To study the mechanism of SO2 and Hg removal from flue gas, an experimental packed bed reactor was designed to simulate the dry FGD, where a mixture of lime and fly ash in ratio 1：3 w/w was used as the S02 and Hg sor...To study the mechanism of SO2 and Hg removal from flue gas, an experimental packed bed reactor was designed to simulate the dry FGD, where a mixture of lime and fly ash in ratio 1：3 w/w was used as the S02 and Hg sorbent, and steam at temperature of 100 ℃ was applied for activation of the sorbent, while the activation time set to 20 rain. The experimental factors including the SO2/Hg sorbent characteristics, 50% breakthrough time for SO2/Hg removal, sorbent packed bed depth and reaction temperature were investigated. The experimental results show that after steam activation, the BET specific surface area and specific pore volume increased from 37.8 to 45.5 m^2/g and from 0.42 to 0.51 cm^3/g, respectively. With activation of the sorbent by steam, the 50% breakthrough times of SO2 and Hg removal increased from 34 to 42 rain and from 23 to 45 rain, respectively. When the packed bed depth was increased from 5 to 25 ram. the 50% breakthrough times for Hg and S02 removal increased from 12 to 52 rain and from 6 to 47 rain, respectively. With the increase of the reaction temperature, the 50% breakthrough of SO2/Hg removal decreased accordingly. Steam activation can efficiently improve SO2/Hg removal simultaneously.展开更多
The collapsibility of loess ground can directly affect stability of subgrade. Therefore, how to adopt practical technical measures to reduce or eliminate its collapse deformation is an important content in foundation ...The collapsibility of loess ground can directly affect stability of subgrade. Therefore, how to adopt practical technical measures to reduce or eliminate its collapse deformation is an important content in foundation design in collapsible loess zone. Selecting collapsible loess from Fuxin-Chaoyang highway in Liaoning, the authors conducted a series of tests for improving loess with cement. The loess in different water content was mixed with the cement in varying proportions, unconfined compression strength for the samples at four different curing periods were tested, and the relationships of improved soil strength among cement mixture ratio and curing periods were analyzed. When the curing periods are certain, the strength of loess increases along with the mixture ratio increases; when the cement mixture ratio is 5%-15%, the scope of increases is quite obvious; when the mixture ratio is greater than 15%, the tendency of intensity increases turns slow. When the mixture ratio for the specimen is certain, the intensity of the test specimen increases along with the curing period increases, the intensity grows obviously in 28 days, and the growth rate is small in 28-90 days, the intensity tends to be steady in the curing period of 90 days.展开更多
Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for inc...Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for increasing digestion efficiency and de- creasing missed cleavages, which results in more peptides that are compatible with mass-spectrometry analysis. Compared to conventional solution digestion, immobilized protease digestion has the obvious advantages of short digestion time, no self-proteolysis, and reusability. We proposed a multiple-immobilized proteases-digestion strategy that combines the ad- vantages of the two digestion strategies mentioned above. Graphene-oxide （GO）-based immobilized trypsin and endoprotein- ase Glu-C were prepared by covalently attaching them onto the GO surface. The prepared GO-trypsin and GO-Glu-C were successfully applied in standard protein digestion and multiple immobilized proteases digestion of total proteins of Thermoan- aerobacter tengcongensis. Compared to 12-hour solution digestion using trypsin or Glu-C, 14% and 7% improvement were obtained, respectively, in the sequence coverage of BSA by one-minute digestion using GO-trypsin and GO-GIu-C. Multiple immobilized-proteases digestion of the total proteins of Thermoanaerobacter tengcongensis showed 24.3% and 48.7% en- hancement in the numbers of identified proteins than was obtained using GO-trypsin or GO-Glu-C alone. The ultra-fast and highly efficient digestion can be contributed to the high loading capacity of protease on GO, which leads to fewer missed cleavages and more complete digestion. As a result, improved protein identification and sequence coverage can be expected.展开更多
基金supported by the National High-Tech R&D Program of China (No. 2008AA06Z318)the Ministry of Environmental Protection of China (Nos. 201009048 and 200909025)
文摘To study the mechanism of SO2 and Hg removal from flue gas, an experimental packed bed reactor was designed to simulate the dry FGD, where a mixture of lime and fly ash in ratio 1：3 w/w was used as the S02 and Hg sorbent, and steam at temperature of 100 ℃ was applied for activation of the sorbent, while the activation time set to 20 rain. The experimental factors including the SO2/Hg sorbent characteristics, 50% breakthrough time for SO2/Hg removal, sorbent packed bed depth and reaction temperature were investigated. The experimental results show that after steam activation, the BET specific surface area and specific pore volume increased from 37.8 to 45.5 m^2/g and from 0.42 to 0.51 cm^3/g, respectively. With activation of the sorbent by steam, the 50% breakthrough times of SO2 and Hg removal increased from 34 to 42 rain and from 23 to 45 rain, respectively. When the packed bed depth was increased from 5 to 25 ram. the 50% breakthrough times for Hg and S02 removal increased from 12 to 52 rain and from 6 to 47 rain, respectively. With the increase of the reaction temperature, the 50% breakthrough of SO2/Hg removal decreased accordingly. Steam activation can efficiently improve SO2/Hg removal simultaneously.
基金Project supported by Natural Science Foundation of China (No. 40972171)
文摘The collapsibility of loess ground can directly affect stability of subgrade. Therefore, how to adopt practical technical measures to reduce or eliminate its collapse deformation is an important content in foundation design in collapsible loess zone. Selecting collapsible loess from Fuxin-Chaoyang highway in Liaoning, the authors conducted a series of tests for improving loess with cement. The loess in different water content was mixed with the cement in varying proportions, unconfined compression strength for the samples at four different curing periods were tested, and the relationships of improved soil strength among cement mixture ratio and curing periods were analyzed. When the curing periods are certain, the strength of loess increases along with the mixture ratio increases; when the cement mixture ratio is 5%-15%, the scope of increases is quite obvious; when the mixture ratio is greater than 15%, the tendency of intensity increases turns slow. When the mixture ratio for the specimen is certain, the intensity of the test specimen increases along with the curing period increases, the intensity grows obviously in 28 days, and the growth rate is small in 28-90 days, the intensity tends to be steady in the curing period of 90 days.
基金supported by the National Basic Research Program of China(2013CB911204)the National Key Scientific Instrument Development Program of China(2011YQ09000504)the National Natural Science Foundation of China(21275005,21235001)
文摘Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for increasing digestion efficiency and de- creasing missed cleavages, which results in more peptides that are compatible with mass-spectrometry analysis. Compared to conventional solution digestion, immobilized protease digestion has the obvious advantages of short digestion time, no self-proteolysis, and reusability. We proposed a multiple-immobilized proteases-digestion strategy that combines the ad- vantages of the two digestion strategies mentioned above. Graphene-oxide （GO）-based immobilized trypsin and endoprotein- ase Glu-C were prepared by covalently attaching them onto the GO surface. The prepared GO-trypsin and GO-Glu-C were successfully applied in standard protein digestion and multiple immobilized proteases digestion of total proteins of Thermoan- aerobacter tengcongensis. Compared to 12-hour solution digestion using trypsin or Glu-C, 14% and 7% improvement were obtained, respectively, in the sequence coverage of BSA by one-minute digestion using GO-trypsin and GO-GIu-C. Multiple immobilized-proteases digestion of the total proteins of Thermoanaerobacter tengcongensis showed 24.3% and 48.7% en- hancement in the numbers of identified proteins than was obtained using GO-trypsin or GO-Glu-C alone. The ultra-fast and highly efficient digestion can be contributed to the high loading capacity of protease on GO, which leads to fewer missed cleavages and more complete digestion. As a result, improved protein identification and sequence coverage can be expected.
