目的探讨人脐带间充质干细胞移植对围绝经期大鼠性激素水平的影响。方法阴道脱落细胞涂片法筛选出30只月龄13-15个月的SPF级SD围绝经期大鼠模型,随机分为A、B、C 3组（每组10只）,喂养方式相同。A组不予处理,B组经尾静脉移植浓度为1...目的探讨人脐带间充质干细胞移植对围绝经期大鼠性激素水平的影响。方法阴道脱落细胞涂片法筛选出30只月龄13-15个月的SPF级SD围绝经期大鼠模型,随机分为A、B、C 3组（每组10只）,喂养方式相同。A组不予处理,B组经尾静脉移植浓度为1×10^6cell/m L的人脐带间充质干细胞1 m L,隔日1次,共2次;C组与B组同一方法和时间移植PBS液1 m L。于成模时和末次移植后7和14 d检测各组大鼠性激素：雌二醇（E2）、孕酮（P）、黄体生成素（LH）、卵泡刺激素（FSH）的水平。结果 3组大鼠成模时及末次移植后7 d E2、P、FSH、LH水平比较差异无统计学意义（P〉0.05）;B组大鼠E2、P水平随移植时间延长呈上升趋势,FSH水平随移植时间延长呈下降趋势;末次移植后14 d E2、FSH、P水平与末次移植后7 d及移植前比较差异有统计学意义（P〈0.05）。B组末次移植后14 d与A组和C组相同实验时间段E2、P、FSH、LH水平比较差异有统计学意义（P〈0.05）;A组和C组不同实验时间段E2、P水平呈下降趋势,FSH、LH水平呈上升趋势,移植前与移植14 d后比较差异有统计学意义（P〈0.05）。结论人脐带间充质干细胞移植能改善围绝经期大鼠性激素水平,提高卵巢储备能力。展开更多
Objective： To investigate the effect of acupuncture on serum superoxide dismutase（SOD） and malondialdehyde（MDA） of rats in menopause. Methods： Female rats in menopause were randomly allocated into acupuncture, d...Objective： To investigate the effect of acupuncture on serum superoxide dismutase（SOD） and malondialdehyde（MDA） of rats in menopause. Methods： Female rats in menopause were randomly allocated into acupuncture, drugs and control groups, which were treated with acupuncture, Gengnian＇an Tablet, and nothing, respectively. In addition, some young female rats were used as control. After 17-day treatments, serum SOD and MDA were measured with biochemical methods. Results： Compared with the young rats, rats in menopause had lower contents of serum SOD（P〈0.01）, and higher contents of MDA（P〈0.01）. After acupuncture treatment, the contents of serum SOD increased（P〈0.05）, and MDA decreased（P〈0.05）. Conclusion： Acupuncture could increase the serum SOD contents and decrease the serum MDA contents of rats in menopause.展开更多
Objective： To explore the best opportunity for electroacupuncture therapy to treat perimenopause syndrome. Methods： The rats were duplicated as ovariectomized model and took the electroacupuncture therapy at 5 diffe...Objective： To explore the best opportunity for electroacupuncture therapy to treat perimenopause syndrome. Methods： The rats were duplicated as ovariectomized model and took the electroacupuncture therapy at 5 different periods. The heat-resistance ability, physical strength and sleeping ability of the rats at different intervention time were recorded. Results： For the rats with perimenopause syndrome at all periods, the electroacupuncture could promote the beat-resistance and the physical strength, shorten the sleep latency and prolong the sleeping time. The results of the rats in the 7 d pre-modeling group, 1 d post-modeling group and 8 d post-modeling group were the best, followed by the results of 15 d post-modeling group and 22 d post-modeling group. Conclusion： The prevention and early intervention of electroacupuncture on the rats with perimenopanse syndrome were satisfied and the effect declined with a later intervention time, indicating the importance of prevention and early intervention.展开更多
文摘目的探讨人脐带间充质干细胞移植对围绝经期大鼠性激素水平的影响。方法阴道脱落细胞涂片法筛选出30只月龄13-15个月的SPF级SD围绝经期大鼠模型,随机分为A、B、C 3组（每组10只）,喂养方式相同。A组不予处理,B组经尾静脉移植浓度为1×10^6cell/m L的人脐带间充质干细胞1 m L,隔日1次,共2次;C组与B组同一方法和时间移植PBS液1 m L。于成模时和末次移植后7和14 d检测各组大鼠性激素：雌二醇（E2）、孕酮（P）、黄体生成素（LH）、卵泡刺激素（FSH）的水平。结果 3组大鼠成模时及末次移植后7 d E2、P、FSH、LH水平比较差异无统计学意义（P〉0.05）;B组大鼠E2、P水平随移植时间延长呈上升趋势,FSH水平随移植时间延长呈下降趋势;末次移植后14 d E2、FSH、P水平与末次移植后7 d及移植前比较差异有统计学意义（P〈0.05）。B组末次移植后14 d与A组和C组相同实验时间段E2、P、FSH、LH水平比较差异有统计学意义（P〈0.05）;A组和C组不同实验时间段E2、P水平呈下降趋势,FSH、LH水平呈上升趋势,移植前与移植14 d后比较差异有统计学意义（P〈0.05）。结论人脐带间充质干细胞移植能改善围绝经期大鼠性激素水平,提高卵巢储备能力。
基金Education committee of Shanghai(03CK21)Shanghai Leading Academic Discipline Project(T0302)
文摘Objective： To investigate the effect of acupuncture on serum superoxide dismutase（SOD） and malondialdehyde（MDA） of rats in menopause. Methods： Female rats in menopause were randomly allocated into acupuncture, drugs and control groups, which were treated with acupuncture, Gengnian＇an Tablet, and nothing, respectively. In addition, some young female rats were used as control. After 17-day treatments, serum SOD and MDA were measured with biochemical methods. Results： Compared with the young rats, rats in menopause had lower contents of serum SOD（P〈0.01）, and higher contents of MDA（P〈0.01）. After acupuncture treatment, the contents of serum SOD increased（P〈0.05）, and MDA decreased（P〈0.05）. Conclusion： Acupuncture could increase the serum SOD contents and decrease the serum MDA contents of rats in menopause.
基金Key Project of Hangzhou Municipal Health Bureau(20072004)
文摘Objective： To explore the best opportunity for electroacupuncture therapy to treat perimenopause syndrome. Methods： The rats were duplicated as ovariectomized model and took the electroacupuncture therapy at 5 different periods. The heat-resistance ability, physical strength and sleeping ability of the rats at different intervention time were recorded. Results： For the rats with perimenopause syndrome at all periods, the electroacupuncture could promote the beat-resistance and the physical strength, shorten the sleep latency and prolong the sleeping time. The results of the rats in the 7 d pre-modeling group, 1 d post-modeling group and 8 d post-modeling group were the best, followed by the results of 15 d post-modeling group and 22 d post-modeling group. Conclusion： The prevention and early intervention of electroacupuncture on the rats with perimenopanse syndrome were satisfied and the effect declined with a later intervention time, indicating the importance of prevention and early intervention.
