Box-Behnken响应面法优化盆炎灵颗粒的醇沉工艺研究

目的优化盆炎灵颗粒的醇沉工艺。方法在单因素试验的基础上,以醇沉前液相对密度、乙醇终浓度与静置时间为考察因素,以绿原酸、芍药苷、阿魏酸、丹酚酸B、去甲异波尔定、甘草苷、延胡索乙素、落新妇苷的转移率与固体去除率的综合评分值为考察指标,采用基于Box-Behnken设计的响应面法优化盆炎灵颗粒的醇沉工艺。色谱条件:色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm),流速1.0 mL/min,流动相A为乙腈,流动相B为0.2%磷酸水溶液,梯度洗脱(0~10 min,2%A;10~40 min,2%~12%A;40~80 min,12%~18%A;80~110 min,18%~25%A;110~120 min,25%~50%A),柱温30℃,检测波长200~400 nm。结果最佳醇沉工艺条件为醇沉前液相对密度1.13,乙醇终浓度60%,静置时间21 h。验证试验结果显示,综合评分的预测值与实测值相对偏差为0.7%。结论优化的醇沉工艺方法简单、操作简便且结果准确稳定,可为盆炎灵颗粒的制剂生产提供可靠的理论依据。

微生物絮凝剂对油田采出液污水絮凝效果的研究

从油田采出液污水中筛选得到一株对三元复合驱采出液污水絮凝效果较好的微生物絮凝剂产生菌WZ,对WZ菌的16SrDNA进行了测序分析,考察了WZ菌代谢产物对三元复合驱采出液污水的絮凝效果,分析了WZ微生物絮凝剂的主要活性成分。结果表明,WZ菌株为成团泛菌(Pantoea agglomerans),其产生的絮凝剂主要分布在发酵液离心后的上清液中。35℃、170r/min摇床振荡培养29h,WZ微生物絮凝剂对采出液污水的絮凝效果最好,最适投加量为25mL/L,悬浮物去除率达到47.76%。经蒽酮比色法、考马斯亮蓝法和红外光谱法分析得知:WZ微生物絮凝剂主要成分为糖蛋白类物质。

Effect of Solid Retention and Hydraulic Retention Times on Membrane Fouling in Membrane Bioreactor

In this bench scale submerged membrane bioreactor, effect of solid retention time and hydraulic retention time on membrane fouling propensity has been studied. This experiment is carried out at different solid retention time of 5, 10, 30, 70 and 98 days; and fouling behavior of membrane bioreactor is investigated. Average effluent quality is found to be 88.14%-94.38%. The experiment with different hydraulic retention is carried for 3 to 4 days and fouling behavior has been investigated. The effluent quality at different hydraulic retention time of 1, 2, 3, 5, 10 and 24 hours has been investigated. This paper aims to search optimal values of solid and hydraulic retention times at which lower fouling and higher organic removal efficiency can be obtained. This study has been mainly focused on operating parameters rather than microbial structure, effect and analysis of activated sludge to membrane fouling.

一种新型低浊度含油污水反相破乳剂的应用性能评价

测试了一种新型的丙烯酸类反相破乳剂BH-10的应用效果。实验室含油污水破乳试验表明,该破乳剂除油率达到80%以上,固体悬浮颗粒含量去除率达到50%以上,具有较好的破乳效果。