum.DNA extracted by the improved method was diluted ten times for PCR.[Conclusion]Considering economy and purity,the improved method can be used as one effective extraction method of metagenomic DNA from mud volcano.

Integrated interpretation of dual frequency induced polarization measurement based on wavelet analysis and metal factor methods

In mineral exploration, the apparent resistivity and apparent frequency （or apparent polarizability） parameters of induced polarization method are commonly utilized to describe the induced polarization anomaly. When the target geology structure is significantly complicated, these parameters would fail to reflect the nature of the anomaly source, and wrong conclusions may be obtained. A wavelet approach and a metal factor method were used to comprehensively interpret the induced polarization anomaly of complex geologic bodies in the Adi Bladia mine. Db5 wavelet basis was used to conduct two-scale decomposition and reconstruction, which effectively suppress the noise interference of greenschist facies regional metamorphism and magma intrusion, making energy concentrated and boundary problem unobservable. On the basis of that, the ore-induced anomaly was effectively extracted by the metal factor method.

Diversity of Bmp15 and Gdf9 Genes in White Goat of Guizhou Province and Evolution of the Encoded Proteins

Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation confirmed that the fecundity mutations of sheep presented in highly prolific White goat individuals of Guizhou province. To illuminate other polymorphisms in Bmpl5 and Gdfl） genes and the relationship of these mutations with function, we cloned and characterized the coding region of Bmp15 and Gdfl）. Molecular models of BMP15 and GDF9 mature peptide of White goat were constructed based on the homology of human BMP7 experimental tertiary structure. Two exons encoded prepropeptide of 394 amino acids in BMPI5 and 453 residues in GDF9, respectively. Apart from the FecXs mutation （S99I） in BMP15 and V791 mutation in GDF9 confirmed in White goat previously, other seven and three polymorphism sites were detected from BMP15 and GDF9 mature peptides, respectively. S32G, N66H, S99I/P99I and G107R in BMP15 could be important for the binding of dimer to receptors. Changes of P78Q and V79I in GDF9 might affect the binding of dimer to receptor type t. Comparing the length of BMP 15 and GDF9 prepropeptide in vertebrates, an increase in length of BMP 15 presented along with the protein evolution from fish to mammal and the divergence of the N-terminus residues in matured BMP15 peptide might contribute to the sensitive control on the fertility of animal species with low ovulation rate. These findings gave a valuable explanation for the correlation of mutations in Bmpl5 and Gdfl） genes with the control on fecundity of White goat and supported the notion that they were the pivotal factors in female fertility of White goat in Guizhou province.

Molecular Composition and Evolution of the Chalcone Synthase (CHS) Gene Family in Five Species of Camellia (Theaceae)

The molecular composition and evolution of the chalcone synthase (CHS) gene family from five species in Camellia (Theaceae) are explored in this study. Sixteen CHS exon 2 from four Camellia species were amplified from total DNA by PCR method. Three sequences of the fifth species in Camellia and two sequences of Glycine max as the designated outgroups were obtained from GenBank. Our results indicated that CHS gene family in Camellia was differentiated to three subfamilies (A, B, C) during the evolutionary history with six groups (A1, A2, A3, BI, B2, C). Among them, only group A2 was possessed by all five species in this study. However, the other five groups were detected only in some species of the plants studied. All members of CHS gene family in this study had high sequence similarity, more than 90% among the members in the same subfamily and more than 78% among different subfamilies at nucleotide level., According to the estimated components of amino acids, the function of CHS genes in Camellia had been diverged. The nucleotide substitutions of the different groups were not identical. Based on phylogenetic analyse inferred from sequences of CHS genes and their deduced amino acid sequences, we concluded that the CHS genes with new function in this genus were evolved either by mutations on several important sites or by accumulation of the mutations after the gene duplication. A further analysis showed that the diversification of CHS genes in Camellia still occurred recently, and the evolutionary models were different to some extant among different species. So we assumed that the different evolutionary models resulted from the impacts of variable environmental elements after the events of speciation.

Overexpression of IGF2R and IGF1R mRNA in SCNT-produced Goats Survived to Adulthood

The procedure of somatic cell nuclear transfer （SCNT） is likely to affect the expression level of growth-related genes especially imprinting genes. In this study, expressions of growth-related genes including three imprinting genes （H19, IGF2, and IGF2R） and four non-imprinting genes （IGF1, IGFIR, GHR, and GHSR） in adult nuclear transferred （NT） goats were investigated by real-time PCR. The expressions of these genes in adult clones were found largely normal, but IGF2R and IGFIR were more highly expressed in cloned goats than in non-NT goats （P 〈 0.01）. Analysis on mono-allelic expression pattern of imprinting genes indicated that mono-allelic expression patterns of H19 and IGF2 in cloned goats were similar to that in non-NT goats. In addition, the sequence of goat IGF2 gene and the putative amino acid sequence were obtained. The 986 nucleotide cDNA of goat IGF2 gene contained an open-reading frame of 540 nucleotides coding for 179 amino acids. Both cDNA sequence and amino acid sequence of IGF2 in goat showed their higher homology with that in sheep than in cattle; the partial cDNA fragments of H19, IGF2R, GHSR, IGFIR, and GHR in goat were also cloned and sequenced, which shared higher sequence identities with those in sheep than in cattle.

Habitat Utilization of the Sichuan Hill Partridge (Arborophila rufipectus) in the Non-breeding Period in Laojunshan Nature Reserve

The Sichuan Hill Partridge （Arborophila rufipectus ） requires successional broadleaf forest and their populations have declined as a result of fragmentation of endemic bird areas in subtropical forest in the mountains of southwestern China. In this paper, habitat utilization of the Sichuan Hill Partridge was studied in replanted broadleaf forests, in Laojunshan Nature Reserve of Sichuan, to determine the importance of habitat features, during the non-breeding period from November to December 2005. The Sichuan Hill Partridge utilized habitats within elevations of 1 000 to 1 600 m and with a south-facing slope of two to 15 degrees, close to road and forest edges. The birds preferred sites with smaller bamboo density, lower bamboo cover and snow cover and shrub cover was greater at used sites than at random sites. Principal components analysis indicated that food on the ground layer, topographic condition, concealment and temperature were the first four components of bird habitat selection, and the load of the first component was 29.407%. The findings indicated that the Sichuan Hill Partridge might face the well-documented trade-off between food resource and predation risk when utilizing habitat. We suggest that the conservation and restoration of successional broadleaf forest habitats will benefit the Sichuan Hill Partridge.

The Relationship between Genetic Polymorphisms of Three Gene Loci and Growth Characters of Haimen Goats

[Objective] The paper aims to study the relationship between genetic polymorphisms of three gene loci and growth character of Haimen Goats to provide reference to promote Haimen Goat growth character and cultivate Haimen Goat new strain of fast-growth.[Method] We processed analysis and related statistical analysis on three gene loci of Haimen Goats with PCR-RFLP and PCR-SSCP.[Result] The results suggested that there were two genotypes in MSTN gene IntronⅡ,GH gene exonⅠ and GH gene exonⅡ in Haimen Goats.Different genotypes of these gene loci had different effects on relative growth character of Haimen Goats.[Conclusion] This study had a significant meaning about promoting Haimen Goat avoirdupois by marker assistant option.

Cloning and Sequence Analysis of 5′ Flanking Region and Exon of Inhibin α Precursor Gene in Goats

[Objective] The aim of this study was to reveal the relationship between inihibin （INH） α precursor gene and seasonal reproduction of goats, and investigate the evolutionary conservation of INHα precursor gene. [ Method] Cloning and sequence analysis of 5＇ flanking region and exon of inihibinα （INHE） precursor gene in twenty ewes between non-seasonal estrous breed （Haimen goats） and seasonal estrous breed （Anhui white goats） was analyzed in this study. [ Result] Compared with Anhui white goats, INHα precursor gene in Haimen goats had three SNP but no amino acid change, while its nucleotide homology was 99.7% and amino acid homology was 100%. The nucleotide homology of INHα precursor gene in goat, cattle, pig, person, chicken, horse, rat and dog ranged from 12.7% to 96.5%. [ Conclusion] INHα precursor gene tends to be highly conserved in species, and any change of nucleotide and amino acid maybe directly influence the function of the whole gene coding and regulation.

春游九里沟

春游九里沟李林洲，刘静象一位久居深闺的少女，千娇百媚，却鲜为人知；似一处烟笼雾锁的仙境．出神入化．却闻者寥寥。这就是正在开发建设中的济源市九里沟风景区。一个春光明媚的日子，我们驱车来到济源市西北１５公里的九里沟。至郑坪下车，沿一条渡淮溪水运通而行三里...

Cloning,Sequence Analysis and Amino Acid Structure Prediction of TLR9 Gene from Wild Ovis ammon in Xinjiang

[Objective] The study aimed at cloning and identifying the toll receptor gene 9（TLR9） of wild Ovis ammon in Xinjiang,and predicting its structure and function.[Method] The TLR9 complete sequence of wild Ovis ammon was cloned from its peripheral blood by PCR technology.Then the PCR products were purified by agarose gel electrophoresis and then sequenced.Finally,the structure and function of TLR9 sequence were predicted by molecular biological software.[Result] The complete sequence of TLR9 gene was 3 192 bp in length,encoding 1 064 amino acids with a signal peptide composed of 30 amino acids;the leucine percentage reached as high as 18.5%.The TLR9 amino acid possibly contained three hydrophobic regions,at amino acids 455-475,740-760 and 780-800.The 3-D structure of TLR9 was constructed by the extracellular LRR domain and intracellular TIL domain（Toll/IL IR）.[Conclusion] The characteristics of TLR9 provided theoretical basis for further study on the TLR9 gene of wild Ovis ammon in Xinjiang.

Nutritional factors and gastric cancer in Zhoushan Islands, China

AIM: To investigate the association between nutrient intakes and high incidence rate of gastric cancer among residents in Zhoushan Islands.METHODS: A frequency-matched design of case-control study was used during the survey on dietary factors and gastric cancer in Zhoushan Islands, China. A total of 103 cases of gastric cancer diagnosed in 2001 were included in the study and 133 controls were randomly selected from the residents in Zhoushan Islands. A food frequency questionnaire was specifically designed for the Chinese dietary pattern to collect information on dietary intake. A computerized database of the dietary and other relative information of each participant was completed. Total calories and 15 nutrients were calculated according to the food composition table and their adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by gender using unconditional logistic regression models.RESULTS: High intakes of protein, saturated fat, and cholesterol were observed with the increased risk of gastric cancer particularly among males (ORQ4 vsQ1 were 10.3, 3.24, 2.76 respectively). While carbohydrate was a significant high-risk nutrient (ORQ4 vsQ1 = 14.8; Pfor linear trend = 0.024) among females. Regardless of their gender, the cases reported significantly higher daily intake of sodium mainly from salts. As to the nutrients of vitamins A and C, an inversed association with the risk of GC was found. Baseline characteristics of participants were briefly described.CONCLUSION: The findings from this study confirm the role of diet-related exposure in the etiology of gastric cancer from the point of view of epidemiology. An increased risk of gastric cancer is associated with high intakes of protein, saturated fat, cholesterol and sodium,while consumption of polyunsaturated fat, vitamin A and ascorbic acid may have a protective effect against gastric cancer.

Failure Mechanisms of Post-Earthquake Bedrock Landslides in Response to Rainfall Infiltration

Among the triggering factors of postearthquake bedrock landslides, rainfall plays an important role. However, with slope variation, the mechanism of its effects on the failure of rock landslides is not dear. Here, from the viewpoint of fracture mechanics, and based on post-earthquake conditions, the mechanisms of crack propagation, water infiltration and development of the sliding surface were investigated. Then, according to the upper boundary theorem, the effects of water infiltrated into fractures on the stability of rock slopes were analyzed quantitatively. Finally, an example is presented to verify the theory. The results show that the propagation and coalescence of cracks and the lubrication of incipient sliding surfaces are the main causes of the failure of post-earthquake rock landslides in response to rainfall.

Shallow groundwater dynamics in North China Plain

The groundwater level of 39 observation wells including 35 unconfined wells and 4 confined wells from 2004 to 2006 in North China Plain(NCP) was monitored using automatic groundwater monitoring data loggers KADEC-MIZU II of Japan.The automatic groundwater sensors were installed for the corporation project between China and Japan.Combined with the monitoring results from 2004 to 2006 with the major factors affecting the dynamic patterns of groundwater, such as topography and landform, depth of groundwater level, exploitation or discharge extent, rivers and lakes, the dynamic regions of NCP groundwater were gotten.According to the dynamic features of groundwater in NCP, six dynamic patterns of groundwater level were identified, including discharge pattern in the piedmont plain, lateral recharge-runoff-discharge pattern in the piedmont plain, recharge-discharge pattern in the central channel zone, precipitation infiltration-evaporation pattern in the shallow groundwater region of the central plain, lateral recharge-evaporation pattern in the recharge-affected area along the Yellow River and infiltration-discharge-evaporation pattern in the littoral plain.Based on this, the groundwater fluctuation features of various dynamic patterns were interpreted and the influencing factors of different dynamic patterns were compared.

Genesis of Qujiashan manganese deposit, Shaanxi Province: constraints from geological, geochemical, and carbon and oxygen isotopic evidences

The Qujiashan manganese deposit is located in the Longmen-Daba fold belt along the northern margin of the Yangtze Block. The layered ore bodies are distributed within the purple-red calcareous shale. Qujiashan is a high-grade w(MnO)=8.92% to 18.76%) manganese deposit with low-phosphorus w(P2O5)=0.08% to 0.16%) content. It also has a low total REEs contents(with an average of 101.3×10-6), and has inconspicuous Ce(0.81 to 1.29) and Eu(1.00 to 1.25) anomalies. lg(Ce/Ce*) values are from-0.02 to 0.11. The ores have high SiO2/Al2O3 and Al/(Al + Fe + Mn) ratios. In figures of Fe–Mn–[(Ni+Cu+Co)×10] and lgU–lgTh, all samples show that hydrothermal exhalative fluids played an important role during mineralisation. The δ13CPDB and δ18OSMOW values of eight ore samples are from-20.7‰ to-8.2‰(with an average of-12.4‰) and from 14.3‰ to 18.7‰(with an average of 17.0‰), respectively. These carbon and oxygen isotopic features indicate that hydrothermal fluids derived from deep earth are participation in the metallogenic process, which is also supported by high paleo-seawater temperatures varying from 47.08 to 73.98 °C. Therefore, the geological and geochemical evidences show that the Qujiashan deposit formed from submarine exhalative hydrothermal sedimentation.

PUSH-PULL FACTORS IN MOUNTAIN RESORTS——A Case Study of Huangshan Mountain as World Heritage

The push-pull framework provides a useful approach for examining the tourist motivation. This paper takes the world heritage-Huangshan Mountain as a sample. From the two different aspects of pull and push factors, the underlying features of visitors′ motives to Huangshan Mountain are analyzed with the help of factor analysis. As a result, five push factors and four pull factors are identified. Further analyses investigate differences in the push and pull factors among different socio-demographic subgroups with one-way ANOVA analysis. The result of the study affords us useful references for development, protection and marketing expansion of mountain resorts.