在外重原子微扰剂Pb(Ac)2存在下,基于罗丹明6 G(Rhodamine 6G,简写为R)的SiO2纳米微球(简写为R-SiO2)在醋酸纤维素膜(ACM)上于λex/λem=482.38/648.59 nm处能发射强而稳定的室温燐光信号.由该发光微球标记的麦胚凝集素(Triticum vulgar...在外重原子微扰剂Pb(Ac)2存在下,基于罗丹明6 G(Rhodamine 6G,简写为R)的SiO2纳米微球(简写为R-SiO2)在醋酸纤维素膜(ACM)上于λex/λem=482.38/648.59 nm处能发射强而稳定的室温燐光信号.由该发光微球标记的麦胚凝集素(Triticum vulgare lectin简称WGA),不仅仍能在ACM上与碱性磷酸酶(AP)发生定量的特异性亲和吸附反应,且亲和吸附反应产物能发射更强的室温燐光(RTP)信号,据此建立了R-SiO2纳米微球标记凝集素(WGA)亲和吸附(AA)固体基质室温燐光法(AA-SS-RTP)测定AP的一种新方法.本法的线性范围为1.00～360.00 ag AP spot-1(样品体积0.4μL/斑,对应浓度2.50～900.00fg/ml)),工作曲线的回归方程为△Ip=16.24+0.8856 m AP/ag spot-1,r=0.9993.检出限为0.14ag spot-1.分别对1.00和360.00 ag spot-1 AP重复测定11次,RSD为3.9%和3.1.%.本方法灵敏、准确、精密度好,已成功用于人血清中AP的测定.展开更多
Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=...Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.展开更多
以NaCMC(羧甲基纤维素钠)为前驱体,掺杂水杨基荧光酮(THBF),Pb2+为沉淀剂,用Sol-gel法合成发光分子水杨基荧光酮羧甲基纤维素铅微球(简记为Pb(CMC)2–THBF).Pb(CMC)2–THBF在滤纸基质上可发射强而稳定的室温燐光(RTP).基于EDTA与Pb2+的...以NaCMC(羧甲基纤维素钠)为前驱体,掺杂水杨基荧光酮(THBF),Pb2+为沉淀剂,用Sol-gel法合成发光分子水杨基荧光酮羧甲基纤维素铅微球(简记为Pb(CMC)2–THBF).Pb(CMC)2–THBF在滤纸基质上可发射强而稳定的室温燐光(RTP).基于EDTA与Pb2+的络合反应,使Pb(CMC)2–THBF微球转化为水溶性组分(PbY2-、2CMC-、THBF),该水溶性组分与痕量上Pd2+作用生成(CMC)2 Pd–THBF,导致Pb(CMC)2–THBF上RTP猝灭,Pd2+上含量与△Ip值成线性关系,据此建立了Pb(CMC)2–THBF固体基质室温燐光猝灭燐上痕量钯上新方法.该方法上检出限为0.38fg/斑(对应浓度为9.5×10-13g/mL),工作曲线回归方程为△Ip=17.95+0.7847 C Pd2+(fg/斑),r=0.9989.本方法用于分子筛催化剂和黄矿石中痕量钯上燐上,结果满意.同时时时了固体基质室温燐光测定钯的反应机理.展开更多
文摘在外重原子微扰剂Pb(Ac)2存在下,基于罗丹明6 G(Rhodamine 6G,简写为R)的SiO2纳米微球(简写为R-SiO2)在醋酸纤维素膜(ACM)上于λex/λem=482.38/648.59 nm处能发射强而稳定的室温燐光信号.由该发光微球标记的麦胚凝集素(Triticum vulgare lectin简称WGA),不仅仍能在ACM上与碱性磷酸酶(AP)发生定量的特异性亲和吸附反应,且亲和吸附反应产物能发射更强的室温燐光(RTP)信号,据此建立了R-SiO2纳米微球标记凝集素(WGA)亲和吸附(AA)固体基质室温燐光法(AA-SS-RTP)测定AP的一种新方法.本法的线性范围为1.00～360.00 ag AP spot-1(样品体积0.4μL/斑,对应浓度2.50～900.00fg/ml)),工作曲线的回归方程为△Ip=16.24+0.8856 m AP/ag spot-1,r=0.9993.检出限为0.14ag spot-1.分别对1.00和360.00 ag spot-1 AP重复测定11次,RSD为3.9%和3.1.%.本方法灵敏、准确、精密度好,已成功用于人血清中AP的测定.
文摘Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.
文摘以NaCMC(羧甲基纤维素钠)为前驱体,掺杂水杨基荧光酮(THBF),Pb2+为沉淀剂,用Sol-gel法合成发光分子水杨基荧光酮羧甲基纤维素铅微球(简记为Pb(CMC)2–THBF).Pb(CMC)2–THBF在滤纸基质上可发射强而稳定的室温燐光(RTP).基于EDTA与Pb2+的络合反应,使Pb(CMC)2–THBF微球转化为水溶性组分(PbY2-、2CMC-、THBF),该水溶性组分与痕量上Pd2+作用生成(CMC)2 Pd–THBF,导致Pb(CMC)2–THBF上RTP猝灭,Pd2+上含量与△Ip值成线性关系,据此建立了Pb(CMC)2–THBF固体基质室温燐光猝灭燐上痕量钯上新方法.该方法上检出限为0.38fg/斑(对应浓度为9.5×10-13g/mL),工作曲线回归方程为△Ip=17.95+0.7847 C Pd2+(fg/斑),r=0.9989.本方法用于分子筛催化剂和黄矿石中痕量钯上燐上,结果满意.同时时时了固体基质室温燐光测定钯的反应机理.
