Aim To develop and validate a RP-HPLC method for the analysis of paclitaxel in a solid dispersion. Methods Paclitaxel and the internal standard norethisterone were separated using a Phenomenex ODS 3 column and monitor...Aim To develop and validate a RP-HPLC method for the analysis of paclitaxel in a solid dispersion. Methods Paclitaxel and the internal standard norethisterone were separated using a Phenomenex ODS 3 column and monitored at a wavelength at 227 nm. The isocratic mobile phase consisting of methanol-acetonitrile-water （40：30：30, V/V） was pumped at a flow-rate of 1.0 mL·min^-1. The dissolution studies were performed according to published studies. Results Under these chromatographic conditions, the calibration curve was linear in the range of 4-40 μg·mL ^-1 with the correlation coefficient of 0.9999. The mean recovery was 98.42 % （RSD = 1.19 %）. At the 60 min time point, the dissolution of paclitaxel from the solid dispersion was nearly 100 %, however, the original form of paclitaxel was about 30 %. Conclusion The method was proven to be specific, accurate and precise for determining the dissolution of paclitaxel from solid dispersion.展开更多
Particles(including solid particles,liquid drops and gas bubbles)are ubiquitous in a large number of natural processes as well as in industrial productions.Their behaviors are of fundamental importance in multiphase s...Particles(including solid particles,liquid drops and gas bubbles)are ubiquitous in a large number of natural processes as well as in industrial productions.Their behaviors are of fundamental importance in multiphase systems since the existence of such dispersed particles influences the momentum,mass and heat transport behaviors in these systems.Up to now,a vast body of literature has been published in dealing with the transport phenomena related to a particle surrounded by a fluid under various physical circumstances.In this paper,principal research results for the transport process of a single spherical particle in pure extensional and simple shear flows presented in the literature,including our recent work,are generally reviewed in order to give a comprehensive knowledge in this area.展开更多
文摘Aim To develop and validate a RP-HPLC method for the analysis of paclitaxel in a solid dispersion. Methods Paclitaxel and the internal standard norethisterone were separated using a Phenomenex ODS 3 column and monitored at a wavelength at 227 nm. The isocratic mobile phase consisting of methanol-acetonitrile-water （40：30：30, V/V） was pumped at a flow-rate of 1.0 mL·min^-1. The dissolution studies were performed according to published studies. Results Under these chromatographic conditions, the calibration curve was linear in the range of 4-40 μg·mL ^-1 with the correlation coefficient of 0.9999. The mean recovery was 98.42 % （RSD = 1.19 %）. At the 60 min time point, the dissolution of paclitaxel from the solid dispersion was nearly 100 %, however, the original form of paclitaxel was about 30 %. Conclusion The method was proven to be specific, accurate and precise for determining the dissolution of paclitaxel from solid dispersion.
基金supported by the National Science Fund for Distinguished Young Scholars(21025627)the National Natural Science Foundation of China(20990224,21106150)+1 种基金the National Basic Research Program of China(2010CB630904)863 project(2012AA03A606)
文摘Particles(including solid particles,liquid drops and gas bubbles)are ubiquitous in a large number of natural processes as well as in industrial productions.Their behaviors are of fundamental importance in multiphase systems since the existence of such dispersed particles influences the momentum,mass and heat transport behaviors in these systems.Up to now,a vast body of literature has been published in dealing with the transport phenomena related to a particle surrounded by a fluid under various physical circumstances.In this paper,principal research results for the transport process of a single spherical particle in pure extensional and simple shear flows presented in the literature,including our recent work,are generally reviewed in order to give a comprehensive knowledge in this area.
