通过固相萃取富集伏马菌素,采用液相色谱-电喷雾串联质谱法测定玉米和芦笋中的伏马菌素B1(FB1)、伏马菌素B2(FB2).在最佳色谱条件下,伏马菌素B1、伏马菌素B2的保留时间分别为5.2 min 和8.8 min,样品空白无干扰.离子化技术采用电喷雾正...通过固相萃取富集伏马菌素,采用液相色谱-电喷雾串联质谱法测定玉米和芦笋中的伏马菌素B1(FB1)、伏马菌素B2(FB2).在最佳色谱条件下,伏马菌素B1、伏马菌素B2的保留时间分别为5.2 min 和8.8 min,样品空白无干扰.离子化技术采用电喷雾正离子方式,以多反应监测(MRM)方式进行检测.该法检出限为80 pg;线性定量范围为0.01～10mg/L;回收率为78.3%～104.9%;相对标准偏差为2.2%～14.2%.利用ESI/MS/MS对伏马菌素B1、伏马菌素B2进行了质谱解析,分别选择特征离子峰m/z 352、528和m/z 336、512作为伏马菌素B1、伏马菌素B2准确定性的依据.方法灵敏度高,选择性好,准确度高,可为食品中伏马菌素的风险评估提供灵敏、准确的分析方法.展开更多
Objective: The aim of this study was to investigate the effect of polycyclic aromatic hydrocarbons (PAHs) in rectal carcinoma and hepatocarcinoma genesis. Methods: The PAHs in the human rectal cancer and liver cancer ...Objective: The aim of this study was to investigate the effect of polycyclic aromatic hydrocarbons (PAHs) in rectal carcinoma and hepatocarcinoma genesis. Methods: The PAHs in the human rectal cancer and liver cancer tissues, the adjacent tissues and homologous tissues without rectal cancer or liver cancer were extracted by ultrasonic wave. The extracts were then cleaned up and enriched by solid phase extraction, analyzed by high performance liquid chromatography (HPLC) with fluorescence spectroscopy. Results: Four kinds of PAHs were detected in human rectal and hepatic tissues. The contents of pyrene, 2-methylanthracene and benzo (a) pyrene in both rectal cancer tissues and adjacent homologous tissues were higher than rectal tissues without rectal cancer, the differences were statistically significant (P < 0.05). The contents of phenanthrene in the three kind of tissue were not significant (P > 0.05). The differences of the content of each PAHs between rectal cancer and adjacent tissue were not significant (P > 0.05). The contents of the four PAHs in the three kinds of liver tissues were not statistically significant (P > 0.05). Conclusion: PAHs are found in human rectal tissues or hepatic tissues. The contents of PAHs in human rectal tissue may have an effect on the occurrence of human rectal cancer while the contents of PAHs in human hepatic tissues may have not ones.展开更多
The estrogenic activity of influents and effluents of five municipal sewage treatment works （STWs） in Nanjing was investigated. The water samples were enriched by solid phase extraction, then eluted with different p...The estrogenic activity of influents and effluents of five municipal sewage treatment works （STWs） in Nanjing was investigated. The water samples were enriched by solid phase extraction, then eluted with different polar solvents, and f＇mally three fractionated extracts were obtained. Estrogen receptor recombinant yeast screen was used to evaluate the estrogenic activity of individual fractionated extracts and their mixture. The results show that the influents and effluents of the two STWs mainly receiving industrial wastewater have no obvious estrogenic activity, and the water samples from the other three STWs mainly receiving domestic wastewater show different degrees of estrogenic activity. The total estrogenic concentration ranged from 171. 52 ng/L EEQ （E2 - equivalent concentration） to 1 008.37 ng/L EEQ in the influents, and from ND （not detected） to 36.3 ng/L EEQ in the effluents. By comparing the results of different fractionated extracts, the methanol and dichloromethane extracts of the water samples show higher estrogenic activity, while the hexane extracts very low. The （xeno）estrogens from domestic wastewater could be removed obviously by activated sludge treatment units. However, the effluents of STWs in Nanjing still have chemical contaminants capable of binding to estrogen receptors.展开更多
基金Supported by a grant from Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology(No.200902)
文摘Objective: The aim of this study was to investigate the effect of polycyclic aromatic hydrocarbons (PAHs) in rectal carcinoma and hepatocarcinoma genesis. Methods: The PAHs in the human rectal cancer and liver cancer tissues, the adjacent tissues and homologous tissues without rectal cancer or liver cancer were extracted by ultrasonic wave. The extracts were then cleaned up and enriched by solid phase extraction, analyzed by high performance liquid chromatography (HPLC) with fluorescence spectroscopy. Results: Four kinds of PAHs were detected in human rectal and hepatic tissues. The contents of pyrene, 2-methylanthracene and benzo (a) pyrene in both rectal cancer tissues and adjacent homologous tissues were higher than rectal tissues without rectal cancer, the differences were statistically significant (P < 0.05). The contents of phenanthrene in the three kind of tissue were not significant (P > 0.05). The differences of the content of each PAHs between rectal cancer and adjacent tissue were not significant (P > 0.05). The contents of the four PAHs in the three kinds of liver tissues were not statistically significant (P > 0.05). Conclusion: PAHs are found in human rectal tissues or hepatic tissues. The contents of PAHs in human rectal tissue may have an effect on the occurrence of human rectal cancer while the contents of PAHs in human hepatic tissues may have not ones.
基金Program for New Century Excellent Talents in University of China(No.05-0481)
文摘The estrogenic activity of influents and effluents of five municipal sewage treatment works （STWs） in Nanjing was investigated. The water samples were enriched by solid phase extraction, then eluted with different polar solvents, and f＇mally three fractionated extracts were obtained. Estrogen receptor recombinant yeast screen was used to evaluate the estrogenic activity of individual fractionated extracts and their mixture. The results show that the influents and effluents of the two STWs mainly receiving industrial wastewater have no obvious estrogenic activity, and the water samples from the other three STWs mainly receiving domestic wastewater show different degrees of estrogenic activity. The total estrogenic concentration ranged from 171. 52 ng/L EEQ （E2 - equivalent concentration） to 1 008.37 ng/L EEQ in the influents, and from ND （not detected） to 36.3 ng/L EEQ in the effluents. By comparing the results of different fractionated extracts, the methanol and dichloromethane extracts of the water samples show higher estrogenic activity, while the hexane extracts very low. The （xeno）estrogens from domestic wastewater could be removed obviously by activated sludge treatment units. However, the effluents of STWs in Nanjing still have chemical contaminants capable of binding to estrogen receptors.
